Bacharelado em Ciências Biológicas (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/5
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APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
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Resultados da Pesquisa
Item Perfis genômicos do transposon Tn4401 de isolados de Proteus sp., Providencia sp. e Morganella sp(2022-07-03) Silva, Larissa Almeida da; Freitas, Nara Suzy Aguiar de; Santos, Dayane da Silva; http://lattes.cnpq.br/0456504242112576; http://lattes.cnpq.br/6891650997818766Antibiotic resistance is a growing global threat to public health, causing high mortality. Mutation, genetic drift, horizontal gene transfer via transposons, and natural selection are the basic mechanisms responsible for the spread of resistance genes among bacterial populations. The Proteeae tribe is composed of three genera: Proteus spp., Morganella spp. and Providencia spp., opportunistic pathogenic bacteria that are resistant to [beta]-lactams. The highly mobile transposon Tn4401 from Klebsiella pneumoniae and Escherichia coli is characterized by containing blaKPC genes encoding [beta]-lactamase, ensuring resistance to β-lactams. The objective was to analyze the evolutionary history of the genomes of the Proteeae tribe and its Tn4401 transposons, through patterns, variations and syntenies of genome sequences and their neighboring regions. Initially, the sequences of K. pneumoniae Tn4401 (Kp15, Kp512, Kp529-1) were used as a query in BLASTn/NCBI to search for strains of the Proteeae tribe. Then the selected GenBank files were used in Islandviewer 4.0 software for similarity and synteny analysis and the FASTAS sequences were incorporated into ClustalW/MEGA 11. After alignment, the ratio non-synonymous for synonymous substitutions was established. Different isoforms of Tn4401 were revealed, between them Tn4401a, Tn4401b and Tn4401d containing blaKPC. Several patterns of gene sitenia of transposon were detected, involving deletions, inversions, duplications and mobility of neighboring gene groups, sometimes similar, sometimes different. Purifying selection was an active force in the β-lactamases of the analyzed strains. We also detected a possible relationship between the transposon isoforms and neighboring islands of resistance to Mercury, Chromate and Sulfonamide. These findings emphasize the continuous action of different evolutionary processes on plasmids and on the Tn4401 element, as well as the possible potential for dissemination of resistance genes by the Proteeae tribe.Item Prospecção da atividade antimicrobiana de polissacarídeos extraídos das paredes celulares das leveduras Kluyveromyces marxianus, Kluyveromyces lactis e Saccharomyces cerevisiae(2021-02-26) Araújo, Isabelle Maria Feitosa de; Ferreira, Éder Galinari; http://lattes.cnpq.br/7670722046479986; http://lattes.cnpq.br/5044579569126838Polysaccharides from the most diverse sources, may have pharmacological activities such as, for example, antioxidant, immunomodulatory, anticoagulant and antimicrobial. Bearing in mind that this last activity has not yet been evaluated, according to our bibliographic survey, for the cell wall polysaccharides of the yeasts Kluyveromyces marxianus and Kluyveromyces lactis, the objective of this study was to verify the antimicrobial activity of polysaccharides extracted from the cell walls of the yeasts Kluyveromyces marxianus CCT7735 and Kluyveromyces lactis CBS2359 and compare them to a non-Kluyveromyes yeast like Saccharomyces cerevisiae CAT-1. The susceptibility tests were performed in Petri dishes (90 x 15 mm) using the Kirby-Bauer disc-diffusion method as recommended by the M 100 supplement of the 29th edition of the Clinical & Laboratory Standards Institute. Ten microliters of the polysaccharide solution (10 mg/mL) solubilized in distilled water and sterilized by filtration were deposited on sterile paper discs (6 mm). Polysaccharides were tested against Escherichia coli ATCC 25922 (positive control: 100/10 μg of piperacillin-tazobactam); Staphylococcus aureus ATCC 29213 (positive controls: 30 μg of cefoxitin and 10 units of penicillin); Pseudomonas aeruginosa ATCC 27853 (positive controls: 100/10 μg of piperacillin-tazobactam and 10 μg of gentamicin); Klebsiella pneumoniae ATCC 700603 (positive control: 10 μg gentamicin) and Enterococcus faecalis ATCC 29212 (positive controls: 30 μg tetracycline and 10 units of penicillin). None of the cell wall polysaccharide samples evaluated inhibited the growth of the pathogenic bacteria strains tested. Thus, at least in native molecules, polysaccharides did not show antimicrobial activity. We suggest that further studies be carried out with the samples evaluated, either for other pharmacological applications/activities or with the chemical modification or modification of the concentration of these polysaccharides, followed by a new test for antimicrobial activity, among others.