Bacharelado em Ciências Biológicas (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/5
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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Resultados da Pesquisa
Item PPARs no câncer de fígado: sintenia e interações aplicados a medicina evolutiva(2024-02-26) Leôncio, Thays Maria; Freitas, Nara Suzy Aguiar de; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/6891650997818766; http://lattes.cnpq.br/3551659636270655Liver cancer is the sixth most common diagnosis and the third cause of death. Peroxisome proliferator-activated nuclear receptors (PPARs) are a group of genes that act as transcription factors and have recently been indicated as potential therapeutics for liver cancer. Thus, we studied the PPARs genes associated with their interactions and synteny in an evolutionary medicine approach to liver cancer. The selection of the PPAR family and the primary analysis of their functions were obtained on the NCBI and UniProt platforms. The search for gene interactions was carried out using the protein association network in the STRING database, for the PPAR, PPAR and PPAR subtypes and the synteny analysis of this gene group was carried out. The return of information indicated that the set of genes and gene products evaluated have multiple functionalities of varying magnitude and complexity, being related to liver metabolism and its risk factors or not with liver cancer. In addition to being Mendelian units, they also mainly present positive and negative pleiotropic effects, directly or indirectly involved with liver diseases or healthy phenotypes, such as activities related to thyroid function, spermatogenesis, bone formation and differentiation, satiety control, circadian cycle, among others. These analyzes that are just beginning show promise for an early prognosis involving not only the molecular interactions of PPARs, but their positions in the genome. In this sense, genes are also modulated sometimes to develop their tumorigenic expression, sometimes not, suggesting that their study focused mainly on a healthy phenotype can facilitate preventive actions. This basic approach to evolutionary medicine identified new biomarker targets for hepatocellular carcinoma that could be effectively useful for future genetic evaluations, aiming to better prevent diseases and promote health.Item Diversidade de genes de resistência em bactérias de ambientes extremos(2022-10-07) Silva, Erivelton Gomes da; Freitas, Nara Suzy Aguiar de; http://lattes.cnpq.br/6891650997818766; http://lattes.cnpq.br/9369370749452563Bacteria from extreme environments are poorly understood and the evolutionary histories linked to resistance and virulence gene patterns are still hidden. Although they are usually associated with a single extreme condition, they are often described as multi-resistances, which we assume is due to their rich genetic arsenal. Studying the diversity of these genes can help us to understand how bacterial life adapts in the scenario of environmental changes resulting from human action. This work studied the diversity of resistance mechanisms in bacteria and their shared genes between representatives of the Terrabacteria and Proteobacteria taxa. 16 genomes from 12 genera was selected, including thermophilic, psychrophilic, halotolerant, radiotolerant, acidophilic and resistant to heavy metals bacteria, in addition to 44 resistance genes. A phylogenetic tree was constructed with the 16S rRNA sequences (MEGA software). The sequences of the genes of interest were aligned against the NCBI/BLAST database, and their relationships to Mobile Genetic Elements (MGEs) obtained (IslandViewer 4). Among the gene products, we highlight the Quorum Sensing molecules for biofilm formation, present among phylogenetically distant taxa, where homologous signalers and receptors can be used to understand multi-resistances in extreme environments. On the other hand, we also found genes that act together in the creation of resistance, such as the mutS/mutL DNA repair genes, or the resistance genes to several phaE/phaC stressors, but which in some taxa showed the absence of one of alleles, or significant variations in the percentage of alignment of the alignments, indicating a possible difference in functionality. Other genes were more restricted to certain taxa, such as the ddrD of the radiotolerant Deinococcus radiodurans, which acts within a specific scenario of radiation and nutritional scarcity, in which case the improvement of a single gene/product led to a multi-resistance mechanism. Another example of restrictiveness is the phaE gene of the multidrug-resistant Rubrobacter xylanophilus, which cooperates in robustness and resistance to stress in this species. We also observed three cases of correlation between MGEs and resistance genes: the first in the occurrence of the radiotolerance gene recA in Genomic Islands in Thermus sp; another in the relationship of MGEs and Genomic Islands with the ars and cad genes, for arsenic and cadmium resistance, respectively, in Geobacillus stearothermophilus; and finally, the relationship of the Acidiphilium sp gene kdpB with plasmids in several of the taxa studied. This evidence indicates that, at least for a small part of these mechanisms, there is a potential for sharing resistance genes through Horizontal Gene Transfer (HGT). This potential for mobility could be an excellent biotechnological tool in the genomic editing of bacteria used in the bioremediation of contaminated environments. We believe that further studies of patterns and variations, phylogenetic analyzes and correlation of these genes with MGEs and genomic islands, may be ways to understand more about the diversity of resistance genes in extremophile bacteria.Item Análise in silicode iniciadores de genes referência utilizados como normalizadores em estudos utilizando qPCR para avaliação da expressão de isolados de Klebsiella pneumoniae(2019) Fonseca, Bárbara Schneyder Oliveira Pereira da; Almeida, Anna Carolina Soares; Nascimento, Crisvânia Pedrosa dos Santos; http://lattes.cnpq.br/9308656350291661; http://lattes.cnpq.br/4891800920829895; http://lattes.cnpq.br/0924501124844316Klebsiella pneumoniaeis a pathogenic bacterium considered to be an "urgent threat to human health"becausethe number of antibiotic-resistant bactéria is increasing, especially those considered to be the last line for its treatment, such as colistin.Forthis it is necessary to understand their mechanisms of resistance to know the best forms of treatment and to develop new drugs to treat infections. For this purpose, real-time quantitative PCR in relative expression studies has become one of the most effective tools to understand bacterial functioning at the transcriptional level, but for the results to be reliable and real, it is necessary to perform the normalization step, which among the possible the most common is through the useof reference genes. However, the choice of the genes to be used as normalizers among the genes pointed out in the literature has been controversial and, in many cases, with little reliability. This difficulty would be eliminated if there were a robust database for various types of studies for species other than humans and rats. Thus, there was a need to evaluate amongthe bacterial gene expression studies using qPCR, as normalizing genes and the primers used to amplify them.In a literature review, available in Pubmed, the 16Sand rpoBgenes were most commonly used as normalizers. Through in silico analysis after literature analysis it was possible to observe that the sequences of shared primer pairs were only smaller series within the ideal standard and the best ones in the future usedin later experiments, among others only one referring to one of the genes most commonly used, the 16S.