TCC - Zootecnia (Sede)
URI permanente para esta coleçãohttps://arandu.ufrpe.br/handle/123456789/478
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Item Atividade antimicrobiana de óleos essenciais de orégano (Origanum vulgare), pimenta rosa (Schinus terebinthifolia) e melaleuca (Melaleuca alternifólia) frente aos patógenos de origem alimentar(2024-02-21) Silva, Esterfani Pereira da; Soares, Luciana Felizardo Pereira; http://lattes.cnpq.br/4071178363761831; http://lattes.cnpq.br/3144149210017027In recent years, the use of synthetic additives to prevent food spoilage by microorganisms has been a worrying factor for the food industry, given their negative impact on consumer health. In order to overcome this impasse, essential oils have emerged as an alternative, containing compounds and active principles that inhibit the development of microorganisms. The aim of this study was to evaluate the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the commercially obtained essential oils of Tea tree (Melaleuca alternifolia), Oregano (Origanum vulgare) and Pink pepper (Schinus terebinthifolia). For the study, bacterial isolates at a concentration of 3.5 x 107 CFU/ml (0.5 on the Mc Farland scale) of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) obtained commercially from the American Type Culture Collection were used. The incubated plates were visually analyzed to determine the minimum inhibitory concentration (MIC). In the wells where inhibition of bacterial growth was observed, 20 μL of the suspensions were collected and sown on Mueller-Hinton agar plates in an oven at 37ºC for 24 hours to analyze bacterial growth and determine the minimum bactericidal concentration (MBC). The mean MIC values for oregano, pink pepper and tea tree essential oils were 2500 μg/ml, 650 μg/ml and 500 μg/ml for the E. coli strains, respectively, while the minimum inhibitory concentration of the treatments was 500 μg/ml for the S. aureus bacterial group. As far as CBM is concerned, the average concentrations for the essential oils of oregano, pink pepper and tea tree were 2500 μg/ml, 625 μg/ml and 5000 μg/ml for the E. coli strains and 1250 μg/ml, 2500 μg/ml and 625 μg/ml for the gram-positive bacteria S. aureus, respectively.Item Digestibilidade de nutrientes de dietas de aves poedeiras na fase de cria alimentadas com aditivo simbiótico(2021-07-06) Andrade, Mariane Farias de; Rabello, Carlos Bôa Viagem; http://lattes.cnpq.br/4760288746238700; http://lattes.cnpq.br/4027425613630492The objective was to determine the effect of the use of symbiotic additive on the energy and nutrient utilization of layer feed in the rearing phase. The study was conducted in the laboratory of poultry research (LAPAVE) of the Department of Animal Science of the Federal Rural University of Pernambuco, with a duration of 35 days. A total of 1040 chicks of the Dekalb White strain from 1 to 5 weeks of age were used, distributed in an entirely randomized design with 4 treatments, 10 repetitions with 26 birds each. The treatments were composed of two base diets: the first composed of corn and soybean meal, with no feed additives - RR; the second control diet composed of corn, soybean meal and meat and bone meal, with no feed additives - FCO; to formulate the third diet, 0.05% of the additive Bacitracin Zinc - Bac Zn was added to the FCO diet, and 0.1% of the symbiotic additive - Simb-C was added to the FCO diet for the fourth diet. For nutrient digestibility evaluation, the collection of excreta was performed at the 3rd week of age of the birds, following the protocol of partial collection method, in which 1% of Celite® indicator was introduced in the diets, in order to measure nutrient digestibility. The collection of excreta occurred three consecutive days, preceded by three days of adaptation to the diet with the indicator. The collected excreta were packed in plastic bags and frozen in a freezer to perform the analyses and calculations for determining nutrient digestibility (dry matter and crude protein) and gross and metabolizable energy values of the diets. Thus, three contrasts were generated, consisting of: Contrast 1: RR vs FCO; Contrast 2: FCO vs Bac Zn; Contrast 3: Bac Zn vs Simb-C. The results obtained showed that there were no significant differences between the variables CMAMS, CMAPB, CMAEB, EMA and EMAn in the contrast of the RR vs FCO diets. However, when contrasting the FCO vs. Bac Zn diets, the MCPAB was better in the diet containing the zinc bacitracin additive, which had lower values than the other variables. However, the diet containing FCO showed a better use of CMAEB, EMA and EMAn. When evaluating Bac Zn vs Simb-C no significant differences were