01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Síntese, caracterização e estudo citotóxico de um metaloprotótipo à base de rutênio 3+) com potencial atividade antileucêmica(2024-07-29) Moura, Severino Vitor do Nascimento; Silva, Wagner Eduardo da; Belian, Mônica Freire; http://lattes.cnpq.br/2626644337183959; http://lattes.cnpq.br/8612340815622413; http://lattes.cnpq.br/0613129512420118Ruthenium complexes constitute a promising class of metalloprototypes due to their biological activities. These ruthenium-based complexes have peculiar characteristics such as low associated toxicity, effective lability rate and “selectivity” to cancer cells, promoting damage to nuclear nDNA and apoptosis. Using strategies in combination with the Ru3+ ion and ligands with bioactive activities, the complex formed can show enhanced biological responses. This study aimed to synthesize, characterize and study the cytotoxicity of a metalloprototype containing ruthenium and ascorbate as a ligand. The synthetic methodology used enabled the formation of sodium ascorbate from the deprotonation of ascorbic acid with sodium bicarbonate in water, guaranteeing a reaction yield of 83%. The synthesized sodium ascorbate was then reacted with ruthenium (3+) chloride in water for 12 hours, resulting in the formation of the compound with the minimum formula [Ru(Asc)(OH2)4]Cl2 in a yield of 85%. This form was characterized by spectroscopy at infrared wavelengths, with significant differences in the absorption coefficients, such as the C=O and C=C bonds, when compared to ascorbic acid. The [Ru(Asc)(OH2)4]Cl2 complex was characterized by infrared spectroscopy, suggesting its formation through the shifts observed in the absorption bands of the C=O, C=C and C-O bonds. The characterization of the complex by electronic absorption spectroscopy showed hypsochromic shifts of the transitions located in the ligand of the π→π* type and the presence, in the visible, of transitions of the d→d type. The 1H NMR spectrum of the complex showed three signals, the multiplicities of which were two doublets and one triplet, the 13C NMR spectrum showed six signals consistent with the structure of the ligand used and the HSQC NMR spectrum showed three C-H correlation signals, also consistent with the complex studied. The high-resolution mass spectrum of the [Ru(Asc)(OH2)4]Cl2 complex showed six signals for the ruthenium isotopes (98Ru, 99Ru, 100Ru, 101Ru, 102Ru and 104Ru) with the highest relative m/z of 278.75, referring to the presence of Ru3+ and the ascorbate ligand [C6H9O6102Ru]+. The results of the cytotoxicity tests of the [Ru(Asc)(OH2)4]Cl2 complex, carried out on RAW 264.7 (normal cell line), MCF-7 (breast cancer) and HL-60 (human leukemia) cells, showed cytotoxic activity for the HL-60 cell line, with an IC50 of 39.3 μmol.L-1. For the normal cell line, the ruthenium complex proved to be non-cytotoxic (IC50 60.0 μmol.L-1). Therefore, the synthetic strategies used were satisfactory, with yields above 80%. The characterization techniques used were consistent with the formation of the complex obtained. The in vitro toxicological test showed evidence of non-toxicity to healthy cells and proved to be active in human leukemia cells (HL-60), possibly acting as an anti-leukemia candidate.Item PPARs no câncer de fígado: sintenia e interações aplicados a medicina evolutiva(2024-02-26) Leôncio, Thays Maria; Freitas, Nara Suzy Aguiar de; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/6891650997818766; http://lattes.cnpq.br/3551659636270655Liver cancer is the sixth most common diagnosis and the third cause of death. Peroxisome proliferator-activated nuclear receptors (PPARs) are a group of genes that act as transcription factors and have recently been indicated as potential therapeutics for liver cancer. Thus, we studied the PPARs genes associated with their interactions and synteny in an evolutionary medicine approach to liver cancer. The selection of the PPAR family and the primary analysis of their functions were obtained on the NCBI and UniProt platforms. The search for gene interactions was carried out using the protein association network in the STRING database, for the PPAR, PPAR and PPAR subtypes and the synteny analysis of this gene group was carried out. The return of information indicated that the set of genes and gene products evaluated have multiple functionalities of varying magnitude and complexity, being related to liver metabolism and its risk factors or not with liver cancer. In addition to being Mendelian units, they also mainly present positive and negative pleiotropic effects, directly or indirectly involved with liver diseases or healthy phenotypes, such as activities related to thyroid