Bacharelado em Ciências Biológicas (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/5
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APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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Resultados da Pesquisa
Item Produção de proteases por Aspergillus ochraceus URM 604 obtidas por fermentação em estado sólido utilizando farelo de trigo e resíduo de café como substrato(2021-03-03) Santos, Amanda Lucena dos; Porto, Ana Lúcia Figueiredo; Cardoso, Kethylen Barbara Barbosa; http://lattes.cnpq.br/4784303425329040; http://lattes.cnpq.br/4989617783837981; http://lattes.cnpq.br/3697084385877618Proteases are enzymes of great commercial interest, since it has several industrial applications, such as the pharmaceutical, food, beverages and cleaning products. Among the organisms capable of producing these enzymes are filamentous fungi, having as advantages the possibility of secreting enzymes in the extracellular medium and growing on low-cost substrates. The Solid State Fermentation (SSF) is one of the recommended techniques in the cultivation of filamentous fungi, especially because it simulates its natural habitat, favoring their growth. Aiming the importance of proteases and the growing demand of the global market needs, it is necessary to search for new sources and better production methods. Thus, the objective of this research was to produce proteases by the filamentous fungus Aspergillus ochraceus URM 604 under SSF using substrates derived from the agribusiness, in 2³ factorial design. It was investigated the influence of the type of substrate (coffee residue, wheat bran and 1: 1 coffee + wheat bran), amount of substrate (3g, 5g and 7g ) and humidity (20, 40 and 60%) to determine the ideal conditions for protease production. Fermentation took place for 7 days at 30 °C and the metabolic extract was used for further analysis. For biochemical and physical analysis the protein activity, total proteins, temperature and optimal pH of the obtained enzyme were determined. When analyzing the influence of the variables adopted in the 2³ factorial design, only the type of substrate was a significant parameter. The best substrate was wheat bran, which showed a specific enzymatic activity of 218.27 U / mg under 3g of substrate and 60% humidity. The other conditions also showed high results when compared to the literature. The optimum temperature of the enzyme produced was 50°C and the optimum pH was pH 8-9 (alkaline protease). Thus, this research shows that the fungus A. ochraceus URM 604 has biotechnological potential for protease production under SSF using low-cost substrates such as coffee grounds and wheat bran, this being the first report for the species.Item Remoção do corante Azo Direct Black 22 utilizando fungos Aspergillus(2021-12-06) Santos, Karolaine da Conceição Gama; Bezerra, Raquel Pedrosa; http://lattes.cnpq.br/1466206759539320; http://lattes.cnpq.br/8911087163041081During the activity of the textile industrial sector, characteristic effluents are generated due to their strong coloration and, in contrast to the benefits, concerns arise from the impacts caused by the presence of dyes in the effluents. As they are difficult to degrade and have high toxicity, dyes lead to the eutrophication process and a reduction in the photosynthetic rate in water bodies, in addition to presenting toxic bioaccumulative potential. Therefore, it is essential to treat wastewater prior to release into water bodies, with the bioremediation process that employs micro-organisms to degrade such compounds as an alternative. Thus, this study aimed to investigate the ability of fungi of the genus Aspergillus to remove the tetra-azo dye Direct Black 22 (DB22). A selection of fungi from DB22 dye discoloration (50 mg/ L) was performed using 1g of live biomass of A. japonicus (URM 5620), A. niger (URM 5741) and A. niger (URM 5838) with duration 2 hours of experimentation, under at room temperature and 120 RPM. The fungi that showed the best results were A. niger (URM 5741) and A. niger (URM 5838), which in the initial 10 minutes of the experiment removed the DB22 dye by 86% and 97%, respectively. Such fungi were used with values of 1 g and 3 g of live biomass to evaluate the influence of the amount of biomass, since 1 g of biomass presented at the end of the test better dye removal, reaching the maximum discoloration of 100% and 99% for A. niger (URM 5741) and A. niger (URM 5838), respectively. The decolorizing capacity between live and dead fungal biomass (1 g) was also investigated, and it was observed that the dead biomass had the best percentage of discoloration, 66% and 96% for A. niger (URM 5741) and A. niger (URM 5838), respectively, still in the first minute of rehearsal. In this way, showing the ability of Aspergillus to remove DB22. Therefore, having seen the efficiency of application of such a filamentous fungus, it is necessary to further investigate the fungal biological mechanism in the removal of the textile dye and evaluate different test conditions to later be applied in real effluent on an industrial scale in order to contribute to the reuse of water in the harsh region of the State.Item Fungos em água envasada em garrafões de 20 litros comercializadana região