01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
Navegar
9 resultados
Resultados da Pesquisa
Item Relatório de Estágio Supervisionado Obrigatório (ESO) realizado na Universidade Estadual Paulista Júlio Mesquita Filho município de Botucatu - SP, Brasil e na Universidade Federal Rural de Pernambuco, município de Recife - PE, Brasil: panorama dos crimes contra cães e gatos e investigação de leishmaniose e escabiose em animais sob tutela de protetores no município de Ipojuca - PE(2024-08-26) Andre, Cassia de Freitas; Oliveira, Andrea Alice da Fonseca; http://lattes.cnpq.br/2519790342576901; http://lattes.cnpq.br/4448146168412898The Mandatory Supervised Internship was conducted under the guidance of Professor Dr. Andrea Alice da Fonseca Oliveira. The activities took place in the pathology department of the Faculty of Veterinary Medicine and Animal Science at São Paulo State University (UNESP), Campus Botucatu - SP, from April 1st to April 30th, 2024, under the supervision of Professor Dr. Noeme de Sousa. Additionally, the internship included work at the Laboratory of Parasitic Diseases within the Department of Veterinary Medicine at the Federal Rural University of Pernambuco, Campus Recife - PE, from May 6th to June 19th, 2024, under the supervision of Professor Dr. Leucio Câmara Alves. In the pathology department, cytological examinations, anatomoclinical necropsies, and forensic necropsies were performed. At the Laboratory of Parasitic Diseases, services were provided in the canine leishmaniasis outpatient clinic, including routine tests such as the Knott, Flotac, and Mini Flotac techniques. During the internship period, an article titled ‘Overview of Crimes Against Dogs and Cats and Investigation of Leishmaniasis and Scabies in Animals Under the Care of Protectors in the Municipality of Ipojuca - PE was prepared. Data were collected from the 42nd and 43rd Police Stations in Ipojuca, and visits were made to animal protectors, with evaluation forms completed for dogs, cats, and scabies cases. Six cases from the 42nd police station and six from the 43rd were analyzed. Among the animals evaluated from the protectors, one dog tested positive for Leishmania infantum chagasi amastigotes, another dog had Demodex sp., and a cat tested positive for Notoedres cati. Additionally, in eight cats undergoing trichogram analysis, Lynxacarus radovskyi was found in 100% of cases. Regarding the animals’ history, 54.28% were rescued from abusive conditions. Thus, considering that the Leishmaniasis-positive dog was rescued from abandonment, we can infer, albeit tentatively, the significant connection between animal cruelty and its potential impact on public health.Item Detecção de doença cardiovascular ou diabetes utilizando machine learning(2024-03-07) Santos, Daniel Ramos Correia dos; Albuquerque Júnior, Gabriel Alves de; http://lattes.cnpq.br/1399502815770584Cardiovascular diseases and diabetes represent significant challenges for public health, requiring effective diagnostic and prevention approaches. This work proposes an approach based on machine learning models to support these processes. Using a database from the IBGE national health survey, the study investigated how different variables affect the detection of these diseases. Using algorithms such as Random Forest, XGBoost and SVM, predictive models were developed. The results demonstrated an accuracy of 71.96% for the Random Forest algorithm in classifying patients with cardiovascular diseases and 72.26% in classifying patients with diabetes. Analysis of the most influential variables was also carried out using the SHAP method, which revealed some insights into the data.Item Componentes orgânicos voláteis (COV) como biomarcadores da COVID-19(2023-04-28) Santana, Lucas Vinnicio de Araujo; Soares, Anísio Francisco; http://lattes.cnpq.br/9044747136928972; http://lattes.cnpq.br/9709966355454943In March 2020, the World Health Organization (WHO) declared the COVID-19 pandemic when the world had already reported 118,000 cases, 4.2 thousand deaths, and 114 countries with confirmed cases. With the lack of effective treatments and vaccines, scientists turned their attention to diagnosing the disease and finding ways to prevent its transmission. Although RT-PCR testing is widely used, it has some disadvantages, such as invasiveness of sample collection, cost, and time. Detection of volatile organic compounds (VOC) has emerged as a less invasive and effective alternative for COVID-19 diagnosis. Infection by a pathogenic agent can trigger a series of changes in the organism's cellular behavior. Understanding the molecules involved in this process is critical for developing diagnostic techniques that can help combat the pandemic. To investigate the relationship between COVID-19 and organic volatile biomarkers (VOC), a systematic review was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.Item Diagnóstico comparativo entre a histopatologia e a imuno-histoquímica de neoplasia maligna em membro pélvico de um canino da raça pastor de shetland - Relato de caso(2023-04-25) Silva, Aline Helena Albuquerque da; Oliveira, Andrea Alice da Fonseca; http://lattes.cnpq.br/2519790342576901; http://lattes.cnpq.br/4576494329697205The cases of neoplasms in pets show a significant growth compared to decades ago, being considered as one of