Bacharelado em Ciências Biológicas (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/5
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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Resultados da Pesquisa
Item Indicadores higiênico-sanitários identificados em águas envasadas, comercializadas na Região Metropolitana de Recife (PE)(2021-12-07) Santana, Shirlayne Ferreira de; Pires, Edleide Maria Freitas; http://lattes.cnpq.br/0468713660747009; http://lattes.cnpq.br/2120632629049039For the past years, the rising consume og bottled water in Brazil is a fact that is being studied by researchers of different parts of the country. The possible reasons for the case is the fact that bottled water transmits protection to the health, studies confirm different contamination levels in this kind of water. To ensure the health of consumers, it is required a quality standard for the commercialization of this product. An example of this, ministries as the Health Minister (MS) and the National agency of Health sanitization (ANVISA) establish microbiological parameters for the bottled natural mineral water destined to human consumption. For many reasons, it is considered important the information regarding hygienic sanitary indexes presented in the bottled water. This research has the purpose to evaluate the microbiological quality of the bottled water commercialized in the Region of the great Recife (RMR) by means of microbiological analysis of the hygienic sanitary indexes. Pseudomonas aeruginosa and Coliforms. There were evaluated 9 different brands of bottled water in 20L package, coded as A, B, C, D, E, F, G, H and I. From each brand there collected 5 samples in the period between August of 2018 and June of 2019, totaling 45 samples. For the microorganisms detection, there were used validated methods by the American Public Health Association (APHA), by the American Waters Works Association (AWWA) and by the Water Environment Federation (WEF). The obtained results confirmed the presence of Pseudomonas in 64% of the samples and of Coliforms in 66% of the samples, what may led to conclude the Pseudomonas aeruginosa and Coliforms can occur in natural mineral water bottled in 20L package commercialized in the RMR; an expressive number of brands showed to be unsafe due to the presence of microorganisms indicators of the hygienic sanitary conditions; the contamination levels by the Pseudomonas aeruginosa and Coliforms, vary among different brands of bottled water and beside what was found, we can find in the RMR market, mineral water brands that are considered safe, regarding the microbiological aspects. Such results are considered concerning, since the search for bottled water is, mainly, for the apparently guaranteed safety.Item Fungos associados à comunidade de anfíbios anuros no Parque Estadual de Dois Irmãos, Recife-PE(2018) Silva, Priscilla Santana; Santos, Ednilza Maranhão dos; Amorim, Fabiana Oliveira; http://lattes.cnpq.br/5177064974304577; http://lattes.cnpq.br/5812920432455297; http://lattes.cnpq.br/3267265100305064The Amphibia Class suffers various natural or induced threat factors, causing these species to decline in the face of habitat loss and emerging diseases by viruses, bacteria and helminths. There is also another factor that has been causing mass declines in amphibian species caused by chytridiomycosis caused by the fungus Batrachochytrium dendrobatidis. The objective of this work is to obtain a diagnosis of the methodologies used to identify pathogenic fungi such as Batrachochytrium dendrobatidis as well as to verify the occurrence of fungi associated with the anuran amphibian community in Dois Irmãos State Park, Recife, PE. The methodology consisted of data collection through bibliographical analyzes covering information on methods used to detect the fungus Batrachochytrium dendrobatidis (Bd), in research bases such as the Capes, Scielo and Scorpus platform in scientific articles published in the years 2007 to 2017 ; in addition to collecting bimonthly for ten consecutive days, in the day and night shifts, from October 2015 to April 2017 at Dois Irmãos State Park, a Wet Atlantic Forest Conservation Unit located in the metropolitan region of Recife (8 ° 7 '30 "S and 34 ° 52'30" W), with an area of 1,157.74 ha, of which 14 ha is located the Zoo of Recife. The biological material was collected through sterile swabs applied to the surface of the back, belly, fingers and interdigital membranes of anurans, and this biological material is conserved at low temperatures for future microbiological analyzes. Obtaining the following results as the categorization of 8 techniques of identification of chytridiomycosis through a bibliographic review in 92 scientific articles published in 29 scientific journals, it being possible to obtain 31 types of methods to work with amphibians to obtain the final product that is the presence of the fungus Batrachochytrium dendrobatidis and for the microbiological analyzes were collected biological samples of 32 individuals of anurans distributed in 10 species of anurans belonging to 4 families of anurans, of these samples it was possible to identify fungi belonging to the family Trichocomaceae with the species of Penicillium aurantiogriseum ( Dierckx), Penicillium fellutanum, Penicillium citrinum, Aspergillus sp. and Paecilomyces sp., in addition to the genus Tremella sp. Belonging to the Tremellaceae family. This work performs and provides conservationist knowledge to reduce anuran declines as well as to provide data with pharmacological potentials.Item Potencial imunomodulador da lectina de Canavalia brasiliensis em modelos de infecção causadas por Listeria monocytogenes(2018-08-23) Silva, Rayza Mirella Alves da; Lima Filho, José Vitor Moreira; Tavares, Lethicia Souza; http://lattes.cnpq.br/4128808335995892; http://lattes.cnpq.br/9476972124107533; http://lattes.cnpq.br/2032024789360400Listeriosis is an infectious disease transmitted by food contaminated with the bacterium Listeria monocytogenes, which can cause miscarriages and miningoencephalitis in animals and humans. Antibiotics are not always effective in controlling the disease, so the use of immunotherapeutics becomes an interesting strategy to combat the disease. Conbr lectin, for example, extracted from the seeds of the Canavalia brasiliensis (Brave-Fava beans) has immunomodulatory, antimicrobial and cicatrizant activities already reported in the literature. The objective of this study was to evaluate the potential of the ConBr lectin as an immunotherapeutic in the control of L. monocytogenes infection. For this, in vitro tests with culture of macrophages treated with the lectin and infected by L. monocytogenes were carried out in order to evaluate: lectin cytotoxicity (ConBr); quantify intracellular bacteria and survival of macrophages through infection. In vivo assays were also performed to investigate the influence of ConBr lectin on the clearance of L. monocytogenes from the liver and spleen and quantification of leukocytes present in the blood of experimental animals. The results showed that the lectin produced an antiinflammatory stimulus in the animals that was negative for the elimination of the L. monocytogenes bacterium, as well as reduced the total leukocyte count when compared to the control group. It was concluded that, although ConBr lectin did not contribute to the control of L. monocytogenes infection, there is potential for the control of inflammatory processes derived from systemic infections.