01.1 - Graduação (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2
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Resultados da Pesquisa
Item Diversidade de espécies de Colletorichum associadas à antracnose do Inhame no estado de Pernambuco(2024) Silva, Myrella Graziela Gomes da; Câmara, Marcos Paz Saraiva; http://lattes.cnpq.br/3368616411048863; http://lattes.cnpq.br/4009798926901196Item Levantamento e análise da variabilidade genética do gênero Siderastrea Blainville, 1830(2024-10-04) Lira, Jean Tácio Tôrres de; Amaral, Fernanda Maria Duarte do; http://lattes.cnpq.br/7026011892824176; http://lattes.cnpq.br/0569107903225207This study aimed to fill gaps in knowledge about the genetic and phylogenetic diversity of the genus Siderastrea (Blainville, 1830), important coral reef-building organisms, with a greater focus on the species Siderastrea stellata (Verrill, 1868). Partially bleached colonies of the species S. stellata were collected on Gaibu beach, from November 2022 to January 2023 and the genomic DNA of the colonies was extracted using the Wizard Genomic DNA Purification Kit. A specific primer pair was designed to confirm the species S. stellata and the identification of the colonies was performed by the conventional PCR technique. The analysis of the variability between the colonies was performed by the RAPD technique using 06 random ITS primers. Furthermore, to analyze whether there was genetic variability among the S. stellata samples in the Genbank genomic database, we downloaded all sequences and performed sequence alignment. Subsequently, phylogenetic analyses were performed among the deposited samples using the Mega platform. Finally, in silico restriction enzyme analyses were performed to search for restriction enzymes that could be used to differentiate between the isolates. The results obtained with the RAPD technique did not provide sufficient data to identify genetic variability among the samples analyzed in the present study. On the other hand, regarding the data from the phylogenetic analysis, it was possible to find differences between the sequences of isolates from different regions. There was greater genetic proximity between S. stellata isolates from Panama and Brazil and less proximity to the isolate from Mexico. Siderastrea savigneana stood out as the most distant ancestor within the genus. In addition, it was possible to detect preliminary genetic variations among the populations evaluated, using data from the in silico restriction analysis. Thus, this study provides a first approach to genetic analysis of variability within the species of S. stellata. However, more in-depth studies are needed and may help to clarify the complexity of the genetic variability of the genus, in addition to assisting in management and conservation strategies for these organisms and the marine ecosystems they support.Item Estudo anatômico do cerebelo de Bradypus variegatus, Schinz 1825 (Mammalia: Pilosa)(2022-05-24) Oliveira, Ueliton da Silva; Amorim, Marleyne José Afonso Accioly Lins; http://lattes.cnpq.br/1237734889563996; http://lattes.cnpq.br/9250603868698523The common sloth (Bradypus variegatus, Schinz 1825) is an arboreal quadruped species belonging to the Class Mammalia, Superorder Xenarthra and Family Bradypodidae. In South America, it inhabits from northern Colombia to southwestern Venezuela, southern Ecuador, eastern Peru and Bolivia, in addition to being present in Brazil. In the case of organic systems, bradypodids have certain anatomical peculiarities such as double posterior vena cava, six to nine cervical vertebrae depending on the species; common duct for the urinary and genital tracts in females and internal testes in males. However, there are few studies concerning the central nervous system, especially when comparing them to domestic animals. Therefore, the present work aims to analyze, describe and morphologically characterize the cerebellar structures of the Bradypus variegatus sloth. 10 animals were used for the analyses, obtained after natural death from the Wild Animal Screening Center, and fixed in 20% formaldehyde. Reserved in numbered tanks in 30% saline solution, the animals also received individual identifications. The skullcap and brain were removed to gain access to the cerebellum. The organ was separated from the brain stem by a cross section and preserved in a 20% formaldehyde solution in glass reservoirs. The cerebellar structure of common sloths, in both males and females, was, on average, 1.21 cm long by 1.62 cm wide. From the analysis carried out, it is concluded that the B. variegatus cerebellum has an ovoid morphology, slightly flattened in the cranial and caudal lateral portions, presenting the worm in the central region, right and left hemispheres with lobes and fissures, which corroborate with descriptive studies performed on humans and domestic animals.Item Clima atual e passado como condutor da diversidade beta funcional e filogenética de aranhas ao longo do gradiente latitudinal(2018-02-26) Soares, Gabrielle Christina Firmino; Souza, Thiago Gonçalves; Oliveira, Gustavo Ribeiro de; http://lattes.cnpq.br/0444294877812617; http://lattes.cnpq.br/3342841776147612Climatic gradients can affect spatial and temporal distribution, as well as the morphological differentiation of organisms. The theory predicts that phylogenetically close species have larger body sizes in colder regions because of the need for heat storage; this explanation has been attributed to Bergmann's rule. However, the applicability of this rule to arthropods is still a challenge. Our objective was to test whether the spatial (over 2,000 km latitudinal gradient) and temporal (over 120,000 years) climate variability affect the phylogenetic and functional diversity of spiders. We used data from the literature (Gonçalves-Souza et al., 2014) that were collected in Restinga areas from southern Bahia to Santa Catarina in an extension of 12° 34'10 'at 27° 37'9' latitude. The functional attributes obtained were: height, length and width of the prossome and the length of the opistossoma. The phylogenetic tree was constructed from topologies at family and gender level. To test the effect of recent and past climate on functional and phylogenetic diversity, we used a Principal Coordinate Analysis (PCoA) combined with a Distance Based Redundancy Analysis (dbRDA) with the climate being the independent variable. Our results demonstrated that phylogenetically close spiders do not share similar morphological attributes (i.e., low phylogenetic signal). In addition, we found that stability favors the occurrence of smaller spiders. The maximum temperature of the hottest month of the past was the variable that most explained the variations of the functional and phylogenetic diversity of the spiders. The variables that indicate extreme temperature values and the annual precipitation of both the recent climate and the past were those that affected the body size most strongly. In this way, we can affirm that the seasonality and climatic extremes are dictating the variation of the body size and that this also reflects in the kinship relations of the spiders. Given the global climatic variations expected over the next 50 years, our results may help to understand possible future patterns of spatial distribution of arthropods.Item Avaliação do índice de parentesco entre proles de irmãos de Pacamã (Lophiosilurus alexandri Steindachner, 1876) Mantidos Nas Estações De Piscicultura Ao Longo Do Rio São Francisco(2019-12-13) Silva, Bruno Cezar Nascimento Ramos da; Coimbra, Maria Raquel Moura; http://lattes.cnpq.br/7669497233462075; http://lattes.cnpq.br/5741204185454039