Navegando por Assunto "Antioxidantes"

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Análise da composição química do óleo essencial de espécies de Plectranthus cultivadas sob condições de estresse e avaliação Do seu potencial acaricida sobre o Tetranychus urticae
(2024-07-31) Silva, Beatriz Steffanie Gomes da; Moraes, Marcílio Martins de; http://lattes.cnpq.br/6957579091162269; http://lattes.cnpq.br/4696332206790980
Plants are living organisms capable of producing secondary metabolites as a defense and development mechanism. What stimulates the activation of secondary metabolism are changes in the natural conditions necessary for the survival of the plant, called biotic or abiotic stresses. Essential oils (EOs) are complex mixtures of constituents originating from the secondary metabolism of plants, and have different biological properties, from medicinal purposes to pest control. Thus, the objective of this study was to investigate the variation in the chemical composition of EOs from two species of the genus Plectranthus (P. barbatus and P. ornatus) under controlled stress conditions and the antioxidant and acaricidal potential against Tetranychus urticae (spotted spider mite). The species were separated into groups and subjected to different stress conditions: manual predation (PM), methyl salicylate (SM), methyl jasmonate (MJ), saline (S), water scarcity (E), ultraviolet radiation (UV), and the group without any stress (control). The EOs were obtained by hydrodistillation and the chemical composition was determined by gas chromatography coupled to mass spectrum (GC-MS). The data were subjected to multivariate analyses (PCA, heatmap and molecular network). The acaricidal activity was evaluated comparatively through the LC90 of the positive control Azamax and the antioxidant activity by the ABTS.+ and DPPH methods. For both species, the chemical composition identified in the control groups showed a predominance of constituents derived from sesquiterpenes, with (E)- caryophyllene as the major constituent. However, in the stressed groups, monoterpenes were present in higher percentages, significantly reducing the percentage of the major compound of the control. This variation was confirmed by all multivariate analyses used. The EOs of both species showed low acaricidal activity against the two-spotted spider mite, with mortality rates equal to 12.00% (P. barbatus) and 22.72% (P. ornatus). P. barbatus showed better antioxidant activity for ABTS.+ (62.74 μg/ml) while P. ornatus showed better for DPPH. (163.5 μg/ml). However, compared to the positive controls Trolox (4.13 μg/ml) and ascorbic acid (1.62 μg/ml), respectively, the activities presented by both species were relatively low. Therefore, it was possible to verify that the EOs of these species present antioxidant and acaricidal activities, although low, and that different stress conditions alter the chemical composition of the plant.
Avaliação da atividade antioxidante do extrato de beterraba (Beta vulgaris) na forma alcoólica e microencapsulada com maltodextrina
(2019-04-26) Melo, Monnykhe Lorena de Oliveira; Ribeiro, Daniele Silva; http://lattes.cnpq.br/1517959077516490; http://lattes.cnpq.br/4175557719549609
The beet has important health promoting effect, in its composition presents bioactive compounds as betalains and phenolic compounds, both recognized for having high antioxidant power. The betalains are nitrogenous and water-soluble dyes classified into betacyanins (red-violet color) and betaxantinas (yellow-orange color). These pigments are indicated as an alternative to bioactive natural dyes, however due to the instability to factors linked the food processing techniques are still not used on a large scale in the industry. Accordingly, a microencapsulation technique is shown as a possibility of increasing the presence of compounds in addition to facilitating handling during use. This study aimed to get the beet extract (Beta vulgaris) and evaluate betalains content, phenolics, flavonoids and antioxidant activity of the extract before and after microencapsulation spray (spray dryer), using as carriers maltodextrins of equivalent dextrose agents (DE) 15 and 20. The antioxidant activity was evaluated through the analysis of ABTS, DPPH and Cu2 + chelating ability, the betalains, phenolic and flavonoid contents were also evaluated. The beet alcohol extract gave better performance for all the antioxidant analyzes and theoretical bioactivities had no present of study, in comparison with the results obtained in the beet extract, in natura, and remained in the pH range of the product of the betalaínas, after the alcoholic extraction. These results demonstrated that the extraction of the bioactives present in the beet by the solid-liquid extraction process with 70% ethanol was efficient. Maltodextrins of equivalent dextrose of 15 and 20 were efficient for preservation of betalains and antioxidant activity. The microencapsulated powder with maltodextrin of 15 DE presented a higher yield in the atomization process compared to the powder microencapsulated with maltodextrin of 20 DE, presenting a better applicability for the microencapsulation of the alcoholic beet extract by spray drying. Both microencapsulated samples showed high retention of betalains and, therefore, the antioxidant activity and the dyeing power provided by the betalain pigments present in the extract were maintained, evidencing that the microencapsulation technique is a viable process for the protection of these pigments.
