01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Relatório de Estágio Supervisionado Obrigatório - Rancho Alta Tensão(2025-02-14) Freitas Filho, José Gildo Rufino de; Ferreira, Marcelo de Andrade; http://lattes.cnpq.br/4818123702136736; http://lattes.cnpq.br/5455362057251028Item Avaliação do fluxo sanguíneo do folículo ovulatório e corpo lúteo de fêmeas bubalinas por ultrassonografia Doppler(2024-09-27) Farias Júnior, Francisco Timbó; Batista, André Mariano; http://lattes.cnpq.br/5615914349535394; http://lattes.cnpq.br/7562129132084802The Mandatory Supervised Internship (ESO) is an essential part of the training of a Veterinary Doctor, being a mandatory requirement in the Veterinary Medicine course at UFRPE. With a workload of 420 hours, the internship was conducted at the Laboratory of Biotechniques Applied to Reproduction (LBR), between 04/01/2024 and 06/14/2024. The focus was on improving reproductive biotechnology, with emphasis on the in vitro maturation of oocytes and the development of an article based on research carried out with Murrah buffaloes. The study evaluated the blood flow of the ovulatory follicle and corpus luteum using Doppler ultrasound, correlating these data with reproductive efficiency. In the laboratory, activities such as in vitro maturation of bovine oocytes (IVM) and evaluation of meiotic progression using epifluorescence microscopy were carried out. Under the guidance of Professor Dr. André Mariano Batista and supervision of Professor Dr. Cláudio Coutinho Bartolomeu, the internship provided a practical insights into reproductive biotechnologies and their impact on reproductive efficiency in buffaloes.Item Trabalho de conclusão de residência: área de concentração – clínica médica, cirúrgica e da reprodução de grandes animais(2024-02-21) Silva, Edvaldo Sebastião da; Rizzo, Huber; http://lattes.cnpq.br/6985492817773613; http://lattes.cnpq.br/8172120694472135The Multi-professional Residency Programs and in the Health Professional Area are a specific teaching modality, aimed at health professions, which seek teaching-service-community integration, configuring an extremely important stage in the career of health professionals, allowing for better training through in-service training, thus delivering a more qualified professional to the job market. The activities aimed at the area of Medical, Surgical and Reproduction of Large Animals allowed the provision of services aimed at equine, swine, bovine, goat, sheep and buffalo species, contributing to the development and improvement of skills inherent to the profession of Veterinary Doctor. The present work aims to describe the activities performed at PRAPSMV, during the period from March 2022 to January 2022 and report a case of esophageal obstruction in a horse undergoing clinical-surgical treatment. The total number of services carried out in the period between March 2022 and January 2024 was 227 clinical, surgical and reproductive services, including all species treated in the area, of which 100 were ruminants, 127 horses and 4 pigs. With varying levels of complexity and affecting the different organic systems, broad and qualified training was possible, which is important for the professionalization of the Veterinary Doctor. In the context of the SUS, the work developed allowed for various learnings related to Public Health, making it possible to observe the main demands of the population, the activities carried out by Environmental, Epidemiological and Health Surveillance, as well as the importance of the Veterinary Doctor's role as a health professional. at NASF. It is concluded that the residency was extremely important for professional development, obtaining the necessary qualifications and confidence to offer specialized service to the job market, aimed at prevention, treatment and well-being of animals and the population in general.Item Relatório de Estágio Obrigatório Supervisionado: Fazenda Experimental Agroceres Multimix(2024-03-04) Ferreira, Danielly Alessandra Botelho de Almeida; Ferreira, Marcelo de Andrade; http://lattes.cnpq.br/4818123702136736; http://lattes.cnpq.br/9455481764669747Item Sucesso produtivo da indução de estro com prostaglandina em ovelhas da raça Santa Inês: relato de caso(2024-02-27) Aquino, Nelina Aurora Lourenço de; Souza, Andreia Fernandes de; Monnerat, João Paulo Ismério dos Santos; http://lattes.cnpq.br/3851426263880079; http://lattes.cnpq.br/6354486109796073The Santa Inês breed is a sheep breed widely used in Brazil, this factor is due to the adaptability and prolificacy of the breed, which on average is 1.4 lambs every two years. One of the techniques being studied are natural estrus inductions associated with the use of pharmacological protocols, including prostaglandin + male effect, which both stimulates follicular growth and also synchronizes estrus in this species. The objective of this course completion work was to report the experience of using prostaglandin in inducing estrus in Santa Inês sheep raised in the Department of Animal Husbandry at the Federal Rural University of Pernambuco - SEDE. We used 28 Santa Inês females raised in a confinement system, fed a diet based on (corn, wheat and soy) 200 g of concentrate/day, Tifton hay, water and mineral salt ad libitum. For estrus synchronization/induction, the animals were arranged in three groups (10, 10 and 8 animals), in order not to overload the breeders. To induce estrus, 0.3 ml of Cloprostenol Sodium (CIOSIN) was administered intramuscularly and repeated after seven days. After 48-72h the females were subjected to natural mating. Pregnancy diagnosis was performed using a trans-rectal ultrasound examination (ALOKA 100), starting on the 35th day after mating. 