function, spermatogenesis, bone formation and differentiation, satiety control, circadian cycle, among others. These analyzes that are just beginning show promise for an early prognosis involving not only the molecular interactions of PPARs, but their positions in the genome. In this sense, genes are also modulated sometimes to develop their tumorigenic expression, sometimes not, suggesting that their study focused mainly on a healthy phenotype can facilitate preventive actions. This basic approach to evolutionary medicine identified new biomarker targets for hepatocellular carcinoma that could be effectively useful for future genetic evaluations, aiming to better prevent diseases and promote health.Item Análise de incidência de câncer de vesícula biliar em pacientes atendidos no Laboratório Municipal de Saúde Pública do Recife (2014 - 2022)(2024-02-05) Santos, Bruna Maria Correia dos; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/2311174575605049Gallbladder cancer (GBC) is considered a rare type of cancer by the World Health Organization (WHO) and characterized by the absence of symptoms in the early stages, but in advanced stages manifests with abdominal pain. The WHO considers this type of tumor as the most common in the biliary tract, associating it with risk factors such as gallstones, age, female sex, and a higher propensity in white individuals. This study aimed to evaluate the prevalence of gallbladder cancer in patients undergoing elective cholecystectomies, assessing the occurrence of GBC in patients treated at the Municipal Public Health Laboratory of the Recife- Pernambuco from 2014 to 2022. An observational, retrospective, descriptive-analytical study was conducted using secondary sources of patients diagnosed with GBC from 2014 to 2022. A total of 17,970 medical records were analyzed during the study period. Among these, 10 were related to the gallbladder, and only 2 had a diagnosis of GBC. The female sex was present in 70% (7/10) of the cases and 50% were diagnosed with gallstone disease. The most performed operation was simple cholecystectomy. In the two patients with GBC, the diagnosis of gallbladder adenocarcinoma was confirmed. Tumor staging revealed 50% (1/2) at stage IIIb and 50% (1/2) at stage II among the examined cases. Thus, this study confirmed the prevalence of gallbladder adenocarcinoma in GBC and identified cholelithiasis as the main risk factor similar to previous studies.Item Acompanhamento preventivo e agendamento de pacientes com câncer utilizando programação inteira e matroides(2023-04-26) Oliveira, Estéfane Paula Bezerra de; Bocanegra, Silvana; http://lattes.cnpq.br/4596111202208863; http://lattes.cnpq.br/7916017096302322Within the context of the growing number of cancer cases globally, optimization models can help physicians and health professionals to ensure the best use of available resources. This paper presents two constrained optimization models, one for the prioritization of patients for preventive follow-up of cervical cancer and the other for the scheduling of oncology patients in treatment, both using integer programming and AMPL. In addition, a performance analysis of a patient scheduling algorithm using matroids is presented. The models were tested on randomly generated instances, resulting in a list of hypothetical priority patients for performing tests, as well as a list of patients scheduled in their respective shifts.Item Uma breve revisão dos complexos transplatínicos na terapêutica oncológica(2023-03-24) Oliveira, Barbara Daminnicy Nogueira de; Belian, Mônica Freire; Lima, Lidiane Macêdo Alves de; http://lattes.cnpq.br/9825403406472930; http://lattes.cnpq.br/2626644337183959; http://lattes.cnpq.br/4821392524886127Drugs with potential antitumor activity are of great interest to the pharmaceutical industry, since cancer is one of the diseases that affect thousands of people around the world. Cisplatin is a platinum (2+) compound widely used in cancer chemotherapy, with excellent rates of tumor regression, however, the severe side effects and cross-resistance limit its use. Thus, cisplatin analogues compounds such as carboplatin, nedaplatin, and oxaliplatin have been developed and clinically approved. Despite this, patients who use such drugs for treatment may develop cross-resistance. In this context, the need arose to search for new complexes that make it possible to minimize side effects and cross-resistance. Among the new platinum (2+) compounds of interest with antitumor activity studied, there are complexes with trans isomerism, which, due to their different mechanisms of action when binding to nDNA nucleophiles present promising strategies to overcome the mentioned difficulties encountered by current