Avaliação da atividade antioxidante e oxidação lipídica em linguiça adicionada de cúrcuma (Curcuma longa L.) durante o armazenamento
(2024-03-04) Matos, Raísa Mayara Alves de; Costa, Ana Carolina dos Santos; http://lattes.cnpq.br/8927435119035218; http://lattes.cnpq.br/7735107065493452
Avaliação das atividades antioxidantes de exopolissacarídeos (EPS) produzidos a partir de Enterococcus sp. isolado de queijo coalho artesanal
(2022-05-26) Santos, Sybelle Montenegro dos; Soares, Maria Taciana Cavalcanti Vieira; http://lattes.cnpq.br/3917225553030089; http://lattes.cnpq.br/2605906179220160
Exopolysaccharides (EPS) are macromolecules produced by different microorganisms, including lactic acid bacteria (LAB), as probiotic strains of Enterococcus. These have, in addition to bioactive properties, such as antioxidants, which neutralize oxidative stress in the host and reduce the use of artificial products harmful to health, proven therapeutic efficacy and high availability, are also indicated for several biotechnological applications. In this scenario, trends in healthy food consumption open new perspectives for the application of biopolymers such as EPS produced by BAL as additives and functional ingredients. Therefore, the objective was to produce, extract and evaluate the antioxidant activities of EPS produced by Enterococcus sp. isolated from artisanal coalho cheese from the State of Pernambuco, by scavenging the radicals: 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic (ABTS) and superoxide, through tests carried out in the laboratory, using EPS at the following concentrations: 0.2 0.5 1 1.5 and 2 mg/mL. The results obtained were applied in mathematical equations, according to each method used, and compared with ascorbic acid as a positive control. In addition, the mean and standard deviation were used for the ANOVA test with a significance of p < 0.05. Among the results found, it is remarkable the antioxidant activities developed by EPS 133v through its ability to eliminate free radicals. The DPPH radical reached a minimum of 16% (0.2 mg/mL) and in the maximum fraction (2 mg/mL) 27% of scavenging capacity, ABTS and superoxide presented a minimum of 56% and 42% and the maximum of 72% and 47%, respectively, however, the hydroxyl radical showed scavenging capacity only at the maximum concentration (2 mg/mL). Therefore, it is noticeable that the EPS 133v of Enterococcus sp. has high exploration potential for the development of functional food ingredients or additives with economic and health benefits.
Avaliação dos efeitos da melatonina sobre a prole de ratas submetidas ao consumo crônico de álcool durante a gestação
(2023-09-12) Oliveira, Eduarda Gomes Monteiro de; Teixeira, Valéria Wanderley; http://lattes.cnpq.br/1716101030381501; http://lattes.cnpq.br/1716101030381501
De acordo com pesquisas do ano de 2021, o padrão de consumo de etanol da população brasileira é de aproximadamente 26,5%, destes, incluem se os bebedores abusivos e consumidores esporádicos. Os homens seguem sendo os maiores consumidores, porém, as mulheres são mais vulneráveis aos efeitos nocivos dessa substância. A exposição das células ao álcool pode gerar danos a diversos órgãos, desde o sistema nervoso, rins e principalmente ao fígado, sendo ele o principal órgão da biotransformação do etanol. A melatonina, um hormônio produzido e secretado principalmente pela glândula pineal, vem sendo utilizada como antioxidante e um potente anti-inflamatório no combate a danos causados por estresse oxidativo nos hepatócitos. Sendo assim, o objetivo do presente trabalho foi avaliar se a melatonina administrada durante a gestação pode prevenir os efeitos nocivos produzidos pelo álcool sobre o fígado da prole. Para isto, foram utilizadas 15 ratas albinas (Rattus norvegicus albinus), da linhagem Wistar, pesando aproximadamente 250 ± 30g. Provenientes do biotério do Departamento de Morfologia e Fisiologia Animal da Universidade Federal Rural de Pernambuco. Utilizou-se 12 filhotes de 60 dias de vida por grupo que foram eutanasiados para coleta do fígado (histologia e histoquímica) e realização de análises como: número, peso e comprimento dos filhotes. Formaram-se os seguintes grupos: Controle - filhotes de ratas que não receberam álcool; Álcool - filhotes de ratas que ingeriram álcool; Álcool + Mel – filhotes de ratas submetidas ao consumo de álcool e tratadas simultaneamente com melatonina. Foi administrado o álcool etílico nas ratas durante a prenhez via gavagem intragástrica na dosagem de 3g/Kg de álcool. A melatonina foi administrada em injeções diárias de 