01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Item Microrganismos fotossintetizantes como potencial fonte de moléculas bioativas contra Leishmania spp.: uma revisão(2023-08-14) Silva, Sabrina Swan Souza da; Bezerra, Raquel Pedrosa; Andrade, Alexsandra Frazão de; http://lattes.cnpq.br/8560904255362766; http://lattes.cnpq.br/1466206759539320; http://lattes.cnpq.br/8504258200413633Leishmaniasis are infectious diseases caused by protozoan parasites of the genus Leishmania and represent a serious public health problem with global impact, affecting thousands of people every day. The drugs currently available for treatment are based on pentavalent antimonials that have adverse side effects with cases of resistance and ineffectiveness being frequently reported. Thus, photosynthetic microorganisms (microalgae and cyanobacteria) are a wide source of compounds that can be used in the treatment of various diseases, and due to their rapid growth combined with their minimum nutritional requirements. they have a reduced production cost, making them strong candidates as raw material for the development of new medicines. The search was conducted in databases such as Google Scholar, ScienceDirect, National Center for Biotechnology Infozmation (NCBI) and Virtual Health Library (VHL) using the terms alone and in combination to identify the articles: cyanobacteria, microalgae, photosynthetic microorganisms, bioactives, antileishmanial, antiprotozoal. The inclusion criteria for this review were original articles describing the antiparasitic activity of extracts or compounds isolated from cyanobacteria and microalgae against Leishmania infection. Bibliographical references of the included articles were checked to identify possible eligible articles. In total, eleven articles were selected and analyzed based on the species of microorganisms, bioactive compounds, and their minimum concentration to reduce 50% of the parasite population (IC5o). Cyanobacteria were the most studied group, with an emphasis on the genus Lyngbya, while there was only one study using three genera of microalgae (Nannochloris spp., Picochlorum sp. and Desmochloris sp.). The in vitro studies found reported the use of peptides as the main bioactive with anti-Leishmania activity, with the ticonamide A peptide, which presented the lowest ICso value (1.14 FM), and the almiramide B and almiramide C peptides, the most selective for the parasite, with IS values of 21.7 and 17.4, respectively. Therefore, peptides from photosynthetic microorganisms are a promising source for the development of future pharmacological products against Leishmania.Item Atividade tripanocida de compostos bioativos oriundos de algas e cianobactérias: uma revisão(2021-12-07) Moura, Yanara Alessandra Santana; Marques, Daniela de Araújo Viana; http://lattes.cnpq.br/0788548123321981; http://lattes.cnpq.br/1018762976201930Chagas disease (CD), classified as a neglected tropical disease second to World Health Organization, is caused by the parasite Trypanosoma cruzi, which can assume two evolutive forms in the human host: trypomastigote and amastigote. DC possesses two clinical phases, the acute phase (generally asymptomatic) and the chronic phase (which can affect the cardiovascular, digestive and/or nervous systems). The currently available drugs to treat CD, benznidazole (BZN) and nifurtimox (NFX), are known for their high toxic profile, especially NFX. BZN, the first-line treatment, possesses limited efficacy in the chronic phase of CD and mechanisms of resistance had been associated to it. Thus, new compounds with trypanocidal activity are necessary to act as an alternative treatment against CD. Natural compounds are described as promising alternative for antiparasitic treatment. Algae and cyanobacteria are promising sources of bioactive compounds since they perform various biological activities reported in the literature, including antiparasitic activity. Therefore, this work aimed to make a review exploiting the antiparasitic activity of the biocompounds from algae and cyanobacteria against both trypomastigote and amastigote forms of T. cruzi. For this, it was conducted a search in Science Direct, Medline (PubMed), Web of Science (WoS), Springer Link, Wiley Online Library, Scielo, and MDPI. In total, 15 studies were included and the Rhodophyta and Ochrophyta phyla are the more studied and more promising for anti-T cruzi activity. The macroalgae Dictyota dichotoma and Ulva lactuca were the most studied. However, the highest antiparasitic activity was presented by the extract from macroalgae Stypopodium zonale, based on the extract concentration that can inhibit the parasites by 50% (IC50), while the species Tetraselmis suecica and Nostoc commune exhibited the best IC50 values among microalgae and cyanobacteria, respectively. Among all studies, only four active principles were identified, being elatol compound from Laurencia dendroidea the most promising based on its SI values for trypomastigotes (SI = 19.56) and amastigotes (SI = 26.73). The only in vivo study showed that microalgae Arthrospira maxima can be effective in T. cruzi-infected mice as treatment or prophylaxis. Although anti-T. cruzi activity of algal and cyanobacterial biocompounds is promising, further studies should exploit the mechanisms of action of the compounds, as well as new in vivo studies are required to make feasible future applications of these compounds for clinical trials in the treatment of CD.Item Diferentes respostas de espécies fitoplanctônicas aos efeitos da interação com Ceratophyllum demersum L. e herbivoria por Moina micrura Kurz, 1875(2019-12-03) Souza, Vitor Ricardo de; Moura, Ariadne do Nascimento; http://lattes.cnpq.br/5127314582444598; http://lattes.cnpq.br/1478768266839775The present work aims to understand the indirect allelopathic effects of Ceratophyllum demersum and the herbivory of Moina micrura on Microcystis aeruginosa and Raphidocelis subcapitata. To carry out the study, the algae strains were grown in 1000 mL erlenmeyers, filled with 800 mL of ASM1 medium, and under controlled temperature and light conditions. Two parallel experiments, lasting six days, were carried out in an aseptic room. The first experiment consisted of four treatments with triples, which evaluated the interaction of C. demersum + strains, M. micrura + strains, C. demersum and M. micrura + strains, and the control (strains in proportions equal 1:1). The second experiment had five treatments with triples, where the algae were coexisted under different concentrations: 1:0, 3:1, 1:1, 1:3 and 0:1. 2 mL aliquots were collected every two days (0, 2, 4 and 6) to determine density and subsequently obtain biomass. The data were treated statistically through ANOVA one-way with a significance level set at 5% (p> 0.05). Initially, the data were tested for normality predicted by the Kolmogorov-Smirnov test. In experiment 1, C. demersum and C. demersum + M. micrura significantly inhibited M. aeruginosa from the second day (p <0.01), whereas R. subcapitata did not show any differences during the whole experiment (p> 0.05). In treatments with only M. micrura, there was no change in the density of the strains (p> 0.05). Possibly C. demersum inhibited the growth of cyanobacteria through the release of allelochemicals, but chlorophyte was less affected by having greater physiological resistance. In experiment 2, M. aeruginosa did not inhibit the growth of R. subcapitata in equal (1:1) and dominant (3:1) proportions, but was inhibited by Raphidocelis in low concentrations (1:3). It is possible that chlorophyte is stimulated when competing algae are at lower densities. We conclude that the action of C. demersum is efficient in the control of M. aeruginosa, differently from M. micrura. At low concentrations, M. aeruginosa can be inhibited by R. subcapitata, while R. subcapitata is possibly to resist both the macrophyte allelochemicals and the cyanobacterial microcystins.Item Sensibilidade de cepas de Microcystis submetidas à coexistência com Ceratophyllum demerssum L.(2019-09-18) Falcão, Rafael Henrique de Moura; Moura, Ariadne do Nascimento; http://lattes.cnpq.br/5127314582444598; http://lattes.cnpq.br/2866001009624251