01.1 - Graduação (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2
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Resultados da Pesquisa
Item Microbiota bacteriana associada a pele de Scinax x-signatus (Spix, 1824) (Anura: Hylidae) em Pernambuco, Brasil(2020-02-03) Santos, Lara Valesca Mendonça da Costa; Santos, Alcina Gabriela Maria Medeiros da Fonsêca; Moura, Geraldo Jorge Barbosa de; http://lattes.cnpq.br/1348666346504103; http://lattes.cnpq.br/4938571253882757; http://lattes.cnpq.br/6095901652697979The presence of a symbiotic microflora in extremely sensitive animals like amphibians is crucial for the development of an immune system capable of fighting the main opportunistic infectious agents in the environment. Scinax x- signatus is a anuro which has a general and synanthropic behavior occurring in forests and urban areas, but there are no identification studies of bacterial microbiota associated with skin of this animal. The objective of this study was to identify the skin microbiota of Scinax x-signatus in two areas in the Botanical Garden Reef (08 º 04’ S, 34 º 59’ W) and compare the resident and transient microorganisms. Rinses were performed with sterile distilled water to remove the animals from transient microflora and swabs wiped on the skin to collect the resident microflora. Samples were predictively you identify in selective media, as Chromogenic Agar, Cetremide, EMB Agar and MacConkey agar For the analyzes, we used Fisher Test and Cluster Analysis to verify the similarity between the collection areas. 24 sample were isolated from bacterial microflora and predictive methods were taxa 5: Pseudomonas spp. (43%) occurring in most samples, Escherichia coli (22%), Klebsiella pneumoniae (18%), Enterococcus faecalis (12%) and Staphylococcus aureus (4%), with the lower frequency. The microbiota from the anthropized area showed less similarity between the resident and transient samples. Cluster Analysis indicated that the microbiota from the control area was more similar among transients and residents samples, possibly due to the low degree of spatial heterogeneity. Scinax x-signatus specimes presented skin microorganisms of medical interest that are similar to other species of frogs. S. x-signatus has great ecological importance to ecosystems, and studies to research antimicrobial potential on the skin and in the microbiota associated with S. x-signatus are very important for the production of pharmaceuticals.Item "Isso é um cururu?" Um aplicativo como proposta educativa para conservação dos anfíbios anuros do Parque Estadual de Dois Irmãos, Recife-PE(2018) Rocha, Eric Bernardino Gadelha; Santos, Ednilza Maranhão dos; http://lattes.cnpq.br/5812920432455297; http://lattes.cnpq.br/9341342233938949Anuran amphibians belong to a group of vertebrates of great relevance in an ecosystem, because they are bioindicators and biocontroladores, however, they are very sensitive to the environmental changes. In Brazil, this group is one of the most diverse in the Atlantic Forest, however, due to the constant threats that this biome has been suffering, some species are on the list of endangered animals. Initiatives such as the National Plan of Action for Endangered Species have been stimulating and supporting proposals and conservation actions in this biome for these species and their area of occurrence, as is the case of Dois Irmãos State Park - PEDI. This Conservation Unit is located in the metropolitan area of Recife / PE, and has 40 species of anuran amphibians, five of them with some threat of threat. The present work aimed to develop and validate an application entitled "This is a Cururu?" As a didactic tool for environmental education for young people and adults, as well as, offer pedagogical possibilities regarding its use. This instrument, besides being an object of use of anurofauna researchers in field activities, will be an important ally in environmental education activities within the PEDI. The application developed in the free software Android Studio, highlights the threatened species and has twelve attributes on the animal, they are: image of the species, scientific name, popular name, state and international threat level, endemism, animal size in relation to the hand human, geographic distribution in Brazil, type locality, habit, habitat, reproduction mode and advertisement song. The test of the tool was carried out on January 18, 2019 in Zoonoturno, PEDI activity, the evaluation carried out with 24 people, being 98% considered highly relevant, corresponding to the expected expectations for the usability of the app as a didactic tool, highlighting the (90%), which is the differential of the application as an accessible didactic tool with a free proposal developed for mobile phones. In this work three pedagogical proposals were created with the use of the application that can be carried out with park visitors, as well as students of higher, middle and fundamental education.Item Fungos associados à comunidade de anfíbios anuros no Parque Estadual de Dois Irmãos, Recife-PE(2018) Silva, Priscilla Santana; Santos, Ednilza Maranhão dos; Amorim, Fabiana Oliveira; http://lattes.cnpq.br/5177064974304577; http://lattes.cnpq.br/5812920432455297; http://lattes.cnpq.br/3267265100305064The Amphibia Class suffers various natural or induced threat factors, causing these species