0,8 mg/Kg, sempre no horário das 18:00h às 19:00h, por via intraperitoneal. Os resultados obtidos mostraram não haver nenhuma má formação nos filhotes. Verificou-se redução do peso e comprimento dos filhotes nos animais do grupo Álcool, porém não houve diferença no número da prole. A análise histopatológica mostrou parênquima hepático com congestão das veias porta, centro lobular e os capilares sinusoides nos filhotes do grupo Álcool. Esses efeitos não foram evidenciados nos fígados dos animais dos grupos Controle e Álcool + Mel. A histoquímica para glicogênio revelou uma redução significativa do teor de glicogênio novamente apenas nos animais do grupo Álcool. Enquanto os animais dos grupos Controle e Álcool + Mel não manifestaram estas alterações. Na análise para colágeno foi evidenciado que nos animais do grupo Álcool, apresentaram um aumento na deposição de colágeno ao entorno das veias Centro lobular e porta, caracterizando um quadro de fibrose perivascular. Essas alterações não foram detectadas nos animais dos grupos Controle e Álcool + Mel. A análise morfométrica do fígado revelou uma diminuição significativa no percentual do índice organossomático nos animais do grupo álcool, além de redução do parênquima lobular e aumento do parênquima não lobular com isto, estes dados obtidos no presente trabalho, tem demonstrado que a melatonina foi capaz de prevenir lesões no fígado da prole de ratas submetidas ao consumo crônico de álcool durante a gestação e lactação, manteve o teor de glicogênio e colágeno semelhante aos animais do grupo controle, bem como o peso e comprimento dos filhotes.
Café arábica cv. Typica de Taquaritinga do Norte - PE: influência dos suportes de filtração na composição química e parâmetros cromáticos
(2023-04-14) Alcântara, Rafael Lopes de; Arruda, Luciana Leite de Andrade Lima; http://lattes.cnpq.br/6140791519234633; http://lattes.cnpq.br/7307624203390875
Composição química, atividade acaricida, propriedade antioxidante, inibição da acetilcolinesterase e toxicidade do óleo essencial das folhas de Sparattanthelium botocudorum
(2024-03-05) Silva, Mirian Luzinete da; Camara, Cláudio Augusto Gomes da; http://lattes.cnpq.br/5615678215435460; http://lattes.cnpq.br/4499498152298785
Sparattanthelium botocudorum is a species endemic to Brazil, commonly known as “Canela-brava”, this shrub is distributed on the coast of Paraíba and in the state of Pernambuco. Due to the scarcity of chemical and biological studies on this plant, the research aimed to determine the chemical composition of the essential oil of the leaves as well as the evaluation of the acaricidal, antioxidant and toxicity properties against Artemia salina. The essential oil, extracted from fresh leaves, through the hydrodistillation technique with the aid of a Clevenger-type device, had a yield of 0.40% m/m and its chemical characterization, through Gas Chromatography coupled to the Mass Spectrometer (CG-MS), emerged as the majority constituents were the sesquiterpenes Germanecrene D with (38.28 %), E-Nerolidol (18.95 %), [beta]-Caryophyllene (14.49 %) and Biciclogermacrene (14.18 %) and the single monoterpene Z-[beta]-Ocimene identified in the OE with a percentage of 0.37%. Residual contact bioassays were carried out, with the aim of identifying the effects of the oil on the two-spotted mite, and showed promising toxicity. The residual contact bioassay, in which the mites are arranged on already treated leaves, showed LC50 = 16.5 μL/mL. Furthermore, the oil also demonstrated ovicidal properties with an estimated LC50 of 1.20 μL/mL. And regarding repellency activity, the oil showed a better potential with the LC30 concentration 8.8 μL/mL. After evaluating the inhibition activity of the acetylcholinesterase enzyme, the essential oil from the leaves of S. botocudorum inhibited the enzyme, since the LC50 resulted was 2.9 μg/mL below the LC50 of the positive control, which is 5.95 μg/mL. mL. Furthermore, OE presented antioxidant properties for the free radical DPPH˙, estimating an EC50 = 110.3 μg/mL, while for the free radical ABTS˙+ it was EC50 = 49.23 μg/mL. Finally, the oil exhibited a toxicity concentration of 700 μg/mL for the toxicity test against A. salina, which compared to what described in the literature had no toxicity. Therefore, the study showed that the essential oil presented similar and promising effects compared to already commercialized acaricides, and that the bioassays presented different routes of action, therefore acting with different mechanisms on the pest and presenting a biological property of antioxidant activity.
