TCC - Medicina Veterinária (Sede)
URI permanente para esta coleçãohttps://arandu.ufrpe.br/handle/123456789/475
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Resultados da Pesquisa
Item Avaliação do fluxo sanguíneo do folículo ovulatório e corpo lúteo de fêmeas bubalinas por ultrassonografia Doppler(2024-09-27) Farias Júnior, Francisco Timbó; Batista, André Mariano; http://lattes.cnpq.br/5615914349535394; http://lattes.cnpq.br/7562129132084802The Mandatory Supervised Internship (ESO) is an essential part of the training of a Veterinary Doctor, being a mandatory requirement in the Veterinary Medicine course at UFRPE. With a workload of 420 hours, the internship was conducted at the Laboratory of Biotechniques Applied to Reproduction (LBR), between 04/01/2024 and 06/14/2024. The focus was on improving reproductive biotechnology, with emphasis on the in vitro maturation of oocytes and the development of an article based on research carried out with Murrah buffaloes. The study evaluated the blood flow of the ovulatory follicle and corpus luteum using Doppler ultrasound, correlating these data with reproductive efficiency. In the laboratory, activities such as in vitro maturation of bovine oocytes (IVM) and evaluation of meiotic progression using epifluorescence microscopy were carried out. Under the guidance of Professor Dr. André Mariano Batista and supervision of Professor Dr. Cláudio Coutinho Bartolomeu, the internship provided a practical insights into reproductive biotechnologies and their impact on reproductive efficiency in buffaloes.Item Relatório de Estágio Supervisionado Obrigatório (ESO) realizado na Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Gado de Leite, Juiz de Fora e no Laboratório de Biotécnicas Aplicadas à Reprodução do Departamento de Medicina Veterinária da Universidade Federal Rural de Pernambuco: efeito da adição de nanoemulsão à base de óleo essencial rico em limoneno ao diluente de refrigeração de sêmen caprino(2024-02-15) Nottar, Luiz Henrique Todescatt; Batista, André Mariano; http://lattes.cnpq.br/5615914349535394; http://lattes.cnpq.br/3747311074315408The present study aims to report the activities carried out in compliance with the discipline 08525 - Mandatory Supervised Internship (MSI), part of the Bachelor's Degree in Veterinary Medicine, conducted between October 2, 2023, and November 28, 2023, at the Brazilian Agricultural Research Corporation (Embrapa), in the Dairy Cattle unit in the municipality of Juiz de Fora - MG, and between January 2 and January 19, 2024, at the Laboratory of Biotechniques Applied to Reproduction (LBR) of the Department of Veterinary Medicine (DMV) of the Federal Rural University of Pernambuco (UFRPE). The activities carried out provided in-depth training in in vitro embryo production (IVP) techniques for research purposes, including follicular aspiration of ovaries from abattoirs, in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro embryo culture (IVC). Activities in the field of molecular biology involving DNA extraction and amplification through the Polymerase Chain Reaction (PCR) technique were also performed. In the second chapter of this study, a research was conducted to evaluate the effect of adding nanoemulsion based on limonene-rich essential oil extracted from wild orange peel (NELS) to the semen extender of goat breeders. Seminal samples from five Saanen breed goats were used for the research. Each seminal pool was diluted in Tris-egg yolk supplemented with NELS at concentrations of 1.25, 2.5, and 5% at a concentration of 200 × 106 sperm/mL. The samples were placed in Eppendorf tubes (1.5 mL) and stored in refrigeration boxes (botuflex®) for cooling curve determination up to 5 ºC, and after 2 hours, they were transferred to the refrigerator, also at a controlled temperature of 5 ºC. For each analysis, part of each sample was aliquoted and transferred to other Eppendorf tubes (1.5 mL) in a dry bath at 37 ºC and subsequently evaluated in the Computer-Assisted Sperm Analysis System (CASA) for sperm kinetics, and in epifluorescence microscopy for Mitochondrial Membrane Potential (MMP), Plasma Membrane Integrity (PMI), and Acrosomal Membrane Integrity (AMI). The addition of wild orange nanoemulsion to the Tris-egg yolk extender was not able to improve sperm motility and kinetic parameters, besides significantly reducing MMP after 24 hours of refrigeration.