TCC - Bacharelado em Ciências Biológicas (Sede)
URI permanente para esta coleçãohttps://arandu.ufrpe.br/handle/123456789/412
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Resultados da Pesquisa
Item Produção de enzimas utilizando resíduos de café como substrato em processos fermentativos: uma revisão(2021-12-07) Alves, Ywkelly de Lima; Bezerra, Raquel Pedrosa; Costa, Romero Marcos Pedrosa Brandão; http://lattes.cnpq.br/1797280118220965; http://lattes.cnpq.br/1466206759539320; http://lattes.cnpq.br/2310542251305353Microbial enzymes stand out due to their numerous biotechnological applications, and allied to this, the possibility of using residues as a substrate in the fermentation process can bring advantages in production on an industrial scale. Coffee residues, for example, have been shown to be a good means of obtaining a variety of enzymes. Thus, the objective of this work was to carry out a systematic review of the use of three different types of coffee residues for enzyme production, through fermentation processes, in order to identify the main classes of enzymes produced and the microorganisms used as fermenting agents, such as fungi, bacteria, cyanobacteria and microalgae. Thus, a bibliographic search was carried out in the databases since the last decade using the keywords “waste pulp coffee and enzyme” and, through inclusion and exclusion criteria, 26 articles were selected. About 30.76% of the works were produced in Indonesia and pointed to cellulase as the main enzyme produced. Solid state fermentation (SSF) was the most used process for the production of enzymes, representing 92.59%, and the fungi of the genus Aspergillus were most widely used in this process, with 23.07% of occurrence in the articles. Among coffee residues, pulp had the highest occurrence, appearing in 76.92% of the articles. Furthermore, fermentation time, residue volume, temperature and pH were essential parameters in the final result of obtaining the enzymes. Thus, it was observed that the coffee residue has potential as a substrate for obtaining different enzymes, mainly cellulosic ones, using fungi, especially those of the Aspergillus genus, in solid fermentation processes.Item Remoção do corante Azo Direct Black 22 utilizando fungos Aspergillus(2021-12-06) Santos, Karolaine da Conceição Gama; Bezerra, Raquel Pedrosa; http://lattes.cnpq.br/1466206759539320; http://lattes.cnpq.br/8911087163041081During the activity of the textile industrial sector, characteristic effluents are generated due to their strong coloration and, in contrast to the benefits, concerns arise from the impacts caused by the presence of dyes in the effluents. As they are difficult to degrade and have high toxicity, dyes lead to the eutrophication process and a reduction in the photosynthetic rate in water bodies, in addition to presenting toxic bioaccumulative potential. Therefore, it is essential to treat wastewater prior to release into water bodies, with the bioremediation process that employs micro-organisms to degrade such compounds as an alternative. Thus, this study aimed to investigate the ability of fungi of the genus Aspergillus to remove the tetra-azo dye Direct Black 22 (DB22). A selection of fungi from DB22 dye discoloration (50 mg/ L) was performed using 1g of live biomass of A. japonicus (URM 5620), A. niger (URM 5741) and A. niger (URM 5838) with duration 2 hours of experimentation, under at room temperature and 120 RPM. The fungi that showed the best results were A. niger (URM 5741) and A. niger (URM 5838), which in the initial 10 minutes of the experiment removed the DB22 dye by 86% and 97%, respectively. Such fungi were used with values of 1 g and 3 g of live biomass to evaluate the influence of the amount of biomass, since 1 g of biomass presented at the end of the test better dye removal, reaching the maximum discoloration of 100% and 99% for A. niger (URM 5741) and A. niger (URM 5838), respectively. The decolorizing capacity between live and dead fungal biomass (1 g) was also investigated, and it was observed that the dead biomass had the best percentage of discoloration, 66% and 96% for A. niger (URM 5741) and A. niger (URM 5838), respectively, still in the first minute of rehearsal. In this way, showing the ability of Aspergillus to remove DB22. Therefore, having seen the efficiency of application of such a filamentous fungus, it is necessary to further investigate the fungal biological mechanism in the removal of the textile dye and evaluate different test conditions to later be applied in real effluent on an industrial scale in order to contribute to the reuse of water in the harsh region of the State.Item Descoloração do corante têxtil marinho Direct 2R utilizando o fungo Aspergillus tamarii kita UCP 1279(2021-12-03) Cruz, Nayara Vitória dos Santos; Bezerra, Raquel Pedrosa; Silva, Raphael Luiz Andrade; http://lattes.cnpq.br/4770766127962026; http://lattes.cnpq.br/1466206759539320; http://lattes.cnpq.br/9306095300572849Synthetic dyes are widely used in textile industries, being characterized as easy- to-use substances, with great variety and profitability. Despite their attractiveness, dyes are also considered complex compounds, being characterized by their toxicity, hazardousness and difficult degradation. Treating wastewater before release into water bodies is essential for the environment. The biological method can be used with algae, bacteria, and fungi, the latter organisms being considered as one of the best models for treating effluents. Therefore, the present study sought to investigate the use of the fungus Aspergillus tamarii kita UCP 1279, isolated from the Caatinga Biome, to discolor a solution containing a textile dye from a laundry located in the interior of the state of Pernambuco. The discoloration of the Marine Direct 2R dye was evaluated at a concentration of 50 mg/L, these experiments were carried out using the microorganism A. tamarii kita under living and dead conditions, in different amounts of biomass (2, 4, and 6 grams). In addition, biomass reuse was evaluated, so that, after the first decolorization test of 120 minutes, two further decolorization sequences with 120 minutes each were conducted for all conditions. The best condition obtained with 2 grams of biomass was found in the dead condition, which, in just 15 minutes, discolored 97% of the color while, with 4 and 6 grams of biomass, the best performance was verified in the living condition, in which, at 15 minutes it reached 100% removals for both 4 and 6 grams of biomass. In the tests with the reuse of biomass, both conditions (live/dead) demonstrated efficiency in discoloring the dye in different quantities of biomass, at the end of the tests, thus showing the potential of the microorganism A. tamarii kita in carrying out the dye decolorization textile. Therefore, due to the effectiveness of the microorganism, the development of future research investigating the optimization of the process deserves to be studied, to provide an understanding of the best conditions for using A. tamarii kita, so that its use can be made viable on an industrial scale, as a new biological method to treat effluents containing textile dyes.