to decline in the face of habitat loss and emerging diseases by viruses, bacteria and helminths. There is also another factor that has been causing mass declines in amphibian species caused by chytridiomycosis caused by the fungus Batrachochytrium dendrobatidis. The objective of this work is to obtain a diagnosis of the methodologies used to identify pathogenic fungi such as Batrachochytrium dendrobatidis as well as to verify the occurrence of fungi associated with the anuran amphibian community in Dois Irmãos State Park, Recife, PE. The methodology consisted of data collection through bibliographical analyzes covering information on methods used to detect the fungus Batrachochytrium dendrobatidis (Bd), in research bases such as the Capes, Scielo and Scorpus platform in scientific articles published in the years 2007 to 2017 ; in addition to collecting bimonthly for ten consecutive days, in the day and night shifts, from October 2015 to April 2017 at Dois Irmãos State Park, a Wet Atlantic Forest Conservation Unit located in the metropolitan region of Recife (8 ° 7 '30 "S and 34 ° 52'30" W), with an area of 1,157.74 ha, of which 14 ha is located the Zoo of Recife. The biological material was collected through sterile swabs applied to the surface of the back, belly, fingers and interdigital membranes of anurans, and this biological material is conserved at low temperatures for future microbiological analyzes. Obtaining the following results as the categorization of 8 techniques of identification of chytridiomycosis through a bibliographic review in 92 scientific articles published in 29 scientific journals, it being possible to obtain 31 types of methods to work with amphibians to obtain the final product that is the presence of the fungus Batrachochytrium dendrobatidis and for the microbiological analyzes were collected biological samples of 32 individuals of anurans distributed in 10 species of anurans belonging to 4 families of anurans, of these samples it was possible to identify fungi belonging to the family Trichocomaceae with the species of Penicillium aurantiogriseum ( Dierckx), Penicillium fellutanum, Penicillium citrinum, Aspergillus sp. and Paecilomyces sp., in addition to the genus Tremella sp. Belonging to the Tremellaceae family. This work performs and provides conservationist knowledge to reduce anuran declines as well as to provide data with pharmacological potentials.Item “O incrível mundo dos anfíbios e répteis da Mata Atlântica” ações educativas no entorno de unidades de conservação(2018) Amaral, Jéssica Monique da Silva; Santos, Ednilza Maranhão dos; http://lattes.cnpq.br/5812920432455297; http://lattes.cnpq.br/1830373986976646With more than 70% of its original coverage area degraded, the Atlantic Forest still has a huge biodiversity and shelter about 730 species of amphibians and repitles, however, due to advances in urbanization and tenacity of popular beliefs which incites the population's loathing towards these animals, some species are endangered. Since the protected areas are an important tool for the preservation of species, the use of educational actions is fundamental to dismystify inherited beliefs and clarify the importance of these animals to the human population being the main resource in the management plan of an UC. In this way, the objective of this work was to promote and evaluate educational actions through travelling exhibitions on public schools around three Conservation Units, Parque Estadual de Dois Irmãos (PEDI), Estação Ecológica do Caetés (ESEC-Caetés) and Refúgio de Vida Silvestre do Sistema Gurjaú (RVS Gurjaú), aiming at valuing the importance of Atlantic Forest's reptiles and amphibians, stimulating the sensitization of the human community to forests and animals. The exhibitions were performed for three consecutive years aimed at students from 7th year of elementary education to 2nd year of high school. Each exhibition had a collection of banners and communicative boards, representative forests scenery, pictures of species, interactive booklets, a written questionnaire, taxidermized and preserved animals, also had live animals. A total of 20 schools and 2,141 students participated in the actions, aged between 9 and 21 years old. The students were participative in all activities, they demystified legends and beliefs through monitor's explanations, also reported the use of reptiles and amphibians in human nourishment, through photographs showed the most common species in the area where they live, some of them relate coexistence with these animals as well as feelings such as affection, fear and curiosity. After the explanations some students showed interest in helping to conserve these animals and the biome, which was reflected in positive answers of the questionnaires, where they answered that in order to help preserve the Biome they must not kill animals, take care of the forest and raise awareness about the importance of species on their community and stated they will teach friends and parents about it in speeches such as "I'll tell my mom the forest has to be preserved", "Me and my friends, from now on, we'll be the heroes of animals". The environmental education is an essential tool for demystification, awareness and appreciation of the environment, inducing in humans the will to conserve, being a buttress for ecological balance.