Bacharelado em Ciências Biológicas (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/5
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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3 resultados
Resultados da Pesquisa
Item Diversidade das aquaporinas no genoma de Culex quinquefasciatus: um estudo in silico(2023-09-19) Xavier, Italo Gois; Oliveira, Iêda Ferreira de; http://lattes.cnpq.br/5288071226008713; http://lattes.cnpq.br/3953245363870196Culex quinquefasciatus is a cosmopolitan species that transmits the nematode Wuchereria bancrofti, the etiologic agent of lymphatic filariasis. Throughout its life cycle, this mosquito needs to survive adverse environmental conditions and various biological stressors. In this process, molecular mechanisms are essential for reestablishing the body's homeostasis and aquaporins are a group of potentially important biomolecules in these responses. Aquaporins are a superfamily of highly hydrophobic, transmembrane channel-forming proteins that are present in all living organisms, primarily responsible for the facilitated transport of water. The objective of this work was to identify, classify and characterize the aquaporins present in the genome of the mosquito Culex quinquefasciatus using in silico tools. For this, comparative structural and functional analysis of the gene and protein sequences of the aquaporins identified in the NCBI database were carried out, using genomic information from the species Drosophila melanogaster, Anopheles gambiae and Aedes aegypti. Six probable genes and 15 isoforms have been identified in the Culex quinquefasciatus genome, all located on chromosome 2, and classified into the DRIP, BIB, PRIP, Eglp1 and Eglp3 subfamilies. All protein sequences were confirmed for topology, architecture, subcellular localization and other physicochemical configurations characteristic of aquaporins. The motifs and gene structure of aquaporins from Culex quinquefasciatus were conserved within the same family, but subtly different among members of different families, reflecting their different biological functions. The results of these analyzes may help to better understand the molecular aspects of these transmembrane transport proteins, which are of great importance for the biology and evolution of this species of medical and veterinary importance.Item Análise de colinearidade gênica do operon aprX-lipA em isolados de Pseudomonas fluorescens(2023-09-15) Silva, Israel Santos da; Freitas, Nara Suzy Aguiar de; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/6891650997818766; http://lattes.cnpq.br/9803828236017805Pseudomonas fluorescens are Gram-negative bacilli that are motile and found in terrestrial and aquatic ecosystems. Due to their psychrotrophic characteristics, these bacteria are often associated with the contamination of unpasteurized milk and its derivatives, such as cheese and butter. The proteases and lipases produced by P. fluorescens are the primary factors in the prevalence of dairy product contamination. These enzymes are encoded by the aprX and lipA genes, which are present in the aprX-lipA operon. In this regard, we evaluated the genomic components surrounding the aprX-lipA operon of P. fluorescens from different sources, aiming to detect genetic patterns inherent to these organisms and their correlation with proteolytic activity. We used the NCBI database, the String platform, and Interpro for comparative evaluation of the selected genomes. In the four isolates analyzed, the aprX-lipA operon is highly variable structurally, with unique configurations for each genome. The gene co-expression relationships of the genes surrounding the aprX protease also show qualitative and quantitative variations, both intra- and inter-species within the Pseudomonas genus. We detected components of the CRISPR type 1 system, previously unrelated to the operon, which can amplify, move, and modify genes related to the defense mechanism, pathogenic or not, of the genus. The patterns related to pathogenicity indicate that new biomarkers can be used for genomic surveillance.Item Perfis genômicos do transposon Tn4401 de isolados de Proteus sp., Providencia sp. e Morganella sp(2022-07-03) Silva, Larissa Almeida da; Freitas, Nara Suzy Aguiar de; Santos, Dayane da Silva; http://lattes.cnpq.br/0456504242112576; http://lattes.cnpq.br/6891650997818766Antibiotic resistance is a growing global threat to public health, causing high mortality. Mutation, genetic drift, horizontal gene transfer via transposons, and natural selection are the basic mechanisms responsible for the spread of resistance genes among bacterial populations. The Proteeae tribe is composed of three genera: Proteus spp., Morganella spp. and Providencia spp., opportunistic pathogenic bacteria that are resistant to [beta]-lactams. The highly mobile transposon Tn4401 from Klebsiella pneumoniae and Escherichia coli is characterized by containing blaKPC genes encoding [beta]-lactamase, ensuring resistance to β-lactams. The objective was to analyze the evolutionary history of the genomes of the Proteeae tribe and its Tn4401 transposons, through patterns, variations and syntenies of genome sequences and their neighboring regions. Initially, the sequences of K. pneumoniae Tn4401 (Kp15, Kp512, Kp529-1) were used as a query in BLASTn/NCBI to search for strains of the Proteeae tribe. Then the selected GenBank files were used in Islandviewer 4.0 software for similarity and synteny analysis and the FASTAS sequences were incorporated into ClustalW/MEGA 11. After alignment, the ratio non-synonymous for synonymous substitutions was established. Different isoforms of Tn4401 were revealed, between them Tn4401a, Tn4401b and Tn4401d containing blaKPC. Several patterns of gene sitenia of transposon were detected, involving deletions, inversions, duplications and mobility of neighboring gene groups, sometimes similar, sometimes different. Purifying selection was an active force in the β-lactamases of the analyzed strains. We also detected a possible relationship between the transposon isoforms and neighboring islands of resistance to Mercury, Chromate and Sulfonamide. These findings emphasize the continuous action of different evolutionary processes on plasmids and on the Tn4401 element, as well as the possible potential for dissemination of resistance genes by the Proteeae tribe.