drugs with cis isomerism. Therefore, research on antitumor activity in platinum (2+) complexes with trans isomerism is relevant, to which this work is intended. This study consists of a brief review of the literature, allowing to map examples of transplatin complexes that stand out for their cytotoxic properties, configuring them as promising antineoplastic agents.Item Desenvolvimento de um protótipo antitumoral à base de boro-10 a ser aplicado na terapia de captura de nêutrons(2021-05-26) Sousa, Victor Branco de; Silva, Wagner Eduardo da; http://lattes.cnpq.br/8612340815622413; http://lattes.cnpq.br/4926809415572921In order to develop new prototypes capable of acting as neutron captors, this work presents the synthesis of a system based on polyvinyl alcohol and boric acid for future applications in neutron capture therapy and their characterizations carried out by thermogravimetric analysis, differential scanning of calorimetry, infrared spectroscopy and nuclear magnetic resonance of 1H, 13C and 11B. Two compounds were obtained, reproducible with the proposed methodology, with distinct physical and chemical characteristics, with a pH between 6.6 - 6.8. Thermal analysis data (TGA and DSC) corroborate to confirm the connections made between Boron and the polymer matrix. Boron-11 nuclear magnetic resonance data confirm the formation of three different species of boron, already described in the literature. The performance of the acute toxicity test via the OECD-423 protocol classifies the compound in category 4, showing good agreement for its future application in therapy.Item Cisplatina: uma revisão de literatura(2021-12-23) Pinto, Eddie William Andrews; Belian, Mônica Freire; http://lattes.cnpq.br/2626644337183959; http://lattes.cnpq.br/2493612296858465Cisplatin has been the subject of several studies since its discovery in the 1960s and used as a standard compound in evaluations of the effectiveness of platinum-based chemotherapy treatments. Analyzing the mechanisms of intracellular action of cisplatin, as well as the resistance mechanisms associated with it, is of fundamental importance, since many analogous com-pounds were and are elaborated until today in order to find less adverse effects and resistance to treatment, as well as greater specificity to tumor cells and more effective intracellular toxicity. For this, it is necessary to have knowledge about cisplatin, since its mechanisms of action on DNA is a pattern observed in this compound and its analogues from later generations. With these points in mind, this monograph aimed to identify some of the important productions on cisplatin in the last 5 years through a literature review. The methodology applied for the review consists of a series of steps ranging from the choice of topic to the final writing of the review, being extremely important for a good organization, selection, analysis, interpretation, writing. In all, 46 productions were chosen for a critical analysis. Topics were separated for the interpretation of the results of the analysis of the texts on cisplatin, namely: mechanism of action, adverse effects and new technologies. The 48 productions were organized and separated into topics according to the similar perspectives between the authors and a critical summary and interpretation of these works was performed. As final considerations, the review highlighted the importance of gathering such texts on cisplatin for study and instructions on the directions and trends of new technologies, such as the use of nanoemulsions and nanocarriers for cisplatin with the objective of increasing the efficiency of chemotherapy based on this compound and reduce the side effects associated with it.Item Avaliação do potencial antitumoral in vitro e da atividade imunomoduladora do composto mesoiônico 5-(4-clorofenil)-3-metil-4-fenil-1,3-tiazólio-2-tiolato (MI-2)(2020-11-04) Andrade, Bárbara Fernanda Pessoa de; Silva, Elayne Cristine Soares da; Andrade, Jeyce Kelle Ferreira de; http://lattes.cnpq.br/8970644656035762; http://lattes.cnpq.br/2064359935012193; http://lattes.cnpq.br/8580695386267290Cancer is classified by the World Health Organization (WHO) as a group of diseases, wich can develop anywhere in the body, from the uncontrolled grownth of genetically altered cells and wich have the ability to invade adjacent tissues and can therefore, if install simultaneously in more than one organ. It is considered by WHO as the second leading cause of death in the world, killing about 9.6 million people. In Brazil, according to the National Cancer Institute (INCA), it is estimated that in 2020 309,750 new cases of cancer will be registered for men and about 316,280 new cases for women. Cancer patients are usually treated