Bacharelado em Ciências Biológicas (UAST)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2931
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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Resultados da Pesquisa
Item Análise de lacases de microrganismos com aplicações em biorremediação usando ferramentas de bioinformática(2022-10-21) Silva, Andrey Giordane Costa; Buarque, Diego de Souza; http://lattes.cnpq.br/7609652740088882; http://lattes.cnpq.br/8075252796586989The improper disposal and dumping of household waste, industrial waste, electronic waste, fertilizers, pesticides can elevate environmental concentrations of contaminants that cause significant impacts on human health and biodiversity. Given this problem, the development of technologies that assist in the environmental treatment of sites contaminated by these xenobiotics is of great importance. An applicable method for environmental remediation is biodegradation by enzymatic catalysis. Fungal lacases (in particular those of the genus Trametes) have a great potential for application in the area of wastewater treatment and bioremediation. Thus, a sequence analysis becomes important for the determination of lacases from some microorganisms. For this, we used the 1KYA, which represents the code of an active lacase structure from T. versicolor present in the Protein Data Bank (PDB). This structure is complexed to the ligand 2,5-xylidine, which is derived from commercially used solvents. Through this analysis, it is possible to understand structural factors important for the enzyme to detoxify environmentally harmful compounds, such as 2,5-xylidine. The structures and binding sites were analyzed using the BIOVIA Discovery Studio Visualizer 2021 program, where we were able to identify the amino acid residues and bonds that are part of the lacase 1KYA site that interact with 2,5-xylidine. To identify important structural factors in the sequences of lacases from microorganisms, a comparison was made in the primary sequence of the active lacase (1KYA) with a known sequence of the lacase from Trametes versicolor to determine what would be the degree of homology between them and if all amino acids that are part of the active site identified. By checking the degree of homology between different types of lacases from different organisms, it was possible to identify sequences of 16 microorganisms with a percentage equal to or greater than 79.56%. In addition, it was possible to identify the amino acid residues conserved in lacases from different organisms and the residues that changed among the sequences of this enzyme.Item Inferência filogenética de Apoidea (Hymenoptera) a partir da análise do grau de homologia do citocromo c por ferramentas de bioinformática(2022-06-01) Melo, Ericles Charles Da Silva; Buarque, Diego de Souza; http://lattes.cnpq.br/7609652740088882; http://lattes.cnpq.br/4571885030401284The Apoidea superfamily (Hymenoptera) is home to about 30,000 recorded species and consists of several subgroups of apoid bees and wasps, with different types of habits from kleptoparist to eusociality. The species of this subfamily can be identified by body division (head, mesosoma and metasoma) and by the presence or absence of hair on the body. Due to the great difficulty in establishing molecular markers that can infer the phylogeny of this group, we aimed in this work to verify if cytochrome c, a protein essential to oxidative metabolism and energy production, highly conserved in species, can satisfactorily fulfill this role. The work consisted of two approaches: 1) the search for the primary sequences of cytochrome c of organisms of the Apoidea superfamily available in biological databases (NCBI Protein), for subsequent multiple alignment and obtaining a phylogenetic tree through the MAFFT program; 2) comparison with the phylogenies present in the literature. FASTA sequences were obtained from 15 species, all containing 108 amino acid residues. The cladogram obtained from the proposed alignment shows that the Bombini and Apini tribes seem to form a sister group. The study also showed that the common ancestor that gives rise to the Euglossini tribe also gives rise to the Bombini, Apini and Meliponini tribes, thus demonstrating that Apini and Euglossini can be paraphyletic groups. Despite the few Apoidea cytochrome c sequences available in the NCBI Protein, it was possible to observe that the cladogram seems to go against some proposals in the literature, suggesting that cytochrome c seems to be promising for this purpose. However, this study does not immediately propose a new classification, as it would be necessary to analyze a greater number of primary sequences from different Apoidea species. Therefore, more studies are needed in different groups among the Hymenoptera so that it is possible to use cytochrome c as a possible marker.