01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Efeitos da elicitação isolada e combinada de ácido salicílico e metil jasmonato nos parâmetros biométricos e bioquímicos em Lippia alba (Verbenaceae)(2024-09-27) Paula, Maria Eduarda Magalhães de; Sperandio, Marcus Vinícius Loss; http://lattes.cnpq.br/4157291425794314; http://lattes.cnpq.br/8710784594902570Lippia alba is an aromatic shrub from the Verbenaceae family, primarily associated with Brazil's Caatinga and Cerrado regions. It is used in traditional medicine for its pharmacological properties, those being attributed to the essential oil the plant produces as a defense mechanism. A tool that can be used to increase the productivity of bioactive compounds in both in vitro and in vivo development is elicitation. This study aims to analyze the effects of isolated and combined applications of the elicitors Methyl Jasmonate and Salicylic Acid on L. alba, using biochemical and biometric data as parameters one week after the elicitation. Cuttings of L. alba were maintained in hydroponic cultivation and later transferred to a greenhouse. The combined and isolated treatments received a concentration of 4 mM Salicylic Acid and 2 mM Methyl Jasmonate. An additional group was established, receiving an application of 1% ethanol. The experiment was harvested one week after the elicitation. Elicited treatments showed significant differences in essential oil yield, oxidative stress analysis, and enzymatic activity compared to the control. Growth parameters, along with carbohydrate and protein levels, kept similar patterns in all treatments. In vivo, combined Salicylic Acid and Methyl Jasmonate elicitation increased essential oil yield on L. alba without affecting biomass production, suggesting an efficient modulation of the plant metabolism. Therefore, elicitation at the tested concentrations demonstrated to be a viable tool to optimize the production of bioactive compounds in this species in a short elicitation period.Item Qualidade de queijos coalhos adicionados de óleos essenciais em sua formulação(2024-09-30) Silva, Maria Manuele Dantas Pereira da; Soares, Luciana Felizardo Pereira; Rocha, Laura Leandro da; http://lattes.cnpq.br/7226123934022759; http://lattes.cnpq.br/4071178363761831; http://lattes.cnpq.br/9024775534481122The objective of this study was to evaluate the physicochemical composition of coalho cheese with the addition of essential oils of oregano, rosemary, and thyme in its formulation at different maturation times. The research was designed as a completely randomized design in a 3x4 factorial arrangement, in triplicate, at three maturation times: 0 (fresh cheese), 15, and 30 days, with four types of coalho cheese: control cheese without essential oil; with oregano essential oil; rosemary oil; and thyme oil, the last three with equivalent concentrations of 40 μL. The physicochemical characteristics of the cheeses at each period were evaluated. The quality of the milk used as raw material was assessed for physical properties, chemical composition, and the presence of pathogenic microorganisms. The milk used as raw material met the quality standards required by legislation. Compared to fresh cheese, a decrease in moisture content was observed over the maturation period, from 55.94% moisture at 0 days to 54.62% and 53.58% at 15 and 30 days, respectively. Regarding the percentage of fat, ash, and acidity, there was a reduction over the evaluated times. There was no significant variation in protein content between the maturation periods. According to the average yields of the treatments (kg), they corresponded to 1.41 kg, 1.30 kg, 1.51 kg, and 1.43 kg for cheeses without oils, with oregano oil, rosemary oil, and thyme oil, respectively. Greater economic viability was observed in cheeses with rosemary and thyme oils, with percentages of 19.5% and 2.9% more favorable compared to the control. The use of essential oils of oregano, rosemary, and thyme in the formulation of coalho cheese proved to be a promising strategy that can modify and improve specific characteristics of coalho cheese, depending on the desired objective. Additionally, it can increase the yield of cheeses with the addition of rosemary and thyme oils, offering a viable and advantageous alternative for the dairy industry and providing differentiated products with higher