TCC - Bacharelado em Ciências Biológicas (Sede)
URI permanente para esta coleçãohttps://arandu.ufrpe.br/handle/123456789/412
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Resultados da Pesquisa
Item Efeitos da elicitação isolada e combinada de ácido salicílico e metil jasmonato nos parâmetros biométricos e bioquímicos em Lippia alba (Verbenaceae)(2024-09-27) Paula, Maria Eduarda Magalhães de; Sperandio, Marcus Vinícius Loss; http://lattes.cnpq.br/4157291425794314; http://lattes.cnpq.br/8710784594902570Lippia alba is an aromatic shrub from the Verbenaceae family, primarily associated with Brazil's Caatinga and Cerrado regions. It is used in traditional medicine for its pharmacological properties, those being attributed to the essential oil the plant produces as a defense mechanism. A tool that can be used to increase the productivity of bioactive compounds in both in vitro and in vivo development is elicitation. This study aims to analyze the effects of isolated and combined applications of the elicitors Methyl Jasmonate and Salicylic Acid on L. alba, using biochemical and biometric data as parameters one week after the elicitation. Cuttings of L. alba were maintained in hydroponic cultivation and later transferred to a greenhouse. The combined and isolated treatments received a concentration of 4 mM Salicylic Acid and 2 mM Methyl Jasmonate. An additional group was established, receiving an application of 1% ethanol. The experiment was harvested one week after the elicitation. Elicited treatments showed significant differences in essential oil yield, oxidative stress analysis, and enzymatic activity compared to the control. Growth parameters, along with carbohydrate and protein levels, kept similar patterns in all treatments. In vivo, combined Salicylic Acid and Methyl Jasmonate elicitation increased essential oil yield on L. alba without affecting biomass production, suggesting an efficient modulation of the plant metabolism. Therefore, elicitation at the tested concentrations demonstrated to be a viable tool to optimize the production of bioactive compounds in this species in a short elicitation period.Item Influência da adição de NPK no crescimento, respostas bioquímicas e estresse oxidativo em Pavonia humifusa A. St.-Hil. (Malvaceae) sob estresse hídrico(2024-09-27) Nóbrega, Marina Morais; Sperandio, Marcus Vinícius Loss; http://lattes.cnpq.br/4157291425794314; http://lattes.cnpq.br/6953276255530257Water stress can trigger a series of plant metabolic changes, affecting their biochemistry and morphology. The variability of rainfall cycles creates challenges to adaptation. However, nitrogen supplementation (NPK) can play a crucial role in this context, as these nutrients are fundamental for maintaining metabolism, especially under stress conditions. The present study evaluated the effects of nutritional supplementation on the biochemical and morphological responses of Pavonia humifusa plants under water stress. After 21 days of supplementation, water stress was induced for 29 days. The experiment included two NPK treatments: with and without NPK associated with two conditions of water availability: control and stress. Biometric parameters (aerial and root dry biomass), physiological parameters (relative water content and SPAD index), primary metabolism parameters (pigment and proline content), stress markers (malondialdehyde and hydrogen peroxide) and enzyme activities (superoxide dismutase, catalase and ascorbate peroxidase) were analyzed. ANOVA analyzed the data at 5% and the means were compared by the SNK test using the R software. The analyses carried out indicate that after nutritional application with NPK, P. humifusa showed a better growth rate, as well as a higher total chlorophyll content in vivo. The plants supplemented with NPK also showed better adaptation to water stress with a reduction in oxidative stress. In addition, with NPK supplementation, P. humifusa responded to be less susceptible to water stress by increasing the enzymatic activity of SOD and Proline, the main osmoregulatory amino acid in water stress conditions. The results obtained suggest that nutritional supplementation with NPK plays an important role in biochemical and physiological regulation against water stress in P. humifusa.Item Influência dos ciclos de rega na anatomia e metabolismo bioquímico foliar de Cenostigma pyramidale (Fabaceae)(2022-10-04) Alves, Jakelline Santos; Silva, Flávia Carolina Lins da; http://lattes.cnpq.br/7547725170997480; http://lattes.cnpq.br/8593632087425943The species Cenostigma pyramidale is especially important in the ecological aspect and little explored in the economic and environmental sphere, in view of its great potential, besides being used for timber and medicinal purposes is a pioneer species with high potential for the recovery of degraded areas. This work aims to evaluate the physiological and anatomical responses of the leaves of one of these species, Cenostigma pyramidale under irrigation cycles and submitted to water deficit. The plants were submitted to four experimental groups, during the growing period, counting ten individuals per treatment: Control - daily watering; RD - daily watering; R7 - watering 1x in 7 days; R14 - watering 1x in 14 days. After five months of growth in the greenhouse, the individuals were submitted to a period of water deficit, where there was complete suspension of water, and then underwent recovery, from which five individuals from each treatment were collected at the end of each period for biochemical analysis and leaf anatomy. To determine the periods, analysis of relative leaf water content (RWC) and soil moisture was performed. It was observed that plants from more severe treatments proved to be more resistant, in general, to maximum stress, anatomically and biochemically, investing in adaptations such as tannins, crystals and stomatal chambers, still showing expressive photosynthetic rates, indicating that pre-contact with short intervals of water shortage affects leaf storage responses during a prolonged water shortage. The most efficient use of resources was seen in the treatment that was subjected to irrigation every fourteen days (R14), which showed a high rate of total free amino acids, proteins, higher rate of photosynthetic pigments, presence of an abundant palisade parenchyma, while showing a tendency to decrease the volume of the mesophyll, among other adaptations. Thus, with this work it was possible to conclude that previous contact with lack of water positively affects the species Cenostigma pyramidale, generating more efficient responses and providing important anatomical and biochemical changes in its leaves.