27 females were able to enter the estrus induction program. From the semiological evaluation, the females were classified according to their body condition score (BCS), where they presented scores between the ranges of 2.5 and 3.5. Regarding the observation of estrus, 100% of the females entered estrus after the second dose of PGF2a, on average 48h after the second application of the hormone. The conception rate showed a percentage of 88,88 % and the fertility rate was 81.48% (22/27). The birth rate was 181.81%, the total number of lambs born was 40 animals (22 males and 18 females), with an average weight of 3.365kg, with a lambing interval of 21 days between the first and last female. submitted to the protocol without transfer. The use of reproductive biotechnology is a viable alternative, as it allows births to be scheduled for a period that meets the needs of researchers from the Department of Animal Science at UFRPE-SEDE. The option to choose the protocol using prostaglandin F2a (PGF2a) was based on practicality and efficiency, as the calving period was reduced and the herd's birth rate increased, meeting our needs.Item Relatório de Estágio Supervisionado Obrigatório (ESO) realizado na Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Gado de Leite, Juiz de Fora e no Laboratório de Biotécnicas Aplicadas à Reprodução do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco: efeito da adição de nanoemulsão à base de óleo essencial rico em limoneno ao diluente de refrigeração de sêmen caprino(2024-02-15) Nottar, Luiz Henrique Todescatt; Batista, André Mariano; http://lattes.cnpq.br/5615914349535394; http://lattes.cnpq.br/3747311074315408The present study aims to report the activities carried out in compliance with the discipline 08525 - Mandatory Supervised Internship (MSI), part of the Bachelor's Degree in Veterinary Medicine, conducted between October 2, 2023, and November 28, 2023, at the Brazilian Agricultural Research Corporation (Embrapa), in the Dairy Cattle unit in the municipality of Juiz de Fora - MG, and between January 2 and January 19, 2024, at the Laboratory of Biotechniques Applied to Reproduction (LBR) of the Department of Veterinary Medicine (DMV) of the Federal Rural University of Pernambuco (UFRPE). The activities carried out provided in-depth training in in vitro embryo production (IVP) techniques for research purposes, including follicular aspiration of ovaries from abattoirs, in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro embryo culture (IVC). Activities in the field of molecular biology involving DNA extraction and amplification through the Polymerase Chain Reaction (PCR) technique were also performed. In the second chapter of this study, a research was conducted to evaluate the effect of adding nanoemulsion based on limonene-rich essential oil extracted from wild orange peel (NELS) to the semen extender of goat breeders. Seminal samples from five Saanen breed goats were used for the research. Each seminal pool was diluted in Tris-egg yolk supplemented with NELS at concentrations of 1.25, 2.5, and 5% at a concentration of 200 × 106 sperm/mL. The samples were placed in Eppendorf tubes (1.5 mL) and stored in refrigeration boxes (botuflex®) for cooling curve determination up to 5 ºC, and after 2 hours, they were transferred to the refrigerator, also at a controlled temperature of 5 ºC. For each analysis, part of each sample was aliquoted and transferred to other Eppendorf tubes (1.5 mL) in a dry bath at 37 ºC and subsequently evaluated in the Computer-Assisted Sperm Analysis System (CASA) for sperm kinetics, and in epifluorescence microscopy for Mitochondrial Membrane Potential (MMP), Plasma Membrane Integrity (PMI), and Acrosomal Membrane Integrity (AMI). The addition of wild orange nanoemulsion to the Tris-egg yolk extender was not able to improve sperm motility and kinetic parameters, besides significantly reducing MMP after 24 hours of refrigeration.Item Relatório de Estágio Supervisionado Obrigatório, realizado na Clínica Univet Saúde Animal em Santa Cruz do Capibaribe - PE. Relato de caso: hidropsia dos envoltórios fetal(2024-03-08) Marques, Mariana Keylla Gonçalves; Bartolomeu, Cláudio Coutinho; http://lattes.cnpq.br/7619056086707716; http://lattes.cnpq.br/6721768684359231Item Uso da gonadotrofina coriônica equina para melhora dos resultados da IATF em fêmeas Nelore(2024-03-04) Botelho, Caio Cézar Vergetti Albuquerque; Bartolomeu, Cláudio Coutinho; http://lattes.cnpq.br/7619056086707716This activity was carried out on several farms in the interior of Pernambuco from 10/02/2023 to 12/21/2023 with the aim of improving knowledge in the field area, such as a medical clinic and a laboratory analyzing brucellosis tests in cattle. In horses, it was only used in the medical-clinical area. The biggest focus was on bovine reproduction with fixed-time artificial insemination (IATF). During