added value for the consumer market.Item Análise da composição química do óleo essencial de espécies de Plectranthus cultivadas sob condições de estresse e avaliação Do seu potencial acaricida sobre o Tetranychus urticae(2024-07-31) Silva, Beatriz Steffanie Gomes da; Moraes, Marcílio Martins de; http://lattes.cnpq.br/6957579091162269; http://lattes.cnpq.br/4696332206790980Plants are living organisms capable of producing secondary metabolites as a defense and development mechanism. What stimulates the activation of secondary metabolism are changes in the natural conditions necessary for the survival of the plant, called biotic or abiotic stresses. Essential oils (EOs) are complex mixtures of constituents originating from the secondary metabolism of plants, and have different biological properties, from medicinal purposes to pest control. Thus, the objective of this study was to investigate the variation in the chemical composition of EOs from two species of the genus Plectranthus (P. barbatus and P. ornatus) under controlled stress conditions and the antioxidant and acaricidal potential against Tetranychus urticae (spotted spider mite). The species were separated into groups and subjected to different stress conditions: manual predation (PM), methyl salicylate (SM), methyl jasmonate (MJ), saline (S), water scarcity (E), ultraviolet radiation (UV), and the group without any stress (control). The EOs were obtained by hydrodistillation and the chemical composition was determined by gas chromatography coupled to mass spectrum (GC-MS). The data were subjected to multivariate analyses (PCA, heatmap and molecular network). The acaricidal activity was evaluated comparatively through the LC90 of the positive control Azamax and the antioxidant activity by the ABTS.+ and DPPH methods. For both species, the chemical composition identified in the control groups showed a predominance of constituents derived from sesquiterpenes, with (E)- caryophyllene as the major constituent. However, in the stressed groups, monoterpenes were present in higher percentages, significantly reducing the percentage of the major compound of the control. This variation was confirmed by all multivariate analyses used. The EOs of both species showed low acaricidal activity against the two-spotted spider mite, with mortality rates equal to 12.00% (P. barbatus) and 22.72% (P. ornatus). P. barbatus showed better antioxidant activity for ABTS.+ (62.74 μg/ml) while P. ornatus showed better for DPPH. (163.5 μg/ml). However, compared to the positive controls Trolox (4.13 μg/ml) and ascorbic acid (1.62 μg/ml), respectively, the activities presented by both species were relatively low. Therefore, it was possible to verify that the EOs of these species present antioxidant and acaricidal activities, although low, and that different stress conditions alter the chemical composition of the plant.Item Composição química, atividade acaricida, propriedade antioxidante, inibição da acetilcolinesterase e toxicidade do óleo essencial das folhas de Sparattanthelium botocudorum(2024-03-05) Silva, Mirian Luzinete da; Camara, Cláudio Augusto Gomes da; http://lattes.cnpq.br/5615678215435460; http://lattes.cnpq.br/4499498152298785Sparattanthelium botocudorum is a species endemic to Brazil, commonly known as “Canela-brava”, this shrub is distributed on the coast of Paraíba and in the state of Pernambuco. Due to the scarcity of chemical and biological studies on this plant, the research aimed to determine the chemical composition of the essential oil of the leaves as well as the evaluation of the acaricidal, antioxidant and toxicity properties against Artemia salina. The essential oil, extracted from fresh leaves, through the hydrodistillation technique with the aid of a Clevenger-type device, had a yield of 0.40% m/m and its chemical characterization, through Gas Chromatography coupled to the Mass Spectrometer (CG-MS), emerged as the majority constituents were the sesquiterpenes Germanecrene D with (38.28 %), E-Nerolidol (18.95 %), [beta]-Caryophyllene (14.49 %) and Biciclogermacrene (14.18 %) and the single monoterpene Z-[beta]-Ocimene identified in the OE with a percentage of 0.37%. Residual contact bioassays were carried out, with the aim of identifying the effects of the oil on the two-spotted mite, and showed promising toxicity. The residual contact