Bacharelado em Ciências Biológicas (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/5
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
Navegar
3 resultados
Resultados da Pesquisa
Item Componentes orgânicos voláteis (COV) como biomarcadores da COVID-19(2023-04-28) Santana, Lucas Vinnicio de Araujo; Soares, Anísio Francisco; http://lattes.cnpq.br/9044747136928972; http://lattes.cnpq.br/9709966355454943In March 2020, the World Health Organization (WHO) declared the COVID-19 pandemic when the world had already reported 118,000 cases, 4.2 thousand deaths, and 114 countries with confirmed cases. With the lack of effective treatments and vaccines, scientists turned their attention to diagnosing the disease and finding ways to prevent its transmission. Although RT-PCR testing is widely used, it has some disadvantages, such as invasiveness of sample collection, cost, and time. Detection of volatile organic compounds (VOC) has emerged as a less invasive and effective alternative for COVID-19 diagnosis. Infection by a pathogenic agent can trigger a series of changes in the organism's cellular behavior. Understanding the molecules involved in this process is critical for developing diagnostic techniques that can help combat the pandemic. To investigate the relationship between COVID-19 and organic volatile biomarkers (VOC), a systematic review was conducted based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.Item Inquérito coproparasitológico e ações de educação em saúde em escolas da rede pública de três municípios de Pernambuco(2019) Albuquerque, Maria Eduarda de; Oliveira, Jaqueline Bianque de; http://lattes.cnpq.br/2856383385211373; http://lattes.cnpq.br/0874155179625633Enteroparasites are a public health problem, since they are endemic in many regions of the country. Infections caused by intestinal parasites are considered socioeconomic indicators of the population and maybe associated with several determinants,suchas absence or precariousness of basic sanitation, contaminated food and water, and some sociocultural factors. In this context, the objective of this study was to identify the intestinal parasites of school children from the public schools of three municipalities of the state and to carry out health education actions to reduce contamination by these parasites. A total of 650 stool specimens of schoolchildren of both sexes and aged 3 to 15 years were analyzed from the public school system of the municipalities of Camocim de São Félix, Salgadinho and Tupanatinga, which were processed by sedimentation methods spontaneous and Kato Katz (Helm Test Bio-Manguinhos). Of the analyzed samples, the following intestinal parasites were diagnosed in 263 (40.46%): Ascaris lumbricoides (30,30%), Giardia duodenalis (4,61%), Entamoeba histolytica (2%), Hymenolepis nana , Schistosoma mansoni (0.30%), Hookworms (0.30%),Enterobiusvermicularis(0.30%)andTaeniasp.(0.15%).Theprevalencesin the municipalities studied were: Salgadinho 94,81%, Camocim de São Félix 15,41% and Tupanatinga 12,79%. In the urban area, the prevalence was higher (41.97%). Based on the results, actions were carried out to promote health through the production of materials and recreational activities aimedat the prevention of enteroparasitoses. In addition to health education activities, investments are needed to expand the coverage of basic sanitation and, thus, reduce the impact of intestinal parasites on the health of the population.Item Aplicação da citometria de fluxo no estudo da resposta imune de pacientes com Leishmaniose tegumentar americana(2019) Pereira, Allana Maria de Souza; Hernandes, Valéria Pereira; Oliveira, Beatriz Coutinho de; http://lattes.cnpq.br/1971181834355538; http://lattes.cnpq.br/9260270756078209; http://lattes.cnpq.br/0605182920865262The American tegumentary leishmaniasis (ATL) is an infectious, non-contagious disease with an average of 1.3 million annual cases. Brazil shows the highest prevalence in number of reported cases in the Americas, where the specific agent is the species L. (V.) braziliensis. The diagnosis is commonly performed by an association of clinical, epidemiological and laboratorial tests. The immunological approaches which are most commonly used in the routine are the indirect immunofluorescence (IFI) and the enzyme-linked immunosorbent assay (ELISA), however, they may present limitations. Therefore, alternative methods have been studied, and one of them is flow cytometry (FC). Thus, the aim of this work is to evaluate, by flow cytometry, the role of the humoral response in the development of cutaneous lesions of patients with the active disease before treatment (BT), and in those healed after therapeutic intervention (PT). Sera samples were inactivated. For the FC assays, promastigote forms of L. (V.) braziliensis obtained by the reference strain were expanded in Schneider’s medium until they reached exponential phase. After washing protocols, the concentration of the parasites was adjusted and they were fixed with 1% paraphormaldehyde. For the IFI and ELISA assays, the total antigen of L. (V.) braziliensis were used. In FC and IFI assays, the IgG antibody was conjugated to a fluorophore and in ELISA, to an enzyme. Regarding IFI results, from the evaluated sera, 92.85% (13/14) were positive on BT group, 61.54% (8/13) were positive 1 year PT, 70% (7/10) 2 years PT and 50% (5/10) 5 years PT were positive. In ELISA, 92.8% (13/14) of BT patients were positive; and 53.8% (7/13) 1 year PT, 88.8% (8/9) 2 years PT and 100% (5/5) 5 years PT (5/5) remained positive for ATL. In FC, 86% (12/14) of BT patients were positive and 77% (10/13), 80% (8/10) and 70% (7/10) of the patients 1, 2 and 5 PT, respectively, were negative for the assay. Based on the ROC curves analysis to compare the performance of the 3 tests, it was observed that the area under the curve (AUC) of IFI (AUC=0.879; IC95%= 0.754-0.954) was lower that FC’s (AUC= 0.890; IC95%=0.767-0.961), meaning it had a lower performance. Comparing ELISA and FC, it was observed that the AUC of ELISA (AUC=0.808; IC95%= 0.652-0.915) has differed from the one observed for FC (AUC= 0.896; IC95%= 0.758-0.970), where again, FC has shown a superior performance. As for the results of the IgG isotypes’ (IgG1, IgG2 and IgG3) standardization, it was observed that the best dilution for IgG1 was 1:400; for IgG2, 1:100; and for IgG3, 1:200. On the analysis to verify IgG1 applicability, it was seen that BT 63.2% of the patients were positive, and 1PT, 17.7%; 2PT, 72.8% and 5PT, 12.55% of the patients were positive. Therefore, we believe that FC can be used as a diagnostic tool for ATL since it is positive in the presence of the disease, being superior to the two conventional methods used in the laboratory routine. Also, the use of the IgG1 isotype has the ability to contribute as a more sensitive and specific diagnostic method when compared to the classical ones.