01.1 - Graduação (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2
Navegar
8 resultados
Resultados da Pesquisa
Item Efeitos da elicitação isolada e combinada de ácido salicílico e metil jasmonato nos parâmetros biométricos e bioquímicos em Lippia alba (Verbenaceae)(2024-09-27) Paula, Maria Eduarda Magalhães de; Sperandio, Marcus Vinícius Loss; http://lattes.cnpq.br/4157291425794314; http://lattes.cnpq.br/8710784594902570Lippia alba is an aromatic shrub from the Verbenaceae family, primarily associated with Brazil's Caatinga and Cerrado regions. It is used in traditional medicine for its pharmacological properties, those being attributed to the essential oil the plant produces as a defense mechanism. A tool that can be used to increase the productivity of bioactive compounds in both in vitro and in vivo development is elicitation. This study aims to analyze the effects of isolated and combined applications of the elicitors Methyl Jasmonate and Salicylic Acid on L. alba, using biochemical and biometric data as parameters one week after the elicitation. Cuttings of L. alba were maintained in hydroponic cultivation and later transferred to a greenhouse. The combined and isolated treatments received a concentration of 4 mM Salicylic Acid and 2 mM Methyl Jasmonate. An additional group was established, receiving an application of 1% ethanol. The experiment was harvested one week after the elicitation. Elicited treatments showed significant differences in essential oil yield, oxidative stress analysis, and enzymatic activity compared to the control. Growth parameters, along with carbohydrate and protein levels, kept similar patterns in all treatments. In vivo, combined Salicylic Acid and Methyl Jasmonate elicitation increased essential oil yield on L. alba without affecting biomass production, suggesting an efficient modulation of the plant metabolism. Therefore, elicitation at the tested concentrations demonstrated to be a viable tool to optimize the production of bioactive compounds in this species in a short elicitation period.Item Influência da adição de NPK no crescimento, respostas bioquímicas e estresse oxidativo em Pavonia humifusa A. St.-Hil. (Malvaceae) sob estresse hídrico(2024-09-27) Nóbrega, Marina Morais; Sperandio, Marcus Vinícius Loss; http://lattes.cnpq.br/4157291425794314; http://lattes.cnpq.br/6953276255530257Water stress can trigger a series of plant metabolic changes, affecting their biochemistry and morphology. The variability of rainfall cycles creates challenges to adaptation. However, nitrogen supplementation (NPK) can play a crucial role in this context, as these nutrients are fundamental for maintaining metabolism, especially under stress conditions. The present study evaluated the effects of nutritional supplementation on the biochemical and morphological responses of Pavonia humifusa plants under water stress. After 21 days of supplementation, water stress was induced for 29 days. The experiment included two NPK treatments: with and without NPK associated with two conditions of water availability: control and stress. Biometric parameters (aerial and root dry biomass), physiological parameters (relative water content and SPAD index), primary metabolism parameters (pigment and proline content), stress markers (malondialdehyde and hydrogen peroxide) and enzyme activities (superoxide dismutase, catalase and ascorbate peroxidase) were analyzed. ANOVA analyzed the data at 5% and the means were compared by the SNK test using the R software. The analyses carried out indicate that after nutritional application with NPK, P. humifusa showed a better growth rate, as well as a higher total chlorophyll content in vivo. The plants supplemented with NPK also showed better adaptation to water stress with a reduction in oxidative stress. In addition, with NPK supplementation, P. humifusa responded to be less susceptible to water stress by increasing the enzymatic activity of SOD and Proline, the main osmoregulatory amino acid in water stress conditions. The results obtained suggest that nutritional supplementation with NPK plays an important role in biochemical and physiological regulation against water stress in P. humifusa.Item Fertilização potássica no crescimento de mudas de Eucalyptus camaldulensis Dehnh. sob limitação hídrica(2024-03-04) Luna, Andressa Cristina de; Freitas, Eliane Cristina Sampaio de; http://lattes.cnpq.br/7525975084334972; http://lattes.cnpq.br/8800788371861007The objective of this