bioassay, in which the mites are arranged on already treated leaves, showed LC50 = 16.5 μL/mL. Furthermore, the oil also demonstrated ovicidal properties with an estimated LC50 of 1.20 μL/mL. And regarding repellency activity, the oil showed a better potential with the LC30 concentration 8.8 μL/mL. After evaluating the inhibition activity of the acetylcholinesterase enzyme, the essential oil from the leaves of S. botocudorum inhibited the enzyme, since the LC50 resulted was 2.9 μg/mL below the LC50 of the positive control, which is 5.95 μg/mL. mL. Furthermore, OE presented antioxidant properties for the free radical DPPH˙, estimating an EC50 = 110.3 μg/mL, while for the free radical ABTS˙+ it was EC50 = 49.23 μg/mL. Finally, the oil exhibited a toxicity concentration of 700 μg/mL for the toxicity test against A. salina, which compared to what described in the literature had no toxicity. Therefore, the study showed that the essential oil presented similar and promising effects compared to already commercialized acaricides, and that the bioassays presented different routes of action, therefore acting with different mechanisms on the pest and presenting a biological property of antioxidant activity.Item Atividade antimicrobiana de óleos essenciais de orégano (Origanum vulgare), pimenta rosa (Schinus terebinthifolia) e melaleuca (Melaleuca alternifólia) frente aos patógenos de origem alimentar(2024-02-21) Silva, Esterfani Pereira da; Soares, Luciana Felizardo Pereira; http://lattes.cnpq.br/4071178363761831; http://lattes.cnpq.br/3144149210017027In recent years, the use of synthetic additives to prevent food spoilage by microorganisms has been a worrying factor for the food industry, given their negative impact on consumer health. In order to overcome this impasse, essential oils have emerged as an alternative, containing compounds and active principles that inhibit the development of microorganisms. The aim of this study was to evaluate the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the commercially obtained essential oils of Tea tree (Melaleuca alternifolia), Oregano (Origanum vulgare) and Pink pepper (Schinus terebinthifolia). For the study, bacterial isolates at a concentration of 3.5 x 107 CFU/ml (0.5 on the Mc Farland scale) of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) obtained commercially from the American Type Culture Collection were used. The incubated plates were visually analyzed to determine the minimum inhibitory concentration (MIC). In the wells where inhibition of bacterial growth was observed, 20 μL of the suspensions were collected and sown on Mueller-Hinton agar plates in an oven at 37ºC for 24 hours to analyze bacterial growth and determine the minimum bactericidal concentration (MBC). The mean MIC values for oregano, pink pepper and tea tree essential oils were 2500 μg/ml, 650 μg/ml and 500 μg/ml for the E. coli strains, respectively, while the minimum inhibitory concentration of the treatments was 500 μg/ml for the S. aureus bacterial group. As far as CBM is concerned, the average concentrations for the essential oils of oregano, pink pepper and tea tree were 2500 μg/ml, 625 μg/ml and 5000 μg/ml for the E. coli strains and 1250 μg/ml, 2500 μg/ml and 625 μg/ml for the gram-positive bacteria S. aureus, respectively.Item Monitoramento tecnológico de nanoemulsões produzidas a partir de óleos essenciais e sua aplicação na saúde humana e veterinária(2021-12-09) Bastos, Paulo Eduardo da Silva; Cadena, Marilia Ribeiro Sales; http://lattes.cnpq.br/6434031656742674; http://lattes.cnpq.br/0631591157585136Nanoemulsions produced with essential oils (NEOEs) are formulations with great therapeutic potential within human and veterinary health, and can be applied for the most diverse purposes. The present work aimed at technological monitoring, raising patents and articles referring to NEOEs with applicability in the field of human and veterinary health. The documents were obtained through a systematic review, in several databases and went through inclusion and exclusion criteria, and then were grouped for the analysis of variables, such as: Type of documents by country; Publication timeline by type of document; Application area; Applicability of NEOEs; among others. 