03.1 - Graduação (UAST)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2926
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Resultados da Pesquisa
Item Análise de lacases de microrganismos com aplicações em biorremediação usando ferramentas de bioinformática(2022-10-21) Silva, Andrey Giordane Costa; Buarque, Diego de Souza; http://lattes.cnpq.br/7609652740088882; http://lattes.cnpq.br/8075252796586989The improper disposal and dumping of household waste, industrial waste, electronic waste, fertilizers, pesticides can elevate environmental concentrations of contaminants that cause significant impacts on human health and biodiversity. Given this problem, the development of technologies that assist in the environmental treatment of sites contaminated by these xenobiotics is of great importance. An applicable method for environmental remediation is biodegradation by enzymatic catalysis. Fungal lacases (in particular those of the genus Trametes) have a great potential for application in the area of wastewater treatment and bioremediation. Thus, a sequence analysis becomes important for the determination of lacases from some microorganisms. For this, we used the 1KYA, which represents the code of an active lacase structure from T. versicolor present in the Protein Data Bank (PDB). This structure is complexed to the ligand 2,5-xylidine, which is derived from commercially used solvents. Through this analysis, it is possible to understand structural factors important for the enzyme to detoxify environmentally harmful compounds, such as 2,5-xylidine. The structures and binding sites were analyzed using the BIOVIA Discovery Studio Visualizer 2021 program, where we were able to identify the amino acid residues and bonds that are part of the lacase 1KYA site that interact with 2,5-xylidine. To identify important structural factors in the sequences of lacases from microorganisms, a comparison was made in the primary sequence of the active lacase (1KYA) with a known sequence of the lacase from Trametes versicolor to determine what would be the degree of homology between them and if all amino acids that are part of the active site identified. By checking the degree of homology between different types of lacases from different organisms, it was possible to identify sequences of 16 microorganisms with a percentage equal to or greater than 79.56%. In addition, it was possible to identify the amino acid residues conserved in lacases from different organisms and the residues that changed among the sequences of this enzyme.Item Análise estrutural e uso de lacase de camarão para a degradação de antraceno(2022-10-19) Silva, Larissa Celestino da; Buarque, Diego de Souza; http://lattes.cnpq.br/7609652740088882; http://lattes.cnpq.br/5589996843765879Through the large population growth and technological development, the use of natural resources has become increasingly present and constant, also causing concerns, measures and solutions related to the environment. In this context, the oil spill has affected aquatic ecosystems. Furthermore, petroleum comprises a series of polycyclic aromatic hydrocarbons, which are not very volatile and are deposited at the bottom of aquatic environments. Therefore, the use of laccase enzymes can be used to combat petroleum hydrocarbons. The structure of the L. vannamei laccase showed conserved domains characteristic of this enzyme, such as those that are important for copper centers, since they are responsible for carrying out the oxidation of aromatic compounds. In addition, the sequence of this enzyme showed a higher percentage of identity when compared to the laccases of other organisms, this fact can be characterized due to the number of identical positions existing between their amino acid sequences. The present work addressed the use of bioinformatics tools to understand the structure of the laccase and, consequently, test the activity of the enzyme in the degradation of the anthracene hydrocarbon.Item Microalgas do Semiárido: florações nocivas, variabilidade sazonal e suas possíveis aplicações biotecnológicas(2018) Oliveira, Carlos Yure Barbosa de; Dantas, Danielli Matias de Macêdo; Olivera Gálvez, Alfredo; http://lattes.cnpq.br/7002327312102794; http://lattes.cnpq.br/3422902414863662; http://lattes.cnpq.br/3825860944561089Microalgae and cyanobacteria are eukaryotes and prokaryotes, respectively, photosynthetic and use atmospheric CO2, light and inorganic compounds for their growth. In addition, they produce nutritional, bioactive compounds and some species mayproduce toxins (like some