Composição química, atividade citotóxica, acaricida e antioxidante do óleo essencial das flores de Caesalpinia echinata (Pau-brasil)
(2021-12-10) Rodrigues, Lucas Vitor Batista; Camara, Cláudio Augusto Gomes da; http://lattes.cnpq.br/5615678215435460; http://lattes.cnpq.br/7934872766687903
Belonging to the family Fabaceae, Caesalpinia echinata is a tree endemic to Brazil, popularly known as pau-brasil. It was widely explored because of the quality of its wood and the produc-tion of a red pigment and much coveted in colonial times for dyeing fabrics and due to predatory extractivism, it was almost extinct. Currently, wood is much sought after for the manufacture of bows for violins for its ideal stiffness and density. The scarcity of chemical and biological studies of different parts of the plant motivated the chemical composition of the essential oil of the flowers as well as the evaluation of the acaricide, cytotoxicity properties against saline ar-temia and antioxidant. The flowers of C. echinata were collected on the campus of the Federal Rural University of Pernambuco. The essential oil (EO) was extracted from the hydrodistilla-tion technique by a modified Clevenger type apparatus. The chemical composition of the oil was determined by gas chromatography coupled to a mass spectrometer. Residual contact was the method selected to evaluate the acaricide action on Tetranychus urticae and the biological model selected for cytotoxicity evaluation was That of A. salina. The EO of the flowers pre-sented monoterpenes as the main chemical class. The main compounds identified were linalool (32.33%), Z-jasmona (14.94%) and [beta]-E-ocimene (10.44%). The essential oil for the control of T. urticae presented a LC50 = 290.15 uL/mL. The EO revealed an antioxidant capacity for the free radical DPPH˙ estimated at EC50 = 364.10 ug/mL, while for the free radical ABTS˙+ was EC50 = 280.50 ug/mL. The oil exhibited a LC50 = 97.41ug/mL for the toxicity test against A. salina. This study showed that the essential oil of C. echinata flowers has acaricide, antioxidant and cytotoxic biological property against A. salina.
Efeitos da melatonina sobre o fígado e rins da prole de ratas submetidas ao consumo crônico de álcool durante a gestação e lactação
(2021-02-25) Santos, Yasmim Barbosa dos; Teixeira, Valéria Wanderley; http://lattes.cnpq.br/4292195468804301; http://lattes.cnpq.br/1783975917572458
Efeitos de dieta hipercalórica associada ou não a melatonina exógena durante a gestação e lactação sobre histofisiologia do baço na prole
(2019-11-29) Alpiovezza, Paloma Karen Bandeira de Melo; Teixeira, Valéria Wanderley; Lapa Neto, Clovis José Cavalcanti; http://lattes.cnpq.br/9465720906397764; http://lattes.cnpq.br/4292195468804301; http://lattes.cnpq.br/5763795995480674
Estudo morfométrico das placentas de ratas submetidas ao consumo crônico de álcool durante a gestação, tratadas ou não com melatonina exógena
(2021-02-25) Nascimento, Bruno José do; Teixeira, Álvaro Aguiar Coelho; http://lattes.cnpq.br/1539131079574469; http://lattes.cnpq.br/8213260513385508
Estudo químico e atividade sequestradora de radical livre da própolis de Trigona spinipes Smith (Arapuá)
(2022-06-10) Souza, Hélter José Silva de; Silva, Tania Maria Sarmento da; http://lattes.cnpq.br/9185334632055347
The stingless bee Trigona spinipes is popularly known as a primordial generalist species in the performance of ecosystem services, due to the speciesization of agri-cul-tural plants, as a dominant species in the interaction networks between plants and bees. Geopropolis, is a resin mixture collected from pro-resin resins, essential oils, pollen, wax, sugars and salivary secretions from bees. This work describes the chemical study of two samples of Trigona spinipes propolis collected in the Caatinga region of the Polo Petrolina-PE, in the areas that comprise the Vale de São Francis-co. The chemical study of the extracts and fractions was carried out by Ultra Perfor-mance Liquid Chromatography coupled with Diode Array detectors and Quadrupole Time-Time Quadruple Mass Spectrometer (UPLC-DAD-QTOF-MS/MS) and coupled Gas Chromatography a Mass Spectrometer (CG/MS). From the chemical study it was possible to observe the presence of substances from the classes of polyamines, fla-vonoids, terpenes, steroids and fatty acids. The two have similar chemical com-posi-tion. The biological study with total phenolics, flavonoids and the DPPH radical showed that propolis are rich sources of bioactive compounds with antioxidant po-tential. The pro-anti-inflammatory action presents protection against bacteria, virus, fungi and anti-inflammatory activities.
