Bacharelado em Ciências Biológicas (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/5
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APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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Resultados da Pesquisa
Item Diagnóstico coproparasitológico e avaliação da resistência anti-helmíntica da helmintose gastrintestinal em pequenos ruminantes(2021-09-30) Medeiros, Anderson Eduardo Correia; Alves, Leucio Câmara; http://lattes.cnpq.br/6563157522654726; http://lattes.cnpq.br/8784589514336726Gastrointestinal helminthiasis in small ruminants, especially those caused by nematodes, are frequent and require treatment with anthelmintics. Some factors such as management, inadequate treatment and underdosage can lead to the development of anthelmintic resistance (AR), representing a global problem. The benzimidazole (BZ) class is one of the most widely used in the treatment of helminthiasis and consequently one of the most common with resistance problems. One of the parasites most associated with AR in small ruminants is Haemonchus contortus, which causes serious problems in the herd and can lead to the death of the animal. Therefore, the aim of this study was to perform the coproparasitological diagnosis of small ruminants and detect molecular markers associated with resistance to BZs in populations of gastrointestinal nematodes of small ruminants in the state of Pernambuco. Feces were collected from 73 goats and three sheep from three farms and analyzed using the FLOTAC parasitological technique to obtain eggs per gram of feces (EPG). In addition, the abomasum content of 40 sheep was collected for adult helminth screening. A pool of feces was collected per farm and stool culture was performed to obtain infective third-stage larvae (L3). Adult helminthes and L3 were subjected to DNA extraction and polymerase chain reaction (PCR) for species identification and confirmation. The resistance profile of these parasites was determined by PCR amplification for the beta-tubulin gene related to AR, followed by sequencing. As a result, in FLOTAC the overall average was 1,797 Strongyloidea eggs. In stool cultures, L3 of Haemonchus sp. were the most frequently found, followed by Trichostrongylus spp. In the collection of abomasal contents, 15/40 (37,5%) of the animals presented the nematode. The PCR result of L3 and adult helminths confirmed the presence of H. contortus. However, no point mutations associated with BZs were found in the codons that have already been reported in H. contortus. Knowledge of anthelmintic resistance in nematodes present in small ruminants is essential to mitigate the development and dissemination of resistant isolates in Pernambuco.Item Inquérito coproparasitológico e ações de educação em saúde em escolas da rede pública de três municípios de Pernambuco(2019) Albuquerque, Maria Eduarda de; Oliveira, Jaqueline Bianque de; http://lattes.cnpq.br/2856383385211373; http://lattes.cnpq.br/0874155179625633Enteroparasites are a public health problem, since they are endemic in many regions of the country. Infections caused by intestinal parasites are considered socioeconomic indicators of the population and maybe associated with several determinants,suchas absence or precariousness of basic sanitation, contaminated food and water, and some sociocultural factors. In this context, the objective of this study was to identify the intestinal parasites of school children from the public schools of three municipalities of the state and to carry out health education actions to reduce contamination by these parasites. A total of 650 stool specimens of schoolchildren of both sexes and aged 3 to 15 years were analyzed from the public school system of the municipalities of Camocim de São Félix, Salgadinho and Tupanatinga, which were processed by sedimentation methods spontaneous and Kato Katz (Helm Test Bio-Manguinhos). Of the analyzed samples, the following intestinal parasites were diagnosed in 263 (40.46%): Ascaris lumbricoides (30,30%), Giardia duodenalis (4,61%), Entamoeba histolytica (2%), Hymenolepis nana , Schistosoma mansoni (0.30%), Hookworms (0.30%),Enterobiusvermicularis(0.30%)andTaeniasp.(0.15%).Theprevalencesin the municipalities studied were: Salgadinho 94,81%, Camocim de São Félix 15,41% and Tupanatinga 12,79%. In the urban area, the prevalence was higher (41.97%). Based on the results, actions were carried out to promote health through the production of materials and recreational activities aimedat the prevention of enteroparasitoses. In addition to health education activities, investments are needed to expand the coverage of basic sanitation and, thus, reduce the impact of intestinal parasites on the health of the population.Item Contaminação ambiental por ovos de helmintos em fezes de cães na orla da praia de Olinda, município de Olinda-PE(2019) Anjos, Larissa Maria Rabelo dos; Faustino, Maria Aparecida da Gloria; http://lattes.cnpq.br/6564907938376076; http://lattes.cnpq.br/9224485187600259Most parasitic helminths present an evolutionary cycle in which they are expelled to the outside, together with the feces, causing, thus, the contamination in the soil and its dissemination in the environment, being the animals like the dogs and cats, being sources of contamination of the soil, and thus man ends up being the target, since they use the same environments as animals. The objective of this study was to investigate environmental contamination by helmintheggs, with zoonotic potential, in faeces of dogs on the shore of the city of Olinda-PE. Fecal samples collected fromsidewalks on the beaches of Farol, Bairro Novo, Casa Caiada and Rio Doce were submitted to parasitological examination using the methods of Willis, Spontaneous Sedimentation and Flotac. It was observed that 41% of positivity for eggs of helminths, predominating, Ancylostomaspp. The Willis technique presented better egg detection performance.