observed between MCAPB but for the other variables the symbiotic provided lower values. It can be concluded that the symbiotic additive proved to be a possible substitute for the antibiotic zinc bacitracin in layer feed during rearing.Item Efeito da adição de zeólita natural (Clinoptilolita) em dietas sobre os parâmetros sanguíneos de poedeiras(2022-10-07) Santos, Adrielle Carneiro de Araújo; Ludke, Maria do Carmo Mohaupt Marques; http://lattes.cnpq.br/4629657233206289; http://lattes.cnpq.br/5809137471735648The objective of this work is to evaluate the effect of the addition of natural zeolite (Clinoptilolite) in diets on the serum and hematological biochemical profile of laying hens. The treatments consisted of six levels of zeolite inclusion, 0, 0.7; 1.4; 2.1; 2.8; 3.5%, where all diets were isoenergetic and isoproteic, differing only the replacement of an inert material (washed sand) by the zeolite additive. The design was in randomized blocks, with 8 replications and 5 birds per plot, totaling 240 ISA-Brown laying hens from the 54th week with approximate weight of 1.75 kg. To perform the hematological analysis, 1 bird per plot was used and 4ml blood samples were collected from the ulnar vein of one of the wings. The hematological variables were: red blood cells, hemoglobin, platelets, leukocytes, total plasma proteins, heterophils, lymphocytes, eosinophils and monocytes. To evaluate the biochemical parameters, samples of 4ml of blood each were collected from 2 birds per plot and submitted to analysis of total proteins, albumin, calcium, creatinine, urate/uric acid, urea, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma glutamyl transferase (GGT). Data were submitted to analysis of variance, and if significant, regression analysis and Dunnet's test at 5% probability were performed. No significant effect was observed for the biochemical parameters. As for the hematological variables, an effect of zeolite on total lymphocytes was observed (p=0.0006). When the regression analysis was performed, a quadratic effect was found (p=0.0035). When applying Dunnet's test, it was found that the absolute value of lymphocytes differed as a function of natural zeolite (Clinoptilolite) in relation to the control diet at the level of 3.5% inclusion. With this, it is concluded that clinoptilolite can be used in the feeding of laying birds at the level of 3.5% of inclusion, providing a greater amount of lymphocytes, stimulating the production of antibodies and improving immunity.Item Efeito da inclusão de farinha de minhoca com e sem zeólita natural na dieta de tilápia (Oreochromis niloticus) sobre os parâmetros de carcaça(2022-10-07) Oliveira, Elton Francisco de; Ludke, Maria do Carmo Mohaupt Marques; http://lattes.cnpq.br/4629657233206289; http://lattes.cnpq.br/3930176884309812The purpose of this research is to evaluate the effect of the addition of earthworm flour and the inclusion of zeolite (clinoptilolite) on carcass composition parameters. In the experiment, 280 tilapia of the species Oreocrhomis niloticus were used, with an initial and final weight of 2.826±0.0896g and 13.59±0.75g, respectively. The treatments were 8 diets corresponding to a 4 x 2 factorial arrangement, with 4 levels of inclusion of earthworm flour (0, 3.5; 7.0 and 10.5%) and levels of 0 and 2% of zeolite (Clinoptilolite ), the fish were distributed in a randomized block design with 5 replications. The following parameters were evaluated: carcass yield (RC), carcass dry matter (MSC); carcass organic matter (MOC); Mineral Substrate Matter (MMC); carcass energy based on dry matter (EMSC); ethereal substrate extract (EEC); energy retention coefficient (CRE); crude carcass protein (CBP); RE= Energy retention coefficient; CRP= Protein retention coefficient; TEP= Protein Efficiency Rate; IHE= Hepatosomatic Index; IVS= Viscerosomatic Index; At the end of the experiment (85 days) the animals were stunned with eugenol and then sacrificed and frozen, later they were lyophilized for analysis. The data were submitted to analysis of variance, in order to observe the main effects and the interaction between the factors (Worm meal levels and Zeolite inclusion) on the composition and protein and energy retention in the fish body. All analyzes were performed using the GLM procedure of the SAS computational package, at a 5.0% significance level. According to the data, there was a significant reduction in the amount of lipdis (p=0.0208) and in the energy retention coefficient (CRE) (p=0.0376) in the carcass of these animals when adding Zeolite in the diets. As for earthworm meal, a significant effect (p=0.0500) on CRE was observed, where when performing the regression there was a