153 articles and 34 patents were found. It was observed that Brazil is the country with the highest number of publications for both types of documents. For articles, along with Brazil, we observe that India and Iran have a large amount of publication, but they have a low amount of patent publication. This indicates that these countries are not protecting their products, reducing the inventive potential of their respective nations. When we evaluate patents, Brazil remains in first place, but now alongside it, we have China and the United States, that is, these last two countries protect their ideas more than they publish scientific articles. Analyzing the applicability of NEOEs, there is a greater exploration of their cytotoxic potential, that is, being applied to the destruction of pathogenic cells (Bacteria, fungi, protozoa, worms and cancer cells). Most of the patents found belonged to educational institutions, and few to private industry. In the world, little attention has been given to the development of NEOE with application in veterinary medicine, which makes it an unexplored field, but with great potential as it is a high monetary power industry. Although there is a greater amount of products aimed at human health, few systemic diseases have been explored for the application of NEOEs, which would be another promising field for the development of future research.Item Produção de óleos essenciais a partir de plantas cultivadas com efluentes domésticos(2019) Secundino, Jadde Milena Guedes; Leite, Wanderli Rogério Moreira; http://lattes.cnpq.br/9563775970871734; http://lattes.cnpq.br/5149117075299567Item Composição química, atividade inseticida e potencial antioxidante dos óleos essenciais das folhas e flores de Plumeria pudica Jacq(2022-09-30) Arcelino, Ariadna Isis Oliveira de; Moraes, Marcílio Martins de; http://lattes.cnpq.br/0321382004671685; http://lattes.cnpq.br/0321382004671685Agricultural producers usually suffer from the attack of pest insects that cause great damage to plantations, among these insects is Plutella xylostella, popularly known as cruciferous moth, as it usually attacks Brassicas plantations, this pest has already caused a global damage of about of US$ 4 billion. Currently, the control of this pest is carried out with commercial insecticides, but the excessive use of these products tends to cause greater resistance of the pest, in addition to contaminating soil, rural workers and mammals. With that, seeing being studied the use of natural products such as extract and essential oil of plants, as an alternative for the control of these agricultural pests, since these materials have in their chemical composition secondary metabolites with high toxicity to insect pests without causing damage to the environment, workers and consumers. The present work analyzed the chemical composition of the essential oil of the leaves and flowers of Plumeria pudica in order to evaluate its chemical composition and insecticidal and antioxidant properties. The analysis by gas chromatography coupled with mass spectrometry (GC-MS) revealed the presence of 8 and 13 compounds in the essential oil of leaves and flowers, respectively. The major compounds identified were (2E)-hexenal (40.67%) and lauric acid (39.97%) for leaves and flowers, respectively. The toxicity bioassay was carried out through residual contact and direct contact with the oil of P. pudica flowers with concentrations of 10μL/mL and 15μL/mL on P. xylostella, for the test using residual contact the oil did not show toxicity, on the other hand, using direct contact, the concentration of 10μL/mL showed a toxicity of 92% and 15μL/mL of 93.10%. In the antioxidant tests it was found that the two oils were active both in the ABTS and DPPH test. In the antioxidant tests, it was found that the oils from the leaves and flowers were active both in the ABTS test and with DPPH. For the acetylcholinesterase (AChE) inhibition test, the oils from the leaves and flowers of P. pudica were used, both oils showed inhibition, and the flower oil showed the closest inhibition to the positive control with an IC50 of 9 .94 mg/ml. The cytotoxicity assay against Artemia salina was also performed with the oils of leaves and flowers with concentrations between 10-100μg/mL and both showed no toxicity.Item Composição química, atividade citotóxica, acaricida e antioxidante do óleo essencial das flores de Caesalpinia echinata (Pau-brasil)(2021-12-10) Rodrigues, Lucas