01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Germinação de sementes de Moringa oleifera Lam. sob diferentes concentrações de cloreto de potássio(2024-03-08) Souza, Danilo Nunes Lemos Santos de; Lima, Elifábia Neves de; http://lattes.cnpq.br/6117763430610324; http://lattes.cnpq.br/3607823032961358This study investigates the impact of salt stress on the germination and initial growth of Moringa oleifera LAM., a plant recognized for its nutritional and medicinal properties. The introduction contextualizes the importance of Moringa oleifera and highlights the relevance of studying the effects of saline stress in agricultural environments affected by soil salinization. The experiment evaluated the response of seedlings subjected to saline stress, comparing seeds presoaked in water for 24 hours with those not soaked. Carried out under controlled laboratory conditions, the experiment lasted 15 days, with seeds subjected to different saline solution treatments with Kcl. The concentrations used were Kcl 0.04 M, Kcl 0.08 M and Kcl 0.12 M. The variables analyzed included germination percentage, germination speed index, hypocotyl and main root length. The results indicated that saline stress negatively impacts germination and growth, with a significant reduction in treatments with higher saline concentrations. This study reinforces the importance of understanding the resistance mechanisms of Moringa oleifera to saline stress for the development of sustainable agricultural practices in areas affected by salinity. Contributes to scientific knowledge about plant tolerance to adverse conditions, guiding future research to improve crop resilience in saline environments.Item Efeito do estresse salino na germinação de sementes da Moringa oleifera Lam(2023-05-12) Alves, Beatriz Silva; Lima, Elifábia Neves de; http://lattes.cnpq.br/6117763430610324; http://lattes.cnpq.br/4640617602930699La Moringa oleifera Lam. es un árbol originario de la India y se cultiva en vários países por su multifuncionalidad. Actualmente, se encuentra ampliamente en el Nordeste brasileño y su crecimiento en esta región está siendo utilizado como purificador de agua en áreas rurales. Dada su gran importancia para los agricultores familiares por sus múltiples aplicaciones de uso, como fuente de alimento, producción de jabón y biodiesel, como sustituto proteico y en su aplicación farmacológica. Este trabajo tuvo como objetivo evaluar el efecto del estrés salino em la germinación de semillas de moringa expuestas a diferentes sales y niveles de salinidad, con el fin de obtener una productividad óptima para los agricultores, ya que la región semiárida se caracteriza por altas concentraciones de sales en el suelo, debido a la escasez de lluvias y la alta tasa de evaporación. El experimento fue realizado en el Laboratorio de Ecología Vegetal de los Ecosistemas del Nordeste (LEVEN) - UFRPE, ubicado en la ciudad de Recife. El diseño experimental fue completamente al azar, con siete tratamientos y cuatro repeticiones, 100 semillas por cada tratamiento. Las sales utilizadas fueron NaCl y KCl con los siguientes niveles: 0,04 M, 0,08 M y 0,12 M. Las variables analizadas fueron el porcentaje de germinación (G%), índice de velocidad de germinación (IVG), longitud del hipocótilo y raíz y la masa seca de las plántulas. Se concluyó que la salinidad no afectó significativamente el porcentaje de germinación de Moringa oleifera Lam. para lãs concentraciones de NaCl y KCl, sin embargo, hubo una reducción en el índice de velocidad de germinación con el aumento de los niveles salinos en las condiciones establecidas en este trabajo. Se observó una reducción en la longitud del hipocótilo y raíz con el aumento de la concentración de sales, siendo la longitud de las plântulas y el número de plántulas normales las más afectadas por el NaCl. La influencia Del KCl aún no ha sido concluyente con este trabajo, siendo necesario seguir trabajando.Item Sistemas eletroquímicos para tratar amostras de água contendo corante têxtil como ferramenta didática para divulgação da poluição hídrica(2021-07-07) Neves, Eloisa Ferreira; Freitas, Kátia Cristina Silva de; http://lattes.cnpq.br/5859266863241551; http://lattes.cnpq.br/9764373408949634Didactic workshops for scientific and technological dissemination, in addition to contributing to motivational aspects for the development of learning, are an interactive strategy that can provide the development of science learning. This research aims to use electrochemical systems to characterize the physicochemical properties of the coagulant lectin from Moringa oleifera seeds (cMoL) in the treatment of water samples containing indigo-type textile dyes as teaching tools for scientific dissemination about water pollution in effluents from the textile industry. The methodological development of this research comprises the development of aluminum piles using aluminum cans and PET bottles for the treatment of indigo-type dye solution; the determination of electrochemical potentials and cyclic voltammograms generated by the agglutination of cMoL immobilized on the MOF with an aqueous solution of indigo carmine dye; the development of potentiometric biosensors for the determination of indigo dye residues in aqueous media. Considerable differences were observed in the electrochemical potentials in relation to the platinum electrode (Pt), MOF coated platinum working electrode (Pt/MOF), MOF coated platinum electrode and immobilized cMoL (Pt/MOF/cMoL) and when interacting Pt /MOF/cMoL with different concentrations of galactose. Cyclic voltammetry showed results with significant differences between the untreated dye sample and the dye samples after treatment. The development of didactic workshops in school spaces to validate the electrochemical system as a didactic tool applied to learning and dissemination about water pollution caused by the disposal of indigo dyes in textile industry