TCC - Bacharelado em Ciências Biológicas (Sede)
URI permanente para esta coleçãohttps://arandu.ufrpe.br/handle/123456789/412
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Item Aspectos microbiológicos das especiarias comercializadas na Região Metropolitana do Recife/PE(2022-04-18) Guedes, Fernanda Gabriela dos Santos; Shinohara, Neide Kazue Sakugawa; http://lattes.cnpq.br/7105928729564845; http://lattes.cnpq.br/3714282848550009The first great globalization of the Modern Age, initiated by the Portuguese and Spanish in the 15th and 16th centuries, was strongly stimulated by the search for new sources of spices, hitherto unknown to the European nobility and which were quickly incorporated into their culinary repertoire. The practice of using spices adds flavor and naturally preserves food, due to the presence of essential oils with antimicrobial action. The objective was to investigate the presence/absence of possible pathogens in spices: Black pepper (Piper nigrum), Oregano (Origanum vulgare) and Urucum (Bixa Orellana L.), marketed in the Metropolitan Region of Recife (RMR). Microbiological tests were carried out to verify the hygienic-sanitary conditions of the spices in compliance with current legislation. All samples were absent from Salmonella sp., Stapylococcus aureus, Bacillus cereus and Escherichia coli. In the research of molds and yeasts, the highest concentrations were found in oregano, followed by black pepper and annatto. The high presence of fungi in spices is a public health problem, due to the ability of some species to produce mycotoxins, causing carcinogenic and hepatotoxic conditions. This health risk requires monitoring by public management throughout the production and storage chain, to mitigate the deleterious effects on the consumer population and on products from the food industry.Item Avaliação de métodos de manutenção de Bacillus thuringiensis var. israelensis(2018) Xavier, Ana Carolina Cavalcante; Porto, Ana Lúcia Figueiredo; Oliveira, José de Paula; http://lattes.cnpq.br/3540150611094753; http://lattes.cnpq.br/4989617783837981; http://lattes.cnpq.br/4483834181760838Due to the rising environment pollution and growth in the cases of health problems through the indiscriminate use of chemical pesticides, Bacillus thuringiensis based insecticides have been widely used as na alternative method to combat pests and vectors. Bacillus thuringiensis (also know as Bt ) is a bacterium that produces crystalline protein inclusions which are very toxic to many insect vectors and pests, and harmless to humans and animals. The proper maintenance of this microorganism is fundamental for the formulation success, therefore, the method must guarantee the highest yield of biomass and must also preserve the proteins produced by it.The most commonly used maintenance methods are with the Bt kept on the surface of paper disks, in frozen pellet, continuous filings with the lyophilized culture, and with the culture kept submerged in mineral oil. This work had as objective to determine the method that provides greater yield of bacterial biomass. Thus, three methods of maintaining Bacillus thuringiensis var. israelensis (cepa H-14/ IPA-CAV-Bti-0008): in paper disks, by freezing the pellet at -20 ºC and under mineral oil. Cultures were carried out in a bioreactor operating in batch discontinuous for each of the evaluated methods. Samples were taken every two hours to determine the growth curve of the microorganism by optical density (O.D). The analyzed parameters of pH, dissolved oxygen were automatically monitored by means of electrodes coupled to the equipment and inserted in the culture. The total yield of the bacteriaa biomass was determined by means of the flocculation / sedimentation process, with subsequent drying and determination of the dry mass. At the end of the cultures a polyacrylamide gel electrophoresis was performed to verify the profile of the proteins produced by the bacillus in each of the three methods. It was obtained that the method of preservation in mineral oil was the most efficient with regard to the higher yield of biomass, although the methods of maintenance in paper disc and the frozen pellet have the advantage of needing less storage space, risk contamination and cost.