01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Seleção genética em mudas de Schinus terebinthifolia Raddi submetidas a condições ambientais(2023-08-30) Silva, Marcos Antônio Dias da; Gallo, Ricardo; http://lattes.cnpq.br/5160912065817980; http://lattes.cnpq.br/6163445855357563The red pepper tree (Schinus terebinthifolia Raddi) belongs to the Anacardiaceae family, has great economic relevance and its fruits are known as pink pepper. Intense exploration implies individuals that do not present fruiting or establishment problems, avoiding offspring that are not very vigorous and have low adaptability. This study aims to select Schinus terebinthifolia genotypes with good ability to develop seedlings under environmental conditions. The material was collected in 10 matrices located in Tejucupapo - Goiana/PE. The fruits were sent to the Department of Forestry Science (DCFL) at UFRPE, being processed and subjected to vigor testing. At the Forest Nursery of the same institution, the progeny test was carried out, with 10 seeds being sown per plastic bag, a substrate consisting of vermiculite, commercial substrate and subsoil soil (1:1:6). The environments were full sun and 50% shading (sombrite), with a 10x20x2 factorial scheme. Initially, %E was evaluated; IVE and the TME. And at 180 days: H; A.D; CR; %S; VIGOR; NF; IE; MSR and MSPA. The design was in randomized blocks, with one plant per plot, with 20 blocks, in each environment. The analyzes were performed using the SELEGEN-REML/BLUP software. The deviance test and the Mulamba and Mock selection index were carried out. The electrical conductivity test showed matrices M9, M2, M7 and M10 with the most viable seeds. The variation components of the progeny test in different environments did not present significant values, showing that the environment does not influence the germination and establishment of seedlings. Deviance showed that the characteristics IVE, %E and SOB were significant. These characteristics were used to create the average rank. There was a significant difference in the physiological quality of seeds. As there was no difference in the progeny test in the environments and due to the genetic parameters of the progenies, the average rank was used, classifying M9 and M2 with the best genetic materials.Item Investigação do potencial antibiofilme da lectina de folhas de Schinus terebinthifolia Raddi contra Staphylococcus aureus(2019-12-20) Silva, Talyta Naldeska da; Pontual, Emmanuel Viana; Silva, Pollyanna Michelle da; http://lattes.cnpq.br/0563176148137978; http://lattes.cnpq.br/1777060469196142; http://lattes.cnpq.br/8762925956752737Biofilms are complex microbial communities that have been associated with the incidence of infections, including in the hospital environment. Its formation is considered a determining factor for virulence in microorganisms and strongly contributes with the microbial resistance to conventional antimicrobials. In this sense, the search for natural compounds with antimicrobial activity that are more effective and less toxic to host cells. Plants are important sources of bioactive compounds, including lectins, a class of carbohydrate-binding proteins whose antimicrobial action has been reported. SteLL is a leaf lectin isolated from Schinus terebinthifolia Raddi (Anacardiaceae) with antimicrobial action that was previously reported. In this work, the antibacterial and antibiofilm potential of SteLL against sensitive (UFPEDA 02) and oxacillin resistant (UFPEDA 670) isolates of Staphylococcus aureus was evaluated. SteLL was a bacteriostatic and bactericidal agent against UFPEDA 02 and UFPEDA 670 isolates with MIC50 (Minimum Inhibitory Concentration) of 12.5 and 25.0 μg/mL and CMB (Minimum Bactericidal Concentration) of 50.0 and 100 μg/mL, respectively. The growth kinetics of the cells treated with SteLL revealed a dose-dependent growth inhibition regarding to control. SteLL caused morphometric alterations in S. aureus cells and inhibited biofilm formation of UFPEDA 02 at concentrations between 400 and 25.0 μg/mL and UFPEDA 670 between 400 and 100 μg/mL. In conclusion, SteLL is an antibacterial agent against Staphylococcus aureus by inhibiting the growth, promoting cell death and inhibiting bacterial biofilm formation.Item Efeito da lectina de folhas de Schinus terebinthifolia sobre a formação de vasos do saco vitelínico e morfologia de embriões de Coturnix japônica(2019-08-13) França, Rayssa Perla Martins; Pontual, Emmanuel Viana; http://lattes.cnpq.br/1777060469196142; http://lattes.cnpq.br/4999755464041290Angiogenesis corresponds to the budding of new blood vessels starting from other preexisting ones. This process is of great importance for development of tumor cells. Schinus terebinthifolia (Brazilian Peppertree) leaves express a thermostable and antimicrobial lectin (SteLL). This work reports the effect of SteLL on the angiogenesis process using the in vivo model of yolk sac membrane of Coturnix japonica (Japanese quail) embryos. The fractal analysis by box counting and information dimension indicated that SteLL at 1.35 mg/mL reduced the angiogenesis of C. japonica yolk sac membrane. The inhibition of the vascular network formation in the yolk sac membrane resulted in decreased blood supply to the embryos, and consequently area, perimeter and percentage of cephalic length of embryos were significantly reduced in the group treated with SteLL, corroborating with the antiangiogenic activity. In conclusion, SteLL is an antiangiogenic agent and this result stimulate future investigations about its antitumor activity.