01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Investigação do potencial antibiofilme da lectina de folhas de Schinus terebinthifolia Raddi contra Staphylococcus aureus(2019-12-20) Silva, Talyta Naldeska da; Pontual, Emmanuel Viana; Silva, Pollyanna Michelle da; http://lattes.cnpq.br/0563176148137978; http://lattes.cnpq.br/1777060469196142; http://lattes.cnpq.br/8762925956752737Biofilms are complex microbial communities that have been associated with the incidence of infections, including in the hospital environment. Its formation is considered a determining factor for virulence in microorganisms and strongly contributes with the microbial resistance to conventional antimicrobials. In this sense, the search for natural compounds with antimicrobial activity that are more effective and less toxic to host cells. Plants are important sources of bioactive compounds, including lectins, a class of carbohydrate-binding proteins whose antimicrobial action has been reported. SteLL is a leaf lectin isolated from Schinus terebinthifolia Raddi (Anacardiaceae) with antimicrobial action that was previously reported. In this work, the antibacterial and antibiofilm potential of SteLL against sensitive (UFPEDA 02) and oxacillin resistant (UFPEDA 670) isolates of Staphylococcus aureus was evaluated. SteLL was a bacteriostatic and bactericidal agent against UFPEDA 02 and UFPEDA 670 isolates with MIC50 (Minimum Inhibitory Concentration) of 12.5 and 25.0 μg/mL and CMB (Minimum Bactericidal Concentration) of 50.0 and 100 μg/mL, respectively. The growth kinetics of the cells treated with SteLL revealed a dose-dependent growth inhibition regarding to control. SteLL caused morphometric alterations in S. aureus cells and inhibited biofilm formation of UFPEDA 02 at concentrations between 400 and 25.0 μg/mL and UFPEDA 670 between 400 and 100 μg/mL. In conclusion, SteLL is an antibacterial agent against Staphylococcus aureus by inhibiting the growth, promoting cell death and inhibiting bacterial biofilm formation.Item Análise in silicode iniciadores de genes referência utilizados como normalizadores em estudos utilizando qPCR para avaliação da expressão de isolados de Klebsiella pneumoniae(2019) Fonseca, Bárbara Schneyder Oliveira Pereira da; Almeida, Anna Carolina Soares; Nascimento, Crisvânia Pedrosa dos Santos; http://lattes.cnpq.br/9308656350291661; http://lattes.cnpq.br/4891800920829895; http://lattes.cnpq.br/0924501124844316Klebsiella pneumoniaeis a pathogenic bacterium considered to be an "urgent threat to human health"becausethe number of antibiotic-resistant bactéria is increasing, especially those considered to be the last line for its treatment, such as colistin.Forthis it is necessary to understand their mechanisms of resistance to know the best forms of treatment and to develop new drugs to treat infections. For this purpose, real-time quantitative PCR in relative expression studies has become one of the most effective tools to understand bacterial functioning at the transcriptional level, but for the results to be reliable and real, it is necessary to perform the normalization step, which among the possible the most common is through the useof reference genes. However, the choice of the genes to be used as normalizers among the genes pointed out in the literature has been controversial and, in many cases, with little reliability. This difficulty would be eliminated if there were a robust database for various types of studies for species other than humans and rats. Thus, there was a need to evaluate amongthe bacterial gene expression studies using qPCR, as normalizing genes and the primers used to amplify them.In a literature review, available in Pubmed, the 16Sand rpoBgenes were most commonly used as normalizers. Through in silico analysis after literature analysis it was possible to observe that the sequences of shared primer pairs were only smaller series within the ideal standard and the best ones in the future usedin later experiments, among others only one referring to one of the genes most commonly used, the 16S.