01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)

URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1

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    Caracterização molecular de determinantes de resistência a antibióticos de Salmonella spp. isoladas de carne de frango in natura comercializados em mercados públicos de Recife - PE
    (2020-02-03) Silva, Marcela Oliveira da; Souza, Paula Mariana Salgueiro de; http://lattes.cnpq.br/6281410502740086; http://lattes.cnpq.br/9549169192404311
    Salmonella is one of the main causative agents of foodborne infections. Salmonellosis becomes more dangerous when strains resistant to various antibiotics are found in food, especially in chickens, one of the main vehicles for transmitting this pathogen to humans. The aim of this study was to characterize the determinants of antibiotic resistance present in Salmonella spp. isolated from fresh chicken meat sold in public markets in the city of Recife. For that, 21 isolates of Salmonella spp. underwent phenotypic and molecular tests to assess susceptibility to antibiotics and to investigate determinants of resistance to [beta]-lactams and quinolones. The results obtained demonstrated the presence of three large clonal groups among bacterial isolates, in addition to the presence of genes encoding [beta]-lactamases and genes that confer resistance to quinolones. The spread of different polyclonal groups of Salmonella spp. MDR, in chicken carcasses, shown in this study, reinforces the need for effective controls to contain this microorganism, which in addition to being a risk to public health, is also responsible for considerable economic losses.
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    Atendimento às normas do Ministério da Agricultura para a conservação de carne de frango resfriada, nos pontos de venda em Recife - PE
    (2021) Silva, Bernadete de Lourdes Viégas da; Pires, Edleide Maria Freitas; Barreto, Larissa Santana; http://lattes.cnpq.br/2823183610144405; http://lattes.cnpq.br/0468713660747009
    The chicken meat production in Brazil surpasses 13 million tons, where 31% are exported and 69% supply the internal market, guaranteeing an annual consumption of 45.27 kilos of chicken meat per inhabitant. Its consumption is linked to several factors, such as being healthier, flavor, and lower price. This study aims at verifying if the selling of chicken meat in supermarkets of Recife is being practiced following the commercialization standards of current legislation. This work verified if the temperature where the meat is exposed is following the conditions presented by the legislation. We also present suggestions intended to improve the current conditions of storage, exposition, and selling of this product. It has been selected a sample of five sale points, where was collected six pieces of beef from each supermarket. It has been considered two types of cuts (chicken breast and chicken thigh). Hence, 30 meat samples in total. The samples were conditioned in polyethylene plastic bags or polystyrene boxes, simulating the same conditions the product is offered to regular customers. To evaluate the temperature conditions, a portable multi-function digital thermometer was used with a metallic rod and -50ºC to 300ºC measurement capacity, and 0,1ºC resolution. All of the five sites had visible thermometers and exposed enough pieces in the glass case. However, in one of the sites, the device presented a biased temperature. Four out of five sites had the correct number of pieces allowed to be exposed simultaneously. Regarding the temperature conditions of the chicken meat, only two sites have followed the regulations of the “regulatory standard n° 4/2014 – DIVISA/SVS/SES”. Therefore, only 13 out of the 30 samples (43%) have followed the current regulations of ideal temperature for refrigerated chicken meat. Therefore, approximately 57% of the visited places followed the regulation standards.The results allowed us to identify that especially the smaller stores face struggles to meet the regulations for accommodation and the ideal temperature of chicken meat, which impact the quality of the product for end consumers. It has been suggested the deployment of training programs to employees so that the quality and best-practices of the selling of chicken meat and its nutritional value can be kept. In consequence, improving the food safety for the clients.