Bacharelado em Ciências Biológicas (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/5
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Item Levantamento e análise da variabilidade genética do gênero Siderastrea Blainville, 1830(2024-10-04) Lira, Jean Tácio Tôrres de; Amaral, Fernanda Maria Duarte do; http://lattes.cnpq.br/7026011892824176; http://lattes.cnpq.br/0569107903225207This study aimed to fill gaps in knowledge about the genetic and phylogenetic diversity of the genus Siderastrea (Blainville, 1830), important coral reef-building organisms, with a greater focus on the species Siderastrea stellata (Verrill, 1868). Partially bleached colonies of the species S. stellata were collected on Gaibu beach, from November 2022 to January 2023 and the genomic DNA of the colonies was extracted using the Wizard Genomic DNA Purification Kit. A specific primer pair was designed to confirm the species S. stellata and the identification of the colonies was performed by the conventional PCR technique. The analysis of the variability between the colonies was performed by the RAPD technique using 06 random ITS primers. Furthermore, to analyze whether there was genetic variability among the S. stellata samples in the Genbank genomic database, we downloaded all sequences and performed sequence alignment. Subsequently, phylogenetic analyses were performed among the deposited samples using the Mega platform. Finally, in silico restriction enzyme analyses were performed to search for restriction enzymes that could be used to differentiate between the isolates. The results obtained with the RAPD technique did not provide sufficient data to identify genetic variability among the samples analyzed in the present study. On the other hand, regarding the data from the phylogenetic analysis, it was possible to find differences between the sequences of isolates from different regions. There was greater genetic proximity between S. stellata isolates from Panama and Brazil and less proximity to the isolate from Mexico. Siderastrea savigneana stood out as the most distant ancestor within the genus. In addition, it was possible to detect preliminary genetic variations among the populations evaluated, using data from the in silico restriction analysis. Thus, this study provides a first approach to genetic analysis of variability within the species of S. stellata. However, more in-depth studies are needed and may help to clarify the complexity of the genetic variability of the genus, in addition to assisting in management and conservation strategies for these organisms and the marine ecosystems they support.Item Levantamento e caracterização dos macroinvertebrados bentônicos do naufrágio Pirapama (PE)(2022-10-07) Bezerra, Natanael Cicero Cavalcanti; Amaral, Fernanda Maria Duarte do; Santos, José Carlos Pacheco dos; http://lattes.cnpq.br/2185193566880590; http://lattes.cnpq.br/7026011892824176; http://lattes.cnpq.br/6395940424269126Coral reefs have great biodiversity, serving as a shelter, nursery and feeding place for several species of vertebrates and invertebrates. As with natural reefs, the consolidated artificial substrate character gives these structures the ability to recruit larvae from the most diverse marine organisms. For this reason, these artificial ecosystems have been described as facilitating the arrival of exotic species to the coast. Thus, the objective was to carry out a survey and characterization of the benthic macroinvertebrate community of the centenary Naufrágio Pirapama, seeking to classify it according to its occurrence status, geographic distribution and trophic groups, as well as to compare these data with the results published ten years ago in similar research on the same shipwreck, allowing a temporal evaluation of this community. The entire structure of the wreck was observed through scuba diving, and sessile and sedentary macroinvertebrates were recorded with the aid of underwater cameras, pencils and PVC plates. All individuals were photographed and/or filmed in as much detail as possible, with all data being recorded in situ for their classification and location in the wreck. Minimal collections were performed with the aid of a hammer, chisel, Zip Lock plastic bags, nylon clamps and conditioned in 70% or 95% alcohol. In the laboratory, all fauna was identified at the lowest possible taxonomic level, with the help of microscopes, specialists and pertinent bibliography, and all species characterization was done through specific academic literature. In total, 44 taxa distributed among the phyla Cnidaria, Echinodermata, Annelida, Porifera, Arthropoda, Chordata, Mollusca and Bryozoa were identified. Compared to the 2010 survey, of the 76 taxa, 31 of them were not registered, but, on the other hand, there were 12 new ones for the shipwreck. Currently, three trophic groups were observed (Suspensivores, Detritivores and Carnivores), while for the previous check-list, in addition to these, the Herbivore and Omnivore groups were recorded. For both periods, the Filtered suspensivores showed a predominance greater than 80%. Regarding its status, the evaluated community showed a predominance of cryptogenic and native species from 2010 to the present, with only three non-invasive exotic species: the octocoral Carijoa riisei, the red lobster Panulirus argus and the barnacle Balanus trigonus. All organisms currently observed in the Pirapama wreck are commonly reported from the Brazilian coast and Pernambuco coast, making the benthic macroinvertebrate community of the studied artificial reef very similar to those observed in coastal reef environments.