01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Caracterização molecular de determinantes de resistência a antibióticos de Salmonella spp. isoladas de carne de frango in natura comercializados em mercados públicos de Recife - PE(2020-02-03) Silva, Marcela Oliveira da; Souza, Paula Mariana Salgueiro de; http://lattes.cnpq.br/6281410502740086; http://lattes.cnpq.br/9549169192404311Salmonella is one of the main causative agents of foodborne infections. Salmonellosis becomes more dangerous when strains resistant to various antibiotics are found in food, especially in chickens, one of the main vehicles for transmitting this pathogen to humans. The aim of this study was to characterize the determinants of antibiotic resistance present in Salmonella spp. isolated from fresh chicken meat sold in public markets in the city of Recife. For that, 21 isolates of Salmonella spp. underwent phenotypic and molecular tests to assess susceptibility to antibiotics and to investigate determinants of resistance to [beta]-lactams and quinolones. The results obtained demonstrated the presence of three large clonal groups among bacterial isolates, in addition to the presence of genes encoding [beta]-lactamases and genes that confer resistance to quinolones. The spread of different polyclonal groups of Salmonella spp. MDR, in chicken carcasses, shown in this study, reinforces the need for effective controls to contain this microorganism, which in addition to being a risk to public health, is also responsible for considerable economic losses.Item Isolamento e detecção de bactérias produtoras de B-lactamases de espectro estendido (ESBL) oriundos de infecção do trato urinário em cães e gatos atendidos no hospital veterinário da Universidade Federal Rural de Pernambuco(2022-02-24) Oliveira, Leonardo Bruno Sampaio de; Cavalcanti, Erika Fernanda Torres Samico Fernandes; http://lattes.cnpq.br/5256493441853885; http://lattes.cnpq.br/4755887972258322Item Avaliação in vitro de derivados 1,2-alcanodiamina frente ao Mycobacterium tuberculosis sensível e multidroga resistente(2019-07-09) Laet, João Paulo de Lucena; Pimentel, Lílian Maria Lapa Montenegro; Burgos, Fábia Regina Nascimento Fernando; http://lattes.cnpq.br/3776642205741967; http://lattes.cnpq.br/6066865382706623; http://lattes.cnpq.br/3972432719752724Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB), an infectious-contagious disease, considered one of the deadliest in the world. TB remains a major global public health problem and so far, there has been no effective way to eradicate it. A major change in TB trajectory occurred with the introduction of the first anti-TB drugs and consequently the emergence of resistance forms to antimycobacteria, resistant multidrug tuberculosis (MDR-TB) and extensively resistant tuberculosis (XDR-TB). From this, the need arises for new drugs to be proposed in the treatment of this disease. The present study aims to evaluate diamine derivatives, related to ethambutol, against sensitive Mtb and MDR. Ten compounds derived from the class 1,2-alkanediamine were synthesized and characterized structurally by the pharmaceutical chemistry laboratories of the University of Salamanca and provided through international cooperation. For the biological tests, the standard reference strain H37Rv (ATCC 27294) was classified as a strain sensitive to all drugs used to treat TB and a clinical isolate of a patient affected by pulmonary tuberculosis with a MDR profile, provided by the Health Laboratory Public of Pernambuco. Minimum Inhibitory Concentration (MIC) determined the evaluation of compound activity in 96-well plates by the colorimetric microdilution method. For cytotoxicity (CC50) the MTT test (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) was used with the J774A.1 cell line. The selectivity index (IS) was calculated by the ratio of MIC and CC50. The ten compounds tested had a MIC ranging from 4.0 to> 64.0 μM for strain H37Rv and MDR. With respect to CC50, the compounds had a concentration range ranging from 3.9 to 32.8 μM. Compound D15 exerted the best activity against the reference strain (H37Rv) and MDR strain (MDR 1576), however in the cytotoxicity test the compound that presented the best result was D10. Regarding the selectivity index, compounds A4 and A5 were the ones that obtained the best results even though they were below the ideal index. The compounds evaluated in this study showed moderate activity against Mtb and support the continuity of the study of 1,2-alkanediamine derivatives.