study was to evaluate the effect of potassium fertilization on the growth of Eucalyptus camaldulensis Dehnh seedlings under water limitation. The sowing was done with improved seeds of E. camaldulensis Dehnh in 80 tubes of 55 cm³ each. The seedlings were grown in full sunlight, and topdressing fertilization with macro and micronutrients, along with different doses of potassium (K), using potassium chloride (KCl) as a source, was applied. After the potassium fertilization was completed, measurements of height (H) and collar diameter (CD) were taken to standardize the seedlings according to each treatment (K dose), and they were transplanted into 5 dm³ capacity pots filled with a substrate composed of 70 % subsurface soil and 30 % commercial substrate. The treatments consisted of a factorial of two irrigation regimes (with and without suspension) by five levels of topdressing fertilization with K (0, 75, 150, 225, and 300 mg dm - ³), arranged in a completely randomized design with 4 repetitions, each pot composing a sample unit. After 15 days of transplantation, irrigation was suspended in half of the pots for 21 days, and the number of leaves (NL) was counted, along with recording visual symptoms of water stress during this period. At the end of the experiment, new measurements of height (H), collar diameter (CD), shoot dry matter (NL) were taken, growth increments were calculated, and stomatal density, chlorophyll index, relative water content, and dry matter were determined. Using the data of height (H), collar diameter (CD), shoot dry matter (SDM), root (RDM), and total (TDM), seedling quality ratios were calculated: SDM/RDM, H/CD, and Dickson Quality Index (DQI). The data were analyzed using analysis of variance (p<0.05), and those indicating significant interaction were subjected to regression analysis within the qualitative factor. Analyzing the statistical data, it was observed that potassium fertilization positively influenced the initial growth of the seedlings in all treatments, with the estimated optimum dose being 286 Kg dm -3 of K. The result of the variables with the factors of K doses and irrigation of Eucalyptus camaldulensis Dehnh seedlings showed a significant difference of 5% only for irrigation.Item Memória hídrica (stress imprint) em sementes: impacto ecofisiológico na germinação e no crescimento inicial de Sesbania virgata (Cav.) Poir(2024-03-11) Menezes, Mariane Oliveira; Santos, Marcone Moreira; Silva, Carlos Luiz da; http://lattes.cnpq.br/9191002336120487; http://lattes.cnpq.br/3284707164193715; http://lattes.cnpq.br/1484285198376822The present study investigated the effects of hydraulic memory on the eco-physiological aspects of germination and the initial development of S. virgata seedlings. Employing a mixed-method approach that combined quantitative and qualitative methods, along with rigorous statistical analysis including tests such as Tukey, ANOVA, and regression analysis, a randomized experiment with various treatments was conducted. Initially, a literature review was conducted to understand the species' importance and the effects of hydraulic memory. Subsequently, laboratory tests were performed to overcome seed dormancy, determine moisture content, and establish the imbibition curve. Additionally, the germination rate of seeds subjected to polyethylene glycol 6000 (PEG 6000) solution was evaluated to simulate water stress, both with seeds that underwent intermittent hydration cycle (IHC) and those that did not undergo this process. Following laboratory analyses, nursery analyses were conducted with two distinct treatments (seeds subjected to IHC and seeds that did not undergo this process), under different watering regimes. Evaluated parameters included shoot height and diameter, root size, dry and fresh weight, Dickson's quality index, biomass allocation, and seedling survival rate. The results indicated the possible presence of hydraulic memory in the seedlings and demonstrated that water deficit negatively affects germination. However, intermittent hydration showed a positive effect on seed germination under water deficit conditions, offering a promising strategy to enhance plant survival in adverse environments.Item Restrição hídrica e fertilização fosfatada no crescimento de mudas de Tabebuia aurea (Silva Manso) Benth. & Hook.f ex S. Moore sob diferentes regimes de irrigação(2023-09-20) Pereira Neto, Cláudio Clementino; Freitas, Eliane Cristina Sampaio