linear increase (P=0.0188) when including this ingredient in the diets of these fish. With this, we can add up to 10.5% of earthworm meal in diets for Tilapia, however Zeolite reduced energy efficiency, not interfering with other carcassItem Efeito da utilização de diferentes promotores de crescimento sobre o desempenho de suínos nas fases de pós-desmame(2021-12-01) Souza, Andréa Silva Marques de; Lima, Tayara Soares de; http://lattes.cnpq.br/3100045021780173; http://lattes.cnpq.br/5272839332691842The objective of this work was to evaluate the effect of different types of growth promoters in the diet of pigs in nursery phase. The performance test was conducted in the Pig Farming sector of the Animal Science Department of the Federal Rural University of Pernambuco. The handling and treatment of the animals followed the guidelines of the Ethics Committee on the Use of Experimental Animals, under license number 6272191120. A total of 48 weanling pigs, from 25 to 81 days of age, were used in a randomized block design, with four treatments and six blocks representing the repetitions, the blocking factor used was the piglet weight. The treatments consisted of a reference diet based on corn and soy, without addition of antimicrobial (CO), a reference diet with the addition of antimicrobials Zinc Bacitracin/Florphenicol/Enrofloxacin (C+A), a reference diet with addition of 0 .5% organic acids (AO) and a reference diet with addition of moringa seed oil (Basal + 500ppm) (OM). For performance evaluation, daily feed intake (CRD), daily weight gain (GPD) and feed conversion (CA) were evaluated. Data were submitted to normality, homoscedasticity, and analysis of variance tests by the PROC MIXED of SAS. Duncan's test was used for the performance means, it was observed that there was no statistical difference between the promoters for the different variables tested and due to the lack of sanitary challenge in the present study, it was concluded that the addition of any would not be necessary. of these additives in the diet of the animals, since the animals that received the control diet showed the same performance as the supplemented animals.Item Qualidade de ovos armazenados à temperatura ambiente de poedeiras alimentadas com dietas contendo simbiótico(2023-04-21) Siqueira, Monique Aguiar; Rabello, Carlos Bôa Viagem; http://lattes.cnpq.br/4760288746238700; http://lattes.cnpq.br/3658833045052053The study evaluated the effects of using the symbiotic in the diet of laying hens from the rearing, rearing or production phase, replacing zinc bacitracin on the quality of eggs produced in the post-peak phase stored at room temperature. 198 birds of the Dekalb White strain, aged 70 weeks, evaluated up to 90 weeks were used. For the evaluation of egg quality, the experimental design used was completely randomized in a 6 x 3 factorial scheme, with 6 experimental diets and 3 storage periods, determined at 0, 7 and 14 days, with 6 replications. The experimental diets consisted of a diet composed of corn and soybean meal without additives (RR); diet composed of corn, soybean meal and including meat and bone meal without additives (MBM); and four more rations based on the MBM diet with the addition of additives, namely, a diet with the addition of 0.05% of the Zinc Bacitracin additive from the start phase (MBM+Bac Zn); diet with the addition of 0.1% of the Symbiotic additive from the start phase (MBM+Simb-SF); diet based on MBM with the addition of 0.1% of the Symbiotic additive from the grower phase (MBM+Simb-GF), and the last diet based on MBM with the addition of 0.1% of the symbiotic additive from the production phase (MBM+Simb-PF). For the quality evaluation, 108 eggs were collected from birds at 80 weeks of age, shortly after laying. were submitted to the analysis of variance. After finding significance, the data were submitted to the Tukey test (p<0.05) and analyzed using the mixed model, using the SAS statistical package. The diets significantly influenced the variable yolk color, where the treatment with zinc bacitracin obtained the highest value, and albumen pH, influenced by the addition of MBM. On the other hand, all variables were influenced by storage time, indicating that prolonged storage periods contribute to a decrease in egg quality, regardless of the type of additive or the period of use. Thus, the symbiotic additive can be used as an alternative to zinc bacitracin because it presents results, about egg quality, similar to those where the antibiotic additive is used, except for the color intensity of the yolk. Also, the additives used, regardless of the inclusion phase, were not able to reduce the effects of storage time on egg quality.