with chemotherapy, radiation therapy, surgical intervention and in some cases, immunotherapy. In view of the high cost and the remarkable toxicity presented by the vast majority of treatments currently available, such as weight loss and hair, in addition to anemia, leukopenia, thrombocytopenia and immunosuppression that make them extremely vulnerable; the search for new therapeutic possibilities is necessary to increase the chances of cure and quality of life. Mesoionic compounds are a group of substances defined as heterocyclic betaines, mostly of synthetic origin, biologically active and wich have been widely studied for their chemical characteristics and diverse functions, among which the antitumor potential, which has been the object of prominence, stands out of numerous surveys. The compound used in the present work (MI-2) is one of the derivatives of the thiadiazole class mesoionics called 5-(4-chlorophenyl)-3-methyl-4-phenyl-1,3-thiazolium-2-thiolate. The objective of this work was to analyze the antitumor potential of this compound, evaluating the cytotoxicity in tumor cell lines, morphological characteristics of these cells, DNA fragmentation; in addition to the analysis of cytotoxicity in cells of the imune system of rats (Rattus novergicus). The results presented so far, demonstrate satisfactory responses in antitumor evaluation tests, especially for leukemias; and a probable low toxicity in isolated rat lymphocytes. This compound is part of an ongoing research project.Item Efeito da lectina de folhas de Schinus terebinthifolia sobre a formação de vasos do saco vitelínico e morfologia de embriões de Coturnix japônica(2019-08-13) França, Rayssa Perla Martins; Pontual, Emmanuel Viana; http://lattes.cnpq.br/1777060469196142; http://lattes.cnpq.br/4999755464041290Angiogenesis corresponds to the budding of new blood vessels starting from other preexisting ones. This process is of great importance for development of tumor cells. Schinus terebinthifolia (Brazilian Peppertree) leaves express a thermostable and antimicrobial lectin (SteLL). This work reports the effect of SteLL on the angiogenesis process using the in vivo model of yolk sac membrane of Coturnix japonica (Japanese quail) embryos. The fractal analysis by box counting and information dimension indicated that SteLL at 1.35 mg/mL reduced the angiogenesis of C. japonica yolk sac membrane. The inhibition of the vascular network formation in the yolk sac membrane resulted in decreased blood supply to the embryos, and consequently area, perimeter and percentage of cephalic length of embryos were significantly reduced in the group treated with SteLL, corroborating with the antiangiogenic activity. In conclusion, SteLL is an antiangiogenic agent and this result stimulate future investigations about its antitumor activity.Item Relatório de Estágio Supervisionado Obrigatório (ESO): acompanhamento da rotina clínico-cirúrgica na Clínica CORe - Oncologia Veterinária(2020-12-16) Ferreira, Maria Islane Araújo; Silva, Ellen Cordeiro Bento da; http://lattes.cnpq.br/4626343121504409; http://lattes.cnpq.br/7416766519843144The Mandatory Supervised Internship (ESO) of the undergraduate course in Bachelor of Veterinary Medicine, by the Federal Rural University of Pernambuco (UFRPE), was carried out under the guidance of Profª. Drª. Ellen Cordeiro Bento da Silva, by student Maria Islane Araújo Ferreira. The same was carried out at the Veterinary Clinic CORe - Veterinary Oncology, located in the neighborhood of Rosarinho, Recife - PE, from August 17, 2020 to October 30, 2020, under the supervision of Veterinary Oncologist Maria Cecília Oliveira Nascimento, totaling 420 hours. Thus, this report, corresponding to the Course Conclusion Work, includes the description of the internship location, characterizing the physical structure, personnel, the infrastructure of the internship location and its functioning, description of the activities carried out and the clinical cases followed, as well as an article in the case report format. The latter consists of the description of an experience lived during the internship, corresponding to a carcinoma in the thyroid gland, which was given greater focus in the report. From the above, it can be seen that ESO is an indispensable activity for the training of undergraduate students, which allows the professional on the verge to confirm or modify the desired area of practice, through practical experience, so that for the case there was the confirmation of the desire to work with a veterinary oncologist. In turn, in relation to the accompanied case, it is concluded that theireoidectomy is the treatment of choice for thyroid carcinoma, despite the risk of metastasis and hypothyroidism.