Licenciatura em Química (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/26
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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2 resultados
Resultados da Pesquisa
Item Caracterização eletroquímica das interaçôes da lectina de sementes de Cratylia mollis (Cramoll) com glicose e eritrócitos de coelho em biossensor de MOF de [Cu3(BTC)2.(H2O)3]n(2022-05-31) Carvalho, Maryana Hermínio de; Coelho, Luana Cassandra Breitenbach Barroso; Freitas, Kátia Cristina Silva de; http://lattes.cnpq.br/5859266863241551; http://lattes.cnpq.br/2944428818449047; http://lattes.cnpq.br/0134945103585503Biosensors are small devices known for their selectivity, high specificity and sensitivity with the respective analyte even at low concentrations. Thus, we aimed to evaluate the charge transfer and electrochemical potential of the plant popularly known as camaratu bean, a forage native to the Caatinga biome, rich in shrub legumes, from which Cramoll lectin is extracted from Cratylia mollis Mart. electrochemical biosensor using the crystalline polymer MOF- [Cu3(BTC)2.(H2O)3]n.. to immobilize and characterize the physical and chemical properties of C. mollis (Cramoll) seed lectin and its interaction with free carbohydrates (glu-cose) and carbohydrates on the surface of rabbit erythrocytes. Lectin immobilization in MOF was effective for the construction of the electrochemical system, showing itself to be sensi-tive to conformational changes, being able to detect changes in the electrochemical poten-tials of Cramoll promoted in a buffered medium (200 mM potassium phosphate buffer, pH 7.0) with different glucose concentrations, highlighting the concentrations of 10 mM (96 - 121 mV), 15 mM (110 - 126 mV) and 20 mM (107 - 142 mV) in the time of 5 to 30 min. In the cyclic voltammetry, the interaction in the potential range from -0.2 to 0.0 V stood out; with the current reaching 0.0003 A and at peak 2 in the potential range of 0.4 to 0.5 V; with the current reaching 0.0007 A. The interaction of the carbohydrate present on the surface of the rabbit erythrocyte was also monitored by the potentiometric method, detecting changes in the Cramoll-glucose potentials, even in the presence of rabbit erythrocytes. Through SEM, it was possible to observe Cramoll immobilized on the MOF surface, proving the specificity of the ligand (glucose-lectin) through the morphological changes of this protein in this process. This electrochemical model, Cramoll/MOF biosensor, is effective for evalu-ating free lectin/carbohydrate or in the erythrocyte membrane.Item Sistemas eletroquímicos para tratar amostras de água contendo corante têxtil como ferramenta didática para divulgação da poluição hídrica(2021-07-07) Neves, Eloisa Ferreira; Freitas, Kátia Cristina Silva de; http://lattes.cnpq.br/5859266863241551; http://lattes.cnpq.br/9764373408949634Didactic workshops for scientific and technological dissemination, in addition to contributing to motivational aspects for the development of learning, are an interactive strategy that can provide the development of science learning. This research aims to use electrochemical systems to characterize the physicochemical properties of the coagulant lectin from Moringa oleifera seeds (cMoL) in the treatment of water samples containing indigo-type textile dyes as teaching tools for scientific dissemination about water pollution in effluents from the textile industry. The methodological development of this research comprises the development of aluminum piles using aluminum cans and PET bottles for the treatment of indigo-type dye solution; the determination of electrochemical potentials and cyclic voltammograms generated by the agglutination of cMoL immobilized on the MOF with an aqueous solution of indigo carmine dye; the development of potentiometric biosensors for the determination of indigo dye residues in aqueous media. Considerable differences were observed in the electrochemical potentials in relation to the platinum electrode (Pt), MOF coated platinum working electrode (Pt/MOF), MOF coated platinum electrode and immobilized cMoL (Pt/MOF/cMoL) and when interacting Pt /MOF/cMoL with different concentrations of galactose. Cyclic voltammetry showed results with significant differences between the untreated dye sample and the dye samples after treatment. The development of didactic workshops in school spaces to validate the electrochemical system as a didactic tool applied to learning and dissemination about water pollution caused by the disposal of indigo dyes in textile industry effluents is relevant for the construction of concepts related to electrochemistry.