01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Glicerina bruta produzida pela Biorrefinaria Berso: purificação e preparação do carbonato de glicerol(2022-10-17) Ribeiro, Rubson Patrik Sena; Araújo, Patrícia Lopes Barros de; Oliveira, Ronaldo Nascimento de; http://lattes.cnpq.br/9071551767043294; http://lattes.cnpq.br/9826955590432737; http://lattes.cnpq.br/7729510453800791Since the last century, the perception of the rampant use of fossil fuels based on oil and natural gas is inevitable. Allied to this, we see a race in search of energy sources that exceed the socioeconomic demand and production of the current world. In Brazil, there is a fertile soil for generating raw material for biodiesel production, due to the large amount of vegetable and oilseed species such as castor bean, palm, sunflower, babassu, peanut, jatropha, soybean, in addition to animal raw material, such as beef tallow, among others. From the transesterification reaction of the triglycerides of an oil or fat with an alcohol, methyl (or ethyl) esters of fatty acids are produced, which are biodiesel. Glycerol is a co-product of this reaction, which receives the commercial designation of glycerin, for samples containing more than 95% of the glycerol compound. The increase in the global supply of glycerin has led to the search for alternatives for the consumption of this co-product in its raw form and/or as derivatives with high added value. This work is based on the inclusion of glycerin as an abundant and cheap input in the high value-added polymer industry. The strategy was based on obtaining glycerol carbonates, a monomer for the synthesis of biodegradable glycerol polycarbonates, from purified glycerin. The results were obtained by obtaining a purified glycerol with 82% purity and a glycerol carbonate with more than 85% purity, both results from the analysis of chemical interactions and characterizations of the products obtained using Fourier Transform Infrared (FTIR), hydrogen and carbon-13 nuclear magnetic resonance (1H and 13C NMR) and Thermogravimetric Analysis (TGA). In this way, this work gains the possibility of solving practical problems related to obtaining advanced polymeric materials for use in industries and laboratories.Item Recuperação e purificação parcial de proteases colagenolíticas de tainha (Mugil liza) usando precipitação e particionamento em sistemas de fases(2020-02-03) Costa, Beatriz de Aquino Marques da; Porto, Ana Lúcia Figueiredo; http://lattes.cnpq.br/4989617783837981; http://lattes.cnpq.br/2650705679652460Proteases play an important role in the field of biotechnology studies, which is why the research for alternative sources is highly desirable. Thus, combining extraction, recovery and purification techniques to alternative sources, such as by-products of the fishery production chain, in order to generate the least possible damage to the aquatic environment is profitable for the global enzyme market. Therefore, the objective of the present work is to partially recover and purify collagenolytic proteases from Mullet (Mugil liza) digestive viscera with potential biotechnological application. For this, the digestive viscera extract (intestine, liverand a mixture of various viscera) was precipitated using ammonium sulfate ((NH4) 2SO4). In addition, the intestinal viscera were submitted to partitioning by an Aqueous Two-Phase System (ATPS) and a Three-Phase Partitioning system (TPP), to evaluate the most beneficial conditions for the purification of collagenolytic proteases. The results obtained demonstrate that, for ammonium sulphate precipitation, the best results of Purification Factor occurred in concentrations of 30-60% for all assessed extracts (intestine, liver and mix); the aqueous two-phase system (PEG/Citrate) carried out with Mullet intestinal viscera extract demonstrates that the conditions: PEG of 8000 g/mol molar mass; 20% concentration of PEG; 15% citrate concentration lead to the highest purification factor. It is also observed that the collagenases tend to migrate to the PEG-rich phase; for the three-phase system (t-butanol / (NH4) 2SO4) the highest recovery rate of collagenolytic protease was observed in the proportion of 1:0.5, obtaining recovery of 240.01% and a purification factorof 2,55. Thus, it is concluded that the tests can be used in the recovery of biomolecules from neglected organic solid waste from the fishing industry.