Vitor Batista; Camara, Cláudio Augusto Gomes da; http://lattes.cnpq.br/5615678215435460; http://lattes.cnpq.br/7934872766687903Belonging to the family Fabaceae, Caesalpinia echinata is a tree endemic to Brazil, popularly known as pau-brasil. It was widely explored because of the quality of its wood and the produc-tion of a red pigment and much coveted in colonial times for dyeing fabrics and due to predatory extractivism, it was almost extinct. Currently, wood is much sought after for the manufacture of bows for violins for its ideal stiffness and density. The scarcity of chemical and biological studies of different parts of the plant motivated the chemical composition of the essential oil of the flowers as well as the evaluation of the acaricide, cytotoxicity properties against saline ar-temia and antioxidant. The flowers of C. echinata were collected on the campus of the Federal Rural University of Pernambuco. The essential oil (EO) was extracted from the hydrodistilla-tion technique by a modified Clevenger type apparatus. The chemical composition of the oil was determined by gas chromatography coupled to a mass spectrometer. Residual contact was the method selected to evaluate the acaricide action on Tetranychus urticae and the biological model selected for cytotoxicity evaluation was That of A. salina. The EO of the flowers pre-sented monoterpenes as the main chemical class. The main compounds identified were linalool (32.33%), Z-jasmona (14.94%) and [beta]-E-ocimene (10.44%). The essential oil for the control of T. urticae presented a LC50 = 290.15 uL/mL. The EO revealed an antioxidant capacity for the free radical DPPH˙ estimated at EC50 = 364.10 ug/mL, while for the free radical ABTS˙+ was EC50 = 280.50 ug/mL. The oil exhibited a LC50 = 97.41ug/mL for the toxicity test against A. salina. This study showed that the essential oil of C. echinata flowers has acaricide, antioxidant and cytotoxic biological property against A. salina.Item Identificação química e atividade antimicrobiana de óleos essenciais das folhas de Piper marginatum obtidos em meio ácido, básico e neutro(2019-12-18) Claudino, Líbna Larissa Monteiro; Ramos, Clécio Souza; http://lattes.cnpq.br/3782004073843685; http://lattes.cnpq.br/1342247970543261The increasing resistance of pathogenic microorganisms to conventional antimicrobials has been faced as a worldwide public health problem. The generalized use of antimicrobials in the treatment of infections caused by pathogens indulges microbial resistance, rendering them ineffective. The search for new plant compounds becomes an innovative alternative as an excellent source of new bioactive molecules. This work aims to define the chemical composition, as well as to evaluate the antimicrobial activity of essential oils of Piper marginatum leaves in basic, acid and neutral environments. The collection of plant material was made in the reserve dois irmãos located in the city of Recife on the campus of the Universidade Federal Rural de Pernambuco (UFRPE). The essential oils extracted from Piper marginatum leaves were obtained by hydrodistillation technique, using Clevenger type apparatus and the identification of their chemical constituents were determined by GC-MS. To obtain the oil in acidic environments an acidic solution of sulfuric acid: nitric acid in the molar ratio (1: 1) was prepared, as well as to obtain in basic environments a basic solution of potassium hydroxide was prepared at a concentration of 5 Mol.L-1. Antimicrobial activity was evaluated using the MIC method (Minimum Inhibitory Concentration). The results showed that the oil obtained in acid environments had a better activity against gram-negative E. coli bacteria with a MIC of 78.1 μg/ml and the fungi C. Albicans and C. Utilis with a MIC of 312 μg/ml and 78.1 μg/ml respectively. The activities of oils in basic and neutral environments were considered moderate to weak for all microorganisms tested, and showed MIC ranging from 625 μg/ml to 2500 μg/ml for both oils. Regarding the identification of the constituents, the major compounds found in the oils obtained in the basic and neutral environments were Macrocarpene, and Z Asarone, respectively. These results contribute to the chemical and biological knowledge of P. Marginatum, which is widely publicized as an excellent plant for therapeutic purposes.