de; http://lattes.cnpq.br/7525975084334972; http://lattes.cnpq.br/5841590477669007The availability of water in the soil is one of the most important factors influencing the successful establishment of saplings in field conditions. The scarcity of this resource serves as a constraining element that hampers both productivity and the prosperous cultivation of forest crops. Implementing applications of phosphate fertilizer can play a pivotal role in fostering the development of a more robust root system, thereby potentially mitigating the adverse impacts stemming from inadequate water availability on the cultivation. Investigations dedicated to silviculture pertaining to native forest species remain relatively scarce, primarily due to a concentration of research efforts around commercially more prevalent species. With the aim of evaluating tolerance and growth, as well as morphophysiological alterations in seedlings of Tabebuia aurea (Silva Manso) Benth. & Hook.f ex S. Moore subjected to water deficit and phosphate fertilization; an experiment was conducted at the greenhouse of the Department of Forestry Science at UFRPE – SEDE. Three irrigation cycles: daily (T); every 5 days (S5); and every 10 days (S10), and 4 phosphorus addition treatments (0, 100, 200, and 300 mg dm-³ of P) were tested following a factorial scheme in a completely randomized design, with 5 replicates. The source of P employed was monosodium phosphate (NaH2PO4). Two seeds were placed per 50 cm³ tubes, and thinning was conducted post germination. Following germination and growth, seedlings were standardized based on height and transplanted into 2,8 dm³ pots containing subsoil substrate. Water deficit induction was conducet after transplantation and phosphorus treatments differenciation. Throughout the experiment, seedling measurements were taken every ten days, encompassing parameters such as height (H) and stem diameter (DC). At the conclusion of the trial, the following aspects were determined: shoot dry matter (MSA), root dry matter (MSR), total dry matter (MST), shoot-to-root dry matter ratio (MSA/MSR), and Dickson's Quality Index (IQD) were calculated. Additionally, H, DC, NF, leaf area, primary root length, and chlorophyll content were determined. The application of phosphorus doses did not act as a mitigating factor for the effects of water stress. Seedlings with better quality were obtained in the treatment with daily irrigation, and the dose that yielded the best responses for the production of T. aurea seedlings was 200 mg dm-3.Item Influência da sazonalidade sob o acúmulo de osmorreguladores em Pavonia varians Moric, espécie endêmica da Caatinga(2022-10-06) Barros, Yasmim Lopes de; Nunes, Larisse Bianca Soares Pereira; Silva, Cláudia Ulisses de Carvalho; http://lattes.cnpq.br/7161911278790052; http://lattes.cnpq.br/5742230717504216; http://lattes.cnpq.br/8411583457051354The Caatinga is a Seasonally Dry Tropical Forest in Brazil, considered one of the most diverse semi-arid regions in the world, with a high number of endemic species and a high living population that is dependent on forest resources. Despite its importance, this region has been suffering major environmental impacts caused by human actions due to the exacerbated use of its resources. This makes it a vulnerable forest to the effects of climate change, resulting in even longer periods of drought and an increase in temperature that can result in reduced biodiversity and species abundance. In this scenario, plants make use of several mechanisms to avoid desiccation and one of them concerns the accumulation of osmoregulators. Through this knowledge, this work aims to evaluate the performance of osmoregulators in Pavonia varians, an endemic species of the Caatinga, during the dry and rainy season. For this, leaves of P. varians were collected in the field to determine the concentrations of photosynthetic pigments, carbohydrates, sucrose, proteins and proline in relation to the rainy and dry seasons. The data obtained were analyzed via ANOVA and the means compared by the Tukey test 5%. Our results show that the quantification of pigment, carbohydrate, protein and proline contents in the leaves did not vary between the dry and rainy seasons. In addition, we evidenced an accumulation of sucrose in the leaves during the dry period, suggesting its importance as an osmotic adjuster so that the species can remain with leaves during the dry period. Finally, the preference of P. varians for the greater