Bacharelado em Ciências Biológicas (UAST)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2931
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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2 resultados
Resultados da Pesquisa
Item Atividade antibacteriana do óleo essencial de Croton tetradenius Baill frente a espécies de bactérias patogênicas(2019) Silva, Maria Aliny Nathalia Almeida; Silva, Ana Luiza da; http://lattes.cnpq.br/4162831591004569; http://lattes.cnpq.br/1069558787287430Medicinal plants of northeastern Caatinga are widely used in folk medicine by local communities, due to the therapeutic potential they present. Bacterial resistance to multiple drugs has increased in recently due to the indiscriminate use of antibiotics, generating the need for new broad spectrum drugs. Essential oils are known to be complex mixtures of volatile, lipophilic and odorous organic compounds that have numerous constituents due to secondary metabolites. This work aimed to evaluate the antimicrobial activity of Croton tetradenius (Euphorbiaceae) essential oil collected in the municipality of Triunfo-PE, obtained from the leaves by the hydrodistillation process. The evaluation was made by the paper disc diffusion method, in Petri plates, containing the MüellerHinton Agar medium, sown with bacterial suspensions previously adjusted to a 0.5 of the Mac Farland scale of three isolates. Discs containing 10 L and 15 L of the undiluted essential oil were added to the plates and incubated at 36 °C for 24 hours. Paper discs with the antibiotic Amikacin 30 μg/disc were used as positive control. The tests were performed in triplicates and the inhibition halos established in the average of the halos measured for each species of bacteria. The results show that Croton tetradenius essential oil has antimicrobial activity against the tested isolates Staphylococus aureus and Escherichia coli, except for Klebsiella pneumoniae which was resistant to the tested essential oil.Item Fungos de solo do semiárido pernambucano como fonte de compostos com atividade antibacteriana(2019) Souza, Denise Aparecida dos Santos; Siqueira, Virgínia Medeiros de; http://lattes.cnpq.br/5524459744893735; http://lattes.cnpq.br/3722098868591576Microorganisms are the main agents of biochemical activities occurring in the soil, being important for the proper functioning of ecosystems, in addition, several natural compounds are produced by microorganisms belonging to the soil, especially fungi, which are an excellent source for exploration of new secondary metabolites with antimicrobial activity, for the production of antibiotics. Thus, the objective of this work was to isolate, quantify and evaluate the antimicrobial potential of filamentous fungi isolated from soil in a preserved area of the Pernambucano semi - arid region, collected at the State Conservation Park Mata da Pimenteira, in the municipality of Serra Talhada - PE. For this, the soil samples were collected in two periods (drought and rain), from which the isolation and quantification of fungi in two culture media (Dextrose Bata Agar and Sabouraud Agar - SAB) by the serial dilution technique. This step was performed with three replicates in each period. Subsequently, the test of antimicrobial activity of fungi against bacteria was also carried out using the gelose block methodology. As a result, a higher quantification of fungi was obtained in the dry season, with higher growth of CFUs in BDA culture medium with 8.6 x 106 CFU / g, followed by 8.3 x 104 CFU / g in SAB. Thirty-one colonies were selected from the collection in drought, of which nine were identified to date as Aspergillus, Penicillium or Eurotium and of which, 20 were submitted to the antimicrobial activity test. Isolate 05 showed a 32 mm diameter inhibition halo against Staphylococcus aureus. Five other fungal isolates were active against Escherichia coli and five against Klebsiella; no fungus has shown activity against Salmonella. In relation to the rainy period, 11 isolates were identified, such as Aspergillus, Penicillium, Cladosporium and Paecilomyces. Of the isolates submitted to the antimicrobial activity test, none of the isolates showed inhibitory activity against the bacteria. From the obtained results, it was possible to verify the antimicrobial potential presented by the fungi in the period of drought, emphasizing its importance in search of new compounds of interest of the pharmaceutical industry.