01. Universidade Federal Rural de Pernambuco - UFRPE (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/1
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Resultados da Pesquisa
Item Caracterização da composição do plâncton em viveiros de piscicultura na Estação de Piscicultura Continental Johei Koike (UFRPE)(2022-10-07) Laurentino, Isla Mishele da Silva; Porto Neto, Fernando de Figueiredo; http://lattes.cnpq.br/1475750525654086; http://lattes.cnpq.br/3476650402323599An aquatic environment for the rational creation of fish has its own complex ecology, where the dynamics of biological and physical-chemical processes determine the conditions of water quality. Planktonic communities represent a sensitive tool to monitor environmental variations, such as indicators of water quality, levels of pollution and eutrophication, as well as important food sources for the trophic chain. The objective was to investigate the planktonic community of three active fish ponds in a diversion system and identify patterns in this community. Weekly water collections were carried out in three active fish ponds, at the Professor Johei Koike Aquaculture Station located on the headquarters of the Federal Rural University of Pernambuco (UFRPE). A superficial horizontal trawl was performed in each pond, with a plankton net with a mesh opening of 64 μm. The plankton samples were preserved in 70% alcohol and analyzed at the Zooplankton Laboratory of the Department of Oceanography at UFPE. The identification took place at the taxonomic level of large groups. For the three ponds, the water transparency was always below 25cm, decreasing from ponds 1 to 3 and in general the temperature was always between 28 and 29°C. Thus, a low variety of plankton species was observed in the ponds. Phytoplankton populations did not show great variations, in the three ponds the phylum Chlorophyta was the group of algae with the highest number of cells. On the other hand, zooplankton varied between ponds according to the type of fish cultivated and trophic conditions, the phylum Rotifera was in greater number in the three ponds, however, with abundance in relation to the other species in pond 3. that water quality parameters and the type of fish farmed appear to be the main factors controlling plankton populations in ponds.Item Extração e caracterização de extratos lipídicos obtidos da biomassa de Tetradesmus (Scenedesmus) obliquus com propriedades antimicrobianas(2021-07-15) Ferreira, Millena Patrício do Nascimento; Bezerra, Raquel Pedrosa; http://lattes.cnpq.br/1466206759539320; http://lattes.cnpq.br/1713734083159044Microalgae have become a source promising renewable energy, in addition it provides bioactive substances such as carbohydrates, proteins, vitamins and lipids. Lipids perform several biological functions and are fundamental to the survival of most living beings. Extraction is an important step in the process of obtaining lipids from microalgal biomass and can be performed in different ways in order to achieve greater yield and cost-benefit. This study performed the extraction and characterization of the lipid extract from the biomass of Tetradesmus obliquus and evaluated its antimicrobial property. Microalgae was cultivated in Erlenmeyers flasks for 15 days until it reached the stationary phase of cell growth. The dry biomass remained in contact with the solvents hexane, ethyl acetate and ethanol P.A, all with different polarities, for 48 hours in which the lipid yield and the fatty acid profile were determined by gas chromatography. Extracts of hexane (EHT), ethyl acetate (EAT) and ethanol (EET) were subjected to antimicrobial activity at concentrations of 100, 50, 25, 12.5, 6.25 and 3.12 mg/mL against bacteria Staphylococcus aureus, Klebsiella pneumoniae e Escherichia coli. EET obtained the highest lipid yield with 1.49±0.01% in the first extraction, while EAT obtained 1.22±0.02% and EHT yielded 0.52±0.03%. In a second extraction EHT doubled the yield with 1.17±0.00% and EAT managed to reach 2.47±0.00%, presenting better results with an reextraction. The essential fatty acids most present in the extracts were α-linolenic (15.4% to 19.9%) and oleic acid (5.74% to 8.93%), which are most present in the extract EHT and EAT. EAT managed to achieve better results by inhibiting the growth of all bacteria to the lowest concentration. EHT was positive until the concentration of 6.25 mg/mL and EET only managed to totally inhibit the growth of the E.coli bacteria at the concentration of 100 mg/mL. So far, it can be concluded that the ethyl acetate solvent is more suitable for extraction of T. obliquus lipids with antimicrobial activity.