Impacto do estresse oxidativo na maturação de oócitos in vitro: efeito antioxidante do tanino
(2023-09-05) Ramires, Millena Mary da Silva; Souza, Andreia Fernandes de; Batista, André Mariano; http://lattes.cnpq.br/5615914349535394; http://lattes.cnpq.br/6354486109796073; http://lattes.cnpq.br/3731466705214441
In vitro conditions can end up promoting damage to oocytes due to oxidative stress, compromising their quality and success rates in embryo production. Research has reported that supplementing the in vitro maturation medium with different antioxidants helped mitigate damage from oxidative stress. Thes, tannin, which has antioxidant action, arouses interest in studies on its antioxidant potential in oocyte maturation. This study was carried out to promote greater scientific knowledge about the action of tannin on oocyte maturation. Ovaries from adult cows obtained from a commercial slaughterhouse were used. These antral follicles were aspirated to select oocytes with homogeneous cytoplasm and one or more compact layers of cumulus cells. After maturation, these cells were removed by pipetting for subsequent preparation of slides and reading regarding the cellular meiotic stage. The experimental groups consist of different concentrations of Tannin (Sigma), being: TA0 = no addition of Tannin (control); TA1 = 1 μg/mL Tannin; TA10 = μg/mL of Tannin and TA100 = 100 μg/mL of Tannin. With the experiment, similar maturation rates were obtained for all treatments tested: TA0 (79.31%) TA1 (72.63%) TA10 (80.95%) TA100 (73.11%). Regarding the cleavage rates obtained after fertilization, it was observed that treatments TA0 (73.13%) TA1 (63.38%) TA10 (70.90%) presented similar results, while treatment T100 (47.91 %) showed a deleterious effect with a significant decrease in cleavage rates. Therefore, it is recommended that tannin supplementation be carried out in amounts below 100 μg/mL.
Investigação dos efeitos anti-UV e antioxidante de extratos de cianobactérias da coleção CCAPE (Coleção de Cultura de Cianobactérias e Algas de Pernambuco)
(2023-04-20) Nascimento, Luana Cláudia Barros; Gama Júnior, Watson Arantes; http://lattes.cnpq.br/8692563615933473; http://lattes.cnpq.br/5227051951077900
As cianobactérias são organismos cosmopolitas, e dentre as propriedades que garantem a sobrevivência delas está a produção de fotoprotetores, sendo o aminoácido do tipo micosporinas (MAAs) e citoneminas os mais comuns. A citonemina é um pigmento hidrossolúvel encontrado na bainha extracelular de polissacarídeos, crostas ou colônias de cianobactérias e os MAAs são um grupo de mais de 20 moléculas hidrofílicas, podem ser encontrados no citoplasma e na bainha externa das cianobactérias. Ambos fotoprotetores têm um grande potencial em aplicações nas indústrias cosméticas, devido às capacidades fotoprotetora e antioxidantes, podendo ser um produto de cuidados da pele, como protetores solares e produtos antienvelhecimento. Com isso, o objetivo do trabalho é conhecer e investigar o potencial das cianobactérias terrestres isoladas da Mata Atlântica em produzir substâncias fotoprotetores (anti-UV) e suas atividades antioxidantes. Foram identificadas, fotografadas e descritas seis espécies de cianobactérias Komarekiella sp., Nostoc sp.1, Nostoc sp.2, Nostoc interbryum, Chroococcidiopsis thermalis e Plectolynbya sp. que são pertencentes às ordens Nostocales, Chroococcidiopsidales e Synechococcales. As cepas foram expostas à radiação UVA e luz branca pelo período de 24 horas. A biomassa foi liofilizada e seguiu para extração em acetona 100% (clorofila, carotenoides e citonemina) e metanol 20% (aminoácidos do tipo micosporinas), todos os extratos foram analisados por espectofotometria de luz. Os extratos metanólicos foram testados quanto à atividade antioxidante por ABTS. Na análise de fotoprotetores, notou-se que a espécie Nostoc sp.1 (CCAPE 85) teve uma produção