accumulation of sucrose in the leaves as a function of the dry period can be considered an innate behavior of the species.Item Efeitos do peróxido de hidrogênio (H2O2) na morfologia e bioquímica de plantas de Lippia grata Schauer submetidas a restrições hídricas(2022-10-06) Silva Filho, Josias Alexandre da; Silva, Cláudia Ulisses de Carvalho; http://lattes.cnpq.br/7161911278790052; http://lattes.cnpq.br/8117701230694209Lippia grata belongs to the Verbenaceae family, is native to Brazil found in the phytogeographic domains of the Cerrado and Caatinga, Atlantic Forest and Amazon. It is popularly known as alecrim-da-chapada and alecrim-do-mato, which is widely used as a medicinal product, due to the essential oil present in its leaf trichomes, which have bioactives with antiseptic, antimicrobial, antifungal and antibacterial properties. Because it is located in semi-arid environments, water becomes the main limiting factor for the species, and therefore strategies to mitigate the harmful effects of water deficit have been used, such as the application of signaling molecules (elictors), such as hydrogen peroxide, in order to assist plants in conditions of intensified drought stresses. Taking into account the importance of L. grata and its essential oils, since water deficit can affect its development, the objective of the following study was to evaluate the influence of hydrogen peroxide on the biometric and biochemical parameters in L grata subjected to restriction water For this, the leaves of L. grata plants were sprayed with H2O2 at concentrations of 0μM (control), 0.675μM L-1 and 1.35μM L-1 and then subjected to three irrigation regimes: 25%, 50% and 75% of vessel capacity for 60 days. Where biometric parameters and some osmoregulators were measured. The water restriction allied to the higher concentration of hydrogen peroxide (1.35μM L-1) negatively affected the growth and development of L. grata plants, being expressed in the biometric parameters (impairment of growth and production of branches), dry mass of shoots and increase in proline levels.Item Influência dos ciclos de rega na anatomia e metabolismo bioquímico foliar de Cenostigma pyramidale (Fabaceae)(2022-10-04) Alves, Jakelline Santos; Silva, Flávia Carolina Lins da; http://lattes.cnpq.br/7547725170997480; http://lattes.cnpq.br/8593632087425943The species Cenostigma pyramidale is especially important in the ecological aspect and little explored in the economic and environmental sphere, in view of its great potential, besides being used for timber and medicinal purposes is a pioneer species with high potential for the recovery of degraded areas. This work aims to evaluate the physiological and anatomical responses of the leaves of one of these species, Cenostigma pyramidale under irrigation cycles and submitted to water deficit. The plants were submitted to four experimental groups, during the growing period, counting ten individuals per treatment: Control - daily watering; RD - daily watering; R7 - watering 1x in 7 days; R14 - watering 1x in 14 days. After five months of growth in the greenhouse, the individuals were submitted to a period of water deficit, where there was complete suspension of water, and then underwent recovery, from which five individuals from each treatment were collected at the end of each period for biochemical analysis and leaf anatomy. To determine the periods, analysis of relative leaf water content (RWC) and soil moisture was performed. It was observed that plants from more severe treatments proved to be more resistant, in general, to maximum stress, anatomically and biochemically, investing in adaptations such as tannins, crystals and stomatal chambers, still showing expressive photosynthetic rates, indicating that pre-contact with short intervals of water shortage affects leaf storage responses during a prolonged water shortage. The most efficient use of resources was seen in the treatment that was subjected to irrigation every fourteen days (R14), which showed a high rate of total free amino acids, proteins, higher rate of photosynthetic pigments, presence of an abundant palisade parenchyma, while showing a tendency to decrease the volume of the mesophyll, among other adaptations. Thus, with this work it was possible to conclude that previous contact with lack of water positively affects the species Cenostigma pyramidale, generating more efficient responses and providing important anatomical and biochemical changes in its leaves.