the internship period, farms in the forest and rural areas of Pernambuco were visited, mainly working with beef cattle. Gestational diagnoses were made with several different age ranges, protocol initiations, and artificial inseminations. In the surgical clinic area, surgeries such as castration in horses, vulvoplasty in heifers, and hematoma drainage in calves were experienced. Laboratory and field tests were carried out, with laboratory tests for brucellosis using the AAT (buffered acidified antigen) method and field tests focused on tuberculosis. At the animal exhibition, it was possible to receive documents (GTA, brucellosis test, equine infectious anemia test, and vaccination card) to receive the animals that arrived at the Cordeiro exhibition park. The birth of a Girolando female was observed. Chapter II included a literature review with the title “Use of equine chorionic gonadotropin to improve TAI results in Nelore females” in which the work reaffirms the effectiveness of using eCG in postpartum cows with low body scores, helping TAI protocols as a tool to enhance and improve follicular development and maturation.Item Comparação entre quatro protocolos de preparação do plasma rico em plaquetas na espécie equina(2024-02-02) Xavier, Gabriela Reis; Carneiro, Gustavo Ferrer; http://lattes.cnpq.br/0903050581306709; http://lattes.cnpq.br/3490771668260807The presente work aims to describe the activities carried out during the compulsory supervised internship, a compulsory subject for completion of the Veterinary Medicine course at the Federal Rural University of Pernambuco. The internship was initially carried out at the Fazenda Sucupira experimental field - Embrapa Recursos genéticos e Biotecnologias, Km 5 of DF 001, Riacho Fundo II and was then completed at the Animal Andrology Laboratory, in the Department of Veterinary Medicine at the Federal Rural University of Pernambuco (ANDROLAB/DMV/UFRPE), under the guidance of Prof. Dr. Gustavo Ferrer Carneiro during the period from 02/11/2023 to 16/01/2024. Throughout the internship, it was possible to participate in the routine of Fazenda Sucupira and the ANDROLAB routine, accompanying and assisting in carrying out the equine reproduction experiment, with an emphasis on follicular control, artificial insemination in horses, equine embryo lavage, semen collection and freezing. It was also possible to follow other research activities carried out at the internship site and with other species. The scientific article was written to compare four protocols for obtention platelet-rich plasma (PRP) in horses.Item Caracterização das propriedades químicas e biológicas do muco cloacal das tartarugas marinhas - Ipojuca/PE(2018-08-24) Souza, Daliana Thaisa Maria Teles de Oliveira; Santos, Ednilza Maranhão dos; Oliveira, Lourinalda Luiza Dantas da Silva Selva de; http://lattes.cnpq.br/5812920432455297; http://lattes.cnpq.br/4538607373477161Sea turtles are migratory animals that travel long distances and have a complex life cycle. They are bioindicators and biocontrol animals, contributing to the maintenance of the marine ecosystem. These animals exert great effort in choosing the spawning site to ensure reproductive success. Of the seven species that exist in the world, five occur in Brazil, and all are classified at some level of threat of extinction. There are many threats that turtles face in order to reach adulthood, one of which is related to marine pollution due to chemical compounds that have become an increasingly common reality for these animals. At the time of spawning the turtle releases a light-colored mucus that coats all eggs, thus avoiding the impact between one egg and another and maintaining cloaca lubrication, however the characterization of chemical properties and toxicity have not been documented. The objective of this work was to characterize the chemical properties, the toxicity of the substance and to identify the heavy metals present in the cloacal mucus of the sea turtles. From August 2017 to October 2018, manual collection of cloacal mucus was performed, which took place immediately after spawning, with the aid of a 15 ml falcon tube positioned below the cloaca. Ten females were sampled, nine from Eretmochelys imbricata and one from Chelonia mydas, in the coast of Ipojuca / PE. These had a physical chemical aspect with turbidity in some samples, their pH ranged from 8.58 to 9.28 with a tendency to be alkaline, through the mortality numbers of Artemias salina nauplii the mucus showed a variation in its toxicity of very Toxic to slightly toxic, the amount of protein varied in the samples in concentrations from 0.27 mg to 5.17 mg, for heavy metals the highest concentrations were for Aluminum (35.5 mg / L), followed by Iron (28 , 7 mg / L) and Copper with (26.9 mg / L) besides these were also analyzed Manganese (16.1 mg / L), Lead (15.7 mg / L), Nickel (14 mg / L), Cadmium (13.5 mg / L) and Chromium (12.1 mg / L). The analysis showed that mucus is a good biological material for toxicity analysis and metal identification, however there is much to characterize in its chemical composition, requiring the continuity of this work to monitor the animals, as well as to evaluate space-seasonal issues of these metals. Mucus may be a good predictor of animal and environmental health.