signifitiva de MAAs em todos os comprimentos de onda após exposição UVA. Na análise antioxidante, o extrato metanólico das espécies (CCAPE-72), Plectolynbya sp. (CCAPE-76), Nostoc sp.1 (CCAPE-85) e Nostoc interbryum (CCAPE-77) tiveram uma atividade antioxidante significativa apósexposição à radiação UVA. As espécies Komarekiella sp. (CCAPE-72), Chroococcidiopsis thermalis(CCAPE-73), Plectolynbya sp. (CCAPE-76), Nostoc interbryum (CCAPE-77), Nostoc sp.1 (CCAPE-85) e Nostoc sp.2 (CCAPE-89) tiveram uma maior atividade antioxidante acima de 30% em leituraapós 20 minutos da reação com ABTS. A espécie Nostoc sp.1 (CCAPE-85) teve a maior produção signifitiva de MAAs em todos os comprimentos de ondas, como também de atividade antioxidante em exposição UVA e, por isso, pode ser uma alternativa promissora na indústria cosmética, visto que produz fotoprotetores de interesse (MAAs) e apresenta relevante atividade antioxidante. Os resultados contribuem para uma melhor compreensão da taxonomia dessas cianobactérias da CCAPE, com a caracterização morfológica das cepas e identificação em nível de gênero e/ou espécie. Os dados de antioxidante e fotoprotetores demonstram o potencial das cianobactérias de ambientes terrestres para produção desses compostos e a importância de expô-las à radiação UVA para ativar ou aumentar a produção dessas substâncias.
Otimização e caracterização físico-química de café produzido pelo método de extração a frio (Cold Brew)
(2021-12-07) Lopes, Camila Cristina da Silva; Arruda, Luciana Leite de Andrade Lima; http://lattes.cnpq.br/6140791519234633; http://lattes.cnpq.br/6822570935662148
Síntese e docking molecular de 1,2,4-oxadiazol como potencial agente antioxidante
(2024-07-30) Silva, Ryan Henrique Gomes da; Freitas Filho, João Rufino de; http://lattes.cnpq.br/9252404584350850; http://lattes.cnpq.br/6976073462477884
The present work aimed to synthesize and characterize 1,2,4-oxadiazoles 29a-c, and to evaluate their antioxidant capacity using in vitro and computational methods. For this purpose, the synthesis of an important precursor, the arylamide oximes 27a-c, was carried out using the magnetic stirring method, obtaining excellent yields (84.9% - 95.7%). Then, the synthesis of 1,2,4-oxadiazoles 29a-c was described using the solvent-free method, which does not use solvents, bases or salts, obtaining low to moderate yields (30.6% - 56.2%). Furthermore, these heterocyclics were characterized by IR, 1H NMR and melting point. Thus, with the application of the DPPH• and ABTS•+ free radical scavenging test, it was found that the 1,2,4-oxadiazoles presented better results in reducing these radicals in the ABTS•+ method than in DPPH•, with emphasis on compound 29b (EC50 = 4.055 μg.mL-1), which presented promising free radical scavenging capabilities very similar to the standard antioxidant TROLOX (EC50 = 4.1 μg.mol-1), used as a positive control. Regarding the computational procedures, the molecular docking method was used to verify the energetic stability and the types of interaction of the complexes formed with compounds 29a-c and a pro-oxidant enzyme, Xanthine Oxidoreductase (ID PDB: 1N5X), which is involved in the superoxide release process. Therefore, based on the LGA calculations of the AutoDock 4.2.6 program, it was observed that the 1,2,4-oxadiazoles used as ligands formed stable complexes with the target enzyme, highlighting compound 29c (ΔH = –7.69 kcal.mol-1), which generated a complex with more stable energy close to the original inhibitor ligand, Tei-6720 (ΔH = –8.63 kcal.mol-1), indicating these compounds as potential antioxidants.
Uso de micronutrientes e acido salicílico como meio de reduzir os impactos do deficit hídrico na cana-de-açúcar
(2023-09-04) Batista, Jackelyne Gomes Pereira; Oliveira, Emídio Cantídio Almeida de; http://lattes.cnpq.br/1078501992095596; http://lattes.cnpq.br/1681172566920873