01.1 - Graduação (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2
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Resultados da Pesquisa
Item Abordagem metabonômica no estudo da toxicidade de plantas da família convolvulaceae contendo swainsonina e calisteginas(2019-12-13) Nascimento, Ana Lessa Oliveira do; Souza, Francisco de Assis Leite; http://lattes.cnpq.br/5304030174444186; http://lattes.cnpq.br/1434642356384009The Compulsory Supervised Internship (CSI) was held at the Parisian Institute of Science and Technology of Life and Environment (AgroParisTech). The institute, considered the most important in France on life science research and development, is divided into eight centers across the country, two of which located in Paris, Avenue du Maine Center and Claude Bernard Center, located in the 5th arrondissement of Paris. There are 11 Research Units composed of one or more laboratories and teams of teachers, technicians, doctoral students and master's students, these units can be mixed (those under the supervision of more than one entity) or their own (supervised by the institute itself). More precisely, the CSI was conducted at the Mixed Research Unit (UMR) Systemic Modeling Applied to Ruminants (MoSar), which is administered by AgroParisTech and the National Institute for Agronomic Research (INRA), which is under the Ministry of Research and Ministry of Agriculture. The aim of the internship was to start the formation in metabolomics, showing how the methodology can contribute to the study of plant poisoning, in this case to the group containing swainsonine and / or calystegines, that affect farm animals in Brazil, and to produce a literature review on the subject of the internship. Metabonomics is an important research tool that allows investigating the metabolic pathways affected in a toxicological process. When performed properly and periodically throughout an experiment and with pre-designed protocols, conceived from a biological problem, it is possible to follow the evolution of the intoxication process, identify useful markers for the establishment of pathogenesis and diagnosis, and in the case of global metabolic, that is, one can make discoveries that had not been raised in the initial biological questioning. This is possible thanks to the holistic characteristic of the technique, which makes it possible to visualize and quantify even the most subtle variations in metabolites concentrations. Plants containing swainsonine and calystegines are responsible for numerous cases of animal poisoning in Brazil, mainly goats in the Northeast due to factors related to extensive and semi-extensive breeding, these substances cause a severe neurological syndrome that can lead from decreased productivity to even death of animals, which can cause economic loss to producers, who often have animals as the main source of income, making it very important to clarify the mechanisms involved in establishing this disease in order to find solutions to the problem.Item Relatório de Estágio Supervisionado Obrigatório (ESO) realizado no Laboratório de Ecofisiologia e Comportamento Animal (LECA-UFRPE), Recife-PE(2019-01-25) Chaves, Hisadora Advincula da Silva; Mendonça, Fábio de Souza; http://lattes.cnpq.br/1976344298387988; http://lattes.cnpq.br/1849357038300502Due to the importance of the veterinarian in zebrafish (Danio rerio) vivarium, the Estágio Supervisionado Obrigatório (ESO) was carried out during the period from September 20 to December 07, 2018, in the Laboratório de Ecofisiologia e Comportamento Animal (LECA) of the Departamento de Morfologia e Fisiologia Animal (DMFA) of the Universidade Federal Rural de Pernambuco (UFRPE) in Dois Irmãos, Recife, Pernambuco, Brazil. The report was divided into Chapter I, describing the stage entity and the activities developed, depicting the training developed at the stage, and Chapter II, which represents the experiment performed. Zebrafish has become internationally recognized as an experimental model for evaluating acute and chronic toxicity tests caused by chemical products in scientific research in recent decades. Serjania glabrata Kunth is a plant present in the Caatinga biome widely distributed in the Semiarid region of Northeastern Brazil and has been reported as toxic to goats by producers. Thus, this study aimed to evaluate the toxic effects of the ethanolic extract of the leaves of Serjania glabrata Kunth on zebrafish embryos (Danio rerio). The leaves of S. glabrata Kunth were collected in the county of Salgado de São Félix-PB and, from these, the ethanolic extract diluted in 1% dimethylsulfoxide with a concentration of 5 mg/mL was obtained. The parameters evaluated were heart rate, mortality and teratogenic effects, such as column and tail deformation, pericardial and vitelline sac edema, and coagulation, at 24, 48, 72 and 96 hours post fertilization intervals under an optical microscope. The concentrations of 10, 100 and 1000 μg/L did not cause teratogenic effects, as well as mortality, but caused alterations in heart rate.