01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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2 resultados
Resultados da Pesquisa
Item Caracterização eletroquímica das interaçôes da lectina de sementes de Cratylia mollis (Cramoll) com glicose e eritrócitos de coelho em biossensor de MOF de [Cu3(BTC)2.(H2O)3]n(2022-05-31) Carvalho, Maryana Hermínio de; Coelho, Luana Cassandra Breitenbach Barroso; Freitas, Kátia Cristina Silva de; http://lattes.cnpq.br/5859266863241551; http://lattes.cnpq.br/2944428818449047; http://lattes.cnpq.br/0134945103585503Biosensors are small devices known for their selectivity, high specificity and sensitivity with the respective analyte even at low concentrations. Thus, we aimed to evaluate the charge transfer and electrochemical potential of the plant popularly known as camaratu bean, a forage native to the Caatinga biome, rich in shrub legumes, from which Cramoll lectin is extracted from Cratylia mollis Mart. electrochemical biosensor using the crystalline polymer MOF- [Cu3(BTC)2.(H2O)3]n.. to immobilize and characterize the physical and chemical properties of C. mollis (Cramoll) seed lectin and its interaction with free carbohydrates (glu-cose) and carbohydrates on the surface of rabbit erythrocytes. Lectin immobilization in MOF was effective for the construction of the electrochemical system, showing itself to be sensi-tive to conformational changes, being able to detect changes in the electrochemical poten-tials of Cramoll promoted in a buffered medium (200 mM potassium phosphate buffer, pH 7.0) with different glucose concentrations, highlighting the concentrations of 10 mM (96 - 121 mV), 15 mM (110 - 126 mV) and 20 mM (107 - 142 mV) in the time of 5 to 30 min. In the cyclic voltammetry, the interaction in the potential range from -0.2 to 0.0 V stood out; with the current reaching 0.0003 A and at peak 2 in the potential range of 0.4 to 0.5 V; with the current reaching 0.0007 A. The interaction of the carbohydrate present on the surface of the rabbit erythrocyte was also monitored by the potentiometric method, detecting changes in the Cramoll-glucose potentials, even in the presence of rabbit erythrocytes. Through SEM, it was possible to observe Cramoll immobilized on the MOF surface, proving the specificity of the ligand (glucose-lectin) through the morphological changes of this protein in this process. This electrochemical model, Cramoll/MOF biosensor, is effective for evalu-ating free lectin/carbohydrate or in the erythrocyte membrane.Item Proposta de uma sequência didática para o ensino de eletroquímica: biossensor como ferramenta de intervenção didática no processo de ensino-aprendizagem(2020-11-05) Silva, Suzi Batista da; Souza, Sandra Rodrigues de; http://lattes.cnpq.br/4678692939311710; http://lattes.cnpq.br/2869555050306545To mitigate the main difficulties encountered in the teaching and learning process, methodologies are proposed with a focus on didactics more focused on research, investigation and experimentation actions, which may provide more active strategies from the didactic and pedagogical point of view, facilitating the participation of the student. student in this process. The contents in electrochemistry have been frequently pointed out by high school teachers and students as one of the subjects that represents great difficulties in the teaching - learning process. In view of the difficulty exposed, this research aimed to develop a didactic sequence to alleviate the learning difficulties in teaching electrochemistry using the biosensors as input. The teaching methodologies proposed for the study of the concepts about galvanic cells (batteries) involve practical experimental activities through investigation and problem solving (problem situation). For this purpose, it was proposed to use the functioning of electrochemical biosensors as a didactic tool for conceptual knowledge applied to electrochemistry. In this way, the use of specific methodologies for teaching chemistry promotes significant results, allowing the student to reflect and resolve the contradictions of problematic situations as a resource in the construction of concepts, making the student's learning process effective.