the main causes, when malignant, of death or euthanasia among canines and felines. The use of existing diagnostic resources favors obtaining early results, positively impacting the survival of affected animals. Some neoplasms show a very marked degree of undifferentiation or anaplasia, requiring the adoption of immunohistochemistry (IHC) for diagnostic conclusion. The objective, with this case report, was to present a clinical case of neoplasm in the region of the interdigits of the left pelvic limb in a canine of the Shetland Shepherd breed, in which immunohistochemistry was necessary for diagnostic confirmation. The patient underwent surgical resection with amputation of the digit involved to reduce the chances of recurrence. IHC was requested for diagnostic confirmation after carrying out cytology and histopathology tests, which suggested malignant mesenchymal neoplasia and histiocytic sarcoma, respectively. The markers expressed by the cells ruled out the diagnostic conclusion of the histopathological examination. Therefore, it is concluded that immunohistochemistry is of great importance to confirm the type of neoplasia involved, preventing chemotherapy protocols from being erroneously adopted due to mistaken histopathological reports.Item Relatório de conclusão do programa de residência em área profissional de saúde em medicina veterinária - medicina veterinária preventiva - doenças parasitárias: inquérito soroepidemiológico para detecção de anticorpos anti-Trypanosoma cruzi em cães provenientes de Camaragibe, Pernambuco(2022-02-23) Lima, Maria de Lara Oliveira; Alves, Leucio Câmara; http://lattes.cnpq.br/6563157522654726; http://lattes.cnpq.br/6108177158055138The Residency Program in the Professional Health Area in Veterinary Medicine at the Federal Rural University of Pernambuco (UFRPE) was developed with the aim of preparing Veterinarians for the service in their specific area of knowledge. The program presents a lato sensu postgraduate teaching modality, with a full-time regime and duration of 24 months. This report aims to describe the theoretical-practical activities developed during the residency, from March 2020 to February 2022, totaling a workload of 5,760 hours, divided into two major areas of concentration, one specific and one common to all program professionals. The workload developed in the specific area of Preventive Veterinary Medicine - Parasitic Diseases, was 4,800 hours, dedicated to the routine of the Laboratory of Parasitic Diseases at UFRPE as well as the development of theoretical disciplines throughout the residency period, and 960 hours were developed in Public Health, distributed in the areas of Health Surveillance and Primary Health Care (NASF-AB) in the city of Recife, Pernambuco. Moreover, a research about “Seroepidemiological survey for detection of anti-Trypanosoma cruzi antibodies in dogs from Camaragibe, Pernambuco” was made.Item O transplante de células tronco hematopoiéticas como tratamento da anemia falciforme: uma revisão de literatura(2022-09-30) Oliveira, Luís Eduardo Rangel Batinga de; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/9122060791637391Item Relatório do Estágio Supervisionado Obrigatório (ESO) realizado no 4 Patas Hospital Veterinário, município de Olinda - PE, Brasil e Hospital Veterinário Harmonia, município de Recife - PE, Brasil. Alterações hematológicas de cães com inclusão citoplasmática viral patognomônica para cinomose(2021-03-03) Cavalcanti, Anna Carmen Rocha; Oliveira, Andrea Alice da Fonseca; http://lattes.cnpq.br/2519790342576901; http://lattes.cnpq.br/0903673289567984The mandatory supervised internship, last course of the Veterinary Medicine program, demonstrates to be a fundamental moment in a students life, since it brings a practical background in the desired field of work. In this regard, a whole professional practice is experienced, in order to prepare the newcomer into the labor market. Therefore, the report describes the activities performed in the veterinary clinical pathlogy field, in the 4 Patas Veterinary Hospital, under the supervision of the veterinarian Camila Coutinho; and in the Harmonia Veterinary Hospital under the supervision of the veterinarian Allison Macêdo. The total workload was 420 hours, divided into 210 hours per clinic, from August 28th till December 9th, 2020. The addressed data was obtained via laboratory test count. In addition, it is important to point out that a scientific paper was developed from data collection during the internship in the Harmonia Veterinary Hospital, in which blood tests reports from dogs with viral cytoplasmic inclusion bodies (Lentz bodies), pathognomonic to Canine Distemper, were analyzed and compared to the described literature, highlighting the importance of the total blood count, as well as the need of other laboratory tests of confirmatory nature. Thusly, it is concluded that, the mandatory supervised internship was essential in the learning of professional routine, establishing work relationships, exposure to different settings, understanding of existing dynamics in each setting and also, technical enhancement of theoretical-practical knowledge acquired during undergraduate studies.Item Inquérito coproparasitológico e ações de educação em saúde em escolas da rede pública de três municípios de Pernambuco(2019) Albuquerque, Maria