metropolitana de Recife-PE(2019) Nascimento, Irani Pinto do; Pires, Edleide Maria Freitas; http://lattes.cnpq.br/0468713660747009The quality of the water for consume is animportant factor for the health and life quality of the consumers.The Brazilian Health Minister (MS) guarantees the harmlessness of the water consumed by the Brazilians over the establishment of rules and standards based of the presence of toxic compounds and bacteria. The search for natural mineral water is great all over the world and many are the reasons that lead the consumers to acquire this product, among them the purity characteristics contribute for this rise. Although, studies show cases of contaminations by fungus in bottled mineral water, being found even in high counting, even in anoligotrophic environment. This can constitute health risk for the consumers, mainly by immunocompromised individuals. The Brazilian legislation does not include fungus in the microbiological analysis for potable water. This work shows the presence of fungus in bottled water of 20 liters, comercialized in the great are of the city of Recife (RMR).There were randomly selected fifty 20 L bottles of tendifferent brands. From each brand there were analysed 5 samples of different lots, accordingly methodology of plate counting established in the Standard Methods for the Examination of Water and Wastewater. Contaminants fungus were found in every brand in different concentrations, with the 10 brands here identified from A to J, 8 showed contamination by filamentous fungus and yeast (A, C, D, E, F, G, H, J) and a difference in the contaminant quantity.The B and I samples showed low contamination by filamentous fungus and did not showed the presence of yeast.The pH of the analysed samples variedfrom 4.1 and 5.4. The results of this research allowed to conclude that the bottled water in 20Lbottles comercialized in the RMR can be contaminated by fungus; that there is great variation in the contaminations by this kind of microorganisms and that the pH of the different brands analysed, between 4,1 and 5,4, can contribute to the presence and/or development of the contaminants fungus. It is expected that with these records, contribute for the inclusion of fungus in the potability parameters for the human consume. Because the confrontation of these information with from others researchers, shows that this kinds of water can be animportant path to the dissemination of fungus and diseases transmissions.Item Fungos associados à comunidade de anfíbios anuros no Parque Estadual de Dois Irmãos, Recife-PE(2018) Silva, Priscilla Santana; Santos, Ednilza Maranhão dos; Amorim, Fabiana Oliveira; http://lattes.cnpq.br/5177064974304577; http://lattes.cnpq.br/5812920432455297; http://lattes.cnpq.br/3267265100305064The Amphibia Class suffers various natural or induced threat factors, causing these species to decline in the face of habitat loss and emerging diseases by viruses, bacteria and helminths. There is also another factor that has been causing mass declines in amphibian species caused by chytridiomycosis caused by the fungus Batrachochytrium dendrobatidis. The objective of this work is to obtain a diagnosis of the methodologies used to identify pathogenic fungi such as Batrachochytrium dendrobatidis as well as to verify the occurrence of fungi associated with the anuran amphibian community in Dois Irmãos State Park, Recife, PE. The methodology consisted of data collection through bibliographical analyzes covering information on methods used to detect the fungus Batrachochytrium dendrobatidis (Bd), in research bases such as the Capes, Scielo and Scorpus platform in scientific articles published in the years 2007 to 2017 ; in addition to collecting bimonthly for ten consecutive days, in the day and night shifts, from October 2015 to April 2017 at Dois Irmãos State Park, a Wet Atlantic Forest Conservation Unit located in the metropolitan region of Recife (8 ° 7 '30 "S and 34 ° 52'30" W), with an area of 1,157.74 ha, of which 14 ha is located the Zoo of Recife. The biological material was collected through sterile swabs applied to the surface of the back, belly, fingers and interdigital membranes of anurans, and this biological material is conserved at low temperatures for future microbiological analyzes. Obtaining the following results as the categorization of 8 techniques of identification of chytridiomycosis through a bibliographic review in 92 scientific articles published in 29 scientific journals, it being possible to obtain 31 types of methods to work with amphibians to obtain the final product that is the presence of the fungus Batrachochytrium dendrobatidis and for the microbiological analyzes were collected biological samples of 32 individuals of anurans distributed in 10 species of anurans belonging to 4 families of anurans, of these samples it was possible to identify fungi belonging to the family Trichocomaceae with the species of Penicillium aurantiogriseum ( Dierckx), Penicillium fellutanum, Penicillium citrinum, Aspergillus sp. and Paecilomyces sp., in addition to the genus Tremella sp. Belonging to the Tremellaceae family. This work performs and provides conservationist knowledge to reduce anuran declines as well as to provide data with pharmacological potentials.