01.1 - Graduação (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2
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Resultados da Pesquisa
Item Microbiota bacteriana associada a pele de Scinax x-signatus (Spix, 1824) (Anura: Hylidae) em Pernambuco, Brasil(2020-02-03) Santos, Lara Valesca Mendonça da Costa; Santos, Alcina Gabriela Maria Medeiros da Fonsêca; Moura, Geraldo Jorge Barbosa de; http://lattes.cnpq.br/1348666346504103; http://lattes.cnpq.br/4938571253882757; http://lattes.cnpq.br/6095901652697979The presence of a symbiotic microflora in extremely sensitive animals like amphibians is crucial for the development of an immune system capable of fighting the main opportunistic infectious agents in the environment. Scinax x- signatus is a anuro which has a general and synanthropic behavior occurring in forests and urban areas, but there are no identification studies of bacterial microbiota associated with skin of this animal. The objective of this study was to identify the skin microbiota of Scinax x-signatus in two areas in the Botanical Garden Reef (08 º 04’ S, 34 º 59’ W) and compare the resident and transient microorganisms. Rinses were performed with sterile distilled water to remove the animals from transient microflora and swabs wiped on the skin to collect the resident microflora. Samples were predictively you identify in selective media, as Chromogenic Agar, Cetremide, EMB Agar and MacConkey agar For the analyzes, we used Fisher Test and Cluster Analysis to verify the similarity between the collection areas. 24 sample were isolated from bacterial microflora and predictive methods were taxa 5: Pseudomonas spp. (43%) occurring in most samples, Escherichia coli (22%), Klebsiella pneumoniae (18%), Enterococcus faecalis (12%) and Staphylococcus aureus (4%), with the lower frequency. The microbiota from the anthropized area showed less similarity between the resident and transient samples. Cluster Analysis indicated that the microbiota from the control area was more similar among transients and residents samples, possibly due to the low degree of spatial heterogeneity. Scinax x-signatus specimes presented skin microorganisms of medical interest that are similar to other species of frogs. S. x-signatus has great ecological importance to ecosystems, and studies to research antimicrobial potential on the skin and in the microbiota associated with S. x-signatus are very important for the production of pharmaceuticals.Item Ocorrência e potencial simbiótico de populações de rizóbios nativas de solos do semiárido(2019-07-11) Oliveira, Andressa Silva de; Freitas, Ana Dolores Santiago de; http://lattes.cnpq.br/6734173724110965; http://lattes.cnpq.br/3758377236956687The use of nitrogen-fixing bacteria through the rhizobium-legume symbiosis is a sustainable baseline solution to reduce potential environmental impacts caused by the use of industrialized fertilizers and the burning of fossil fuels. For this, it is necessary to select efficient rhizobia adapted to the edaphoclimatic conditions of each region. Thus, this work aimed to evaluate the occurrence and symbiotic potential of the bean rhizobia populations naturally established in semi-arid soils. In order to capture the isolates, areas with dense caatinga cover (with little anthropic interference) and the main soil classes occurring in the biome were selected: Argissolo, Neolithic, Quartzarenic Neosol, Regolithic Neosol, Latosol, Luvisol and Planosol of different municipalities located in Agreste and Sertão de Pernambuco. A randomized block experiment with three replicates was conducted. The species used as plant-bait was cowpea (Vignaunguiculta (L.) Walp), BRS Pujante variety. Five nodules were randomly chosen from each replicate to proceed to isolation. After the isolation and growth of the isolated colonies, the morphophysiological characterization of the isolates was evaluated, evaluating the reaction of pH in culture medium, growth time and exopolysaccharide production. Bean Cowpea developed normally, presenting 100% survival. For nodulation, in all soil classes studied, populations of rhizobia capable of nodulating the crop were observed. 338, mostly fast growing, were included that acidify the culture medium and have a lot of mucus. The characteristics have been used for these noduliferous species of different species of legumes natives of semi-arid regions of Brazil.