01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Item Achados epidemiológicos e comparação dos achados laboratoriais de cães com infecção do trato urinário provocada por bactérias Gram-positivas e Gram-negativas(2024-02-22) Oliveira, Angélica Prado de; Guimarães, Janaina Azevedo; http://lattes.cnpq.br/8177426224558979; http://lattes.cnpq.br/9073621897539009Item Análise de mecanismos de resistência a polimixina em isolados de Klebsiella pneumoniae(2021-12-17) Andrade, Rauana Lins de; Almeida, Anna Carolina Soares; Santos, Bárbara Nazly Rodrigues; http://lattes.cnpq.br/4795529090461229; http://lattes.cnpq.br/4891800920829895; http://lattes.cnpq.br/8836137133949593Infections for Klebsiella pneumoniae Carbapenemase-producing have been a public health problem leading to increased patient morbidity and mortality, which has led to the reintroduction of a previously discontinued antimicrobial for human use, polymyxins. The raise in resistance to polymyxins has made treatment even more difficult, which is worrying due to the high worldwide dissemination of these strains.This study aimed to perform a genetic analysis of two-component systems involved in polymyxin resistance in Klebsiella pneumoniae isolates. Sixteen multiresistant K. pneumoniae isolates were studied. The clonal relationship was performed from the investigation of repeated extragenic palindromic sequences (REP). The genes of the two-component enzyme regulatory systems (pmrA, pmrB, phoP, phoQ) were amplified by polymerase chain reaction (PCR). The identification of mutations was performed by DNA sequencing with comparative analysis using the MGH 78578 strain as reference. It was possible to distinguish the presence of four groups with clonal relationship of the same species with a variation of 2 to 5 unshared bands, indicating a pattern of similarity between all bacteria in the study. All isolates had more than one mutation in the coding regions of the genes studied, the prevalence was of mutations classified as silent in pmrA and phoP. However, Frameshift Nonsense and Missense mutations were identified in pmrB and phoQ genes, which led to alterations in the amino acid chain and production of a non-functional protein. Nucleotide alterations in the coding regions of the TCS regulatory genes (phoPQ and pmrAB) and the compromise of the protein sequence are considered the most relevant mechanisms regarding the mediation of resistance to polymyxins.Item Relatório de Estágio Supervisionado Obrigatório realizado no Parque Estadual Dois Irmãos (PEDI), Recife - PE. Doença ulcerativa septicêmica cutânea (SCUD) em cágado-de-barbicha (Phrynops geoffroanus)(2022-10-04) Tiné, Marcella Ribeiro; Cavalcanti, Erika Fernanda Torres Samico Fernandes; http://lattes.cnpq.br/5256493441853885; http://lattes.cnpq.br/9234768668750061The objective of this work is to report the experiences of student Marcella Ribeiro Tiné, held at Dois Irmãos State Park, which were developed from June 28 to September 12, 2022 at Dois Irmãos State Park (PEDI), from 8 am to 5 pm, totaling 420 hours of internship, carried out in 54 days. The internship was developed in the field of wild animal medicine, under the supervision of Dr. Márcio André da Silva and guidance from Prof. Erika Fernanda Torres Samico Fernandes Cavalcanti. The first part of the work reports the activities carried out in the internship during the mentioned period, while the second part deals with a case report entitled “Septicemic Cutaneous Ulcerative Disease (SCUD) in Bearded Tortoise (Phrynops geoffroanus)”, a theme chosen based on its importance. and connection with said stage.