effluents is relevant for the construction of concepts related to electrochemistry.Item Potencial biotecnológico do inibidor de tripsina de flores de Moringa oleifera: uma revisão(2021-12-16) Medeiros, Êsdras da Silva; Pontual, Emmanuel Viana; http://lattes.cnpq.br/1777060469196142; http://lattes.cnpq.br/8379001129790219This work provides a review on the applications of the Moringa oleifera flower trypsin inhibitor (MoFTI) as an insecticidal, antiparasitic, antitumor, immunomodulatory and anti-inflammatory agent. MoFTI is a 18.2-kDa protein (Ki against trypsin of 2.4 μM) that killed (LC50 of 0.3 mg/mL) or delayed the development of newly hatched Aedes aegypti larvae and impaired the growth and viability of the larval gut microbiota (MIC of 0.031 mg/mL; MBC of 1.0 mg/mL). It was also reported that MoFTI caused lysis of Trypanosoma cruzi trypomastigotes (LC50 of 41.2 μg/mL). The inhibitor stimulated the release of the inflammatory cytokines TNF-α and INF-γ, the anti-inflammatory cytokine IL-10, and NO by T. cruzi-infected human peripheral blood mononuclear cells (PBMCs) with the advantage of exerts low toxicity to murine peritoneal macrophages and Vero cells, and no toxicity to human PBMCs. It is known that MoFTI antitumor activity involves the reduction of sarcoma 180 tumor weight in mice and the impairment of the vascularization in tumor microenvironment without exerting toxicity to the animals. Also the inhibitor is an anti-inflammatory and immunomodulatory agent because it reduced paddle edema and leukocyte migration to the peritoneal cavity in mice, and modulated levels of NO, pro-inflammatory (TNF-α, IL-6 and IL-17A) and anti-inflammatory (IL-4 and IL-10) cytokines, respectively. The reviewed data implicate MoFTI as an interesting molecule for biotechnological applications, whether due to its insecticidal potential or pharmacological value.Item Investigação do efeito do extrato de flores de Moringa oleifera Lamarck (Moringaceae) na sobrevivência e atividade de enzimas digestivas de larvas de Plutella xylostella Linnaeus (Lepidoptera: Plutellidae)(2019-02-01) Ferreira, Camila Azevedo; Pontual, Emmanuel Viana; Almeida, Welton Aaron de; http://lattes.cnpq.br/6880632191779221; http://lattes.cnpq.br/1777060469196142; http://lattes.cnpq.br/1108068314774367Plutella xylostella is a multiresistant agricultural pest that attacks brassica plantations in Brazil and worldwide. The use of natural products with insecticidal properties to control P. xylostella may be advantageous due to the higher degree of degradability and lower persistence of these compounds in the environment when compared with the effects of synthetic insecticides. Moringa oleifera Lam. (Moringaceae) is a pantropical tree that synthesizes proteins (lectins and trypsin inhibitor) whose insecticidal activity has been reported. In the present work, the M. oleifera flower extract containing trypsin inhibitor (MoFTI, M. oleifera flower trypsin inhibitor) was investigated for the presence of peptides resistant to hydrolysis by digestive proteases and effect on survival and protease activity from midgut of P. xylostella third instar larvae (L3). The flower extract showed a high molecular weight polypeptide, which was resistant to hydrolysis by larval gut proteases up to 270 min incubation, revealed by polyacrylamide gel electrophoresis. The larvae were sensitive to the treatment with the extract and the mortality curve revealed a dose dependent response following a polynomial order 2 regression with R2 = 0.99. The maximum mortality rate (60 ± 10%) of larvae was obtained for the extract at 2% (w/v). The adults who emerged from treatment with flower extract showed morphological alterations, including atrophy of the wings and inability to move. In addition, some insects were not able to abandon the cocoon after the end of the pupal phase. The total protease and trypsin-like activities from larval gut were reduced in 67.75 ± 0.64% and 65.83 ± 6.3% by the flower extract at 0.8% and 1%, respectively. Deterrent alimentary effect was not detected when the larvae were reared with cabbage discs treated with the extract at 0.5 and 1%. In conclusion, the M. oleifera flower extract is a larvicidal agent against P. xylostella by cause larval mortality and malformations in adult insects. The larvicidal effect of the flower extract may be linked to the inhibition of enzymes important for the digestive processes of P. xylostella.Item Uso de semente de Moringa oleífera no tratamento de água de ambiente estuarino(2021-12-07) Sobral, Amanda Mirelly Santos; Shinohara, Neide Kazue Sakugawa; http://lattes.cnpq.br/7105928729564845; http://lattes.cnpq.br/6807220814014834Moringa oleifera is a plant native to India, traditionally used to treat wastewater and drinking water, aiming to remove turbidity due to its ability to have cationic proteins in its seed, which are soluble in water and can adsorb suspended particles and the accompanying microbiota. The objective was to analyze the use of Moringa oleifera as a natural coagulant and antimicrobial agent for use as primary water treatment for the Araçá lagoon, located in the city of Recife/PE, classified as an estuarine environment, in a densely populated region with intense discharge of domestic and industrial effluents. Physical-chemical tests were performed (pH, turbidity and color) and quantification of total and thermotolerant coliforms in the raw water and after treatment with Moringa. The pH decreased in all concentrations, unlike color, which increased in all tests. The longer the contact time of moringa with the raw water, the higher the turbidity. The concentration of microorganisms indicates that the raw water of Araçá lagoon is much higher than the maximum limit determined by the legislation. As for the combination of the use of Jar Test and the reduction in the concentration of moringa, it was observed that there was no change in the microbial load.