Eduarda de; Oliveira, Jaqueline Bianque de; http://lattes.cnpq.br/2856383385211373; http://lattes.cnpq.br/0874155179625633Enteroparasites are a public health problem, since they are endemic in many regions of the country. Infections caused by intestinal parasites are considered socioeconomic indicators of the population and maybe associated with several determinants,suchas absence or precariousness of basic sanitation, contaminated food and water, and some sociocultural factors. In this context, the objective of this study was to identify the intestinal parasites of school children from the public schools of three municipalities of the state and to carry out health education actions to reduce contamination by these parasites. A total of 650 stool specimens of schoolchildren of both sexes and aged 3 to 15 years were analyzed from the public school system of the municipalities of Camocim de São Félix, Salgadinho and Tupanatinga, which were processed by sedimentation methods spontaneous and Kato Katz (Helm Test Bio-Manguinhos). Of the analyzed samples, the following intestinal parasites were diagnosed in 263 (40.46%): Ascaris lumbricoides (30,30%), Giardia duodenalis (4,61%), Entamoeba histolytica (2%), Hymenolepis nana , Schistosoma mansoni (0.30%), Hookworms (0.30%),Enterobiusvermicularis(0.30%)andTaeniasp.(0.15%).Theprevalencesin the municipalities studied were: Salgadinho 94,81%, Camocim de São Félix 15,41% and Tupanatinga 12,79%. In the urban area, the prevalence was higher (41.97%). Based on the results, actions were carried out to promote health through the production of materials and recreational activities aimedat the prevention of enteroparasitoses. In addition to health education activities, investments are needed to expand the coverage of basic sanitation and, thus, reduce the impact of intestinal parasites on the health of the population.Item Aplicação da citometria de fluxo no estudo da resposta imune de pacientes com Leishmaniose tegumentar americana(2019) Pereira, Allana Maria de Souza; Hernandes, Valéria Pereira; Oliveira, Beatriz Coutinho de; http://lattes.cnpq.br/1971181834355538; http://lattes.cnpq.br/9260270756078209; http://lattes.cnpq.br/0605182920865262The American tegumentary leishmaniasis (ATL) is an infectious, non-contagious disease with an average of 1.3 million annual cases. Brazil shows the highest prevalence in number of reported cases in the Americas, where the specific agent is the species L. (V.) braziliensis. The diagnosis is commonly performed by an association of clinical, epidemiological and laboratorial tests. The immunological approaches which are most commonly used in the routine are the indirect immunofluorescence (IFI) and the enzyme-linked immunosorbent assay (ELISA), however, they may present limitations. Therefore, alternative methods have been studied, and one of them is flow cytometry (FC). Thus, the aim of this work is to evaluate, by flow cytometry, the role of the humoral response in the development of cutaneous lesions of patients with the active disease before treatment (BT), and in those healed after therapeutic intervention (PT). Sera samples were inactivated. For the FC assays, promastigote forms of L. (V.) braziliensis obtained by the reference strain were expanded in Schneider’s medium until they reached exponential phase. After washing protocols, the concentration of the parasites was adjusted and they were fixed with 1% paraphormaldehyde. For the IFI and ELISA assays, the total antigen of L. (V.) braziliensis were used. In FC and IFI assays, the IgG antibody was conjugated to a fluorophore and in ELISA, to an enzyme. Regarding IFI results, from the evaluated sera, 92.85% (13/14) were positive on BT group, 61.54% (8/13) were positive 1 year PT, 70% (7/10) 2 years PT and 50% (5/10) 5 years PT were positive. In ELISA, 92.8% (13/14) of BT patients were positive; and 53.8% (7/13) 1 year PT, 88.8% (8/9) 2 years PT and 100% (5/5) 5 years PT (5/5) remained positive for ATL. In FC, 86% (12/14) of BT patients were positive and 77% (10/13), 80% (8/10) and 70% (7/10) of the patients 1, 2 and 5 PT, respectively, were negative for the assay. Based on the ROC curves analysis to compare the performance of the 3 tests, it was observed that the area under the curve (AUC) of IFI (AUC=0.879; IC95%= 0.754-0.954) was lower that FC’s (AUC= 0.890; IC95%=0.767-0.961), meaning it had a lower performance. Comparing ELISA and FC, it was observed that the AUC of ELISA (AUC=0.808; IC95%= 0.652-0.915) has differed from the one observed for FC (AUC= 0.896; IC95%= 0.758-0.970), where again, FC has shown a superior performance. As for the results of the IgG isotypes’ (IgG1, IgG2 and IgG3) standardization, it was observed that the best dilution for IgG1 was 1:400; for IgG2, 1:100; and for IgG3, 1:200. On the analysis to verify IgG1 applicability, it was seen that BT 63.2% of the patients were positive, and 1PT, 17.7%; 2PT, 72.8% and 5PT, 12.55% of the patients were positive. Therefore, we believe that FC can be used as a diagnostic tool for ATL since it is positive in the presence of the disease, being superior to the two conventional methods used in the laboratory routine. Also, the use of the IgG1 isotype has the ability to contribute as a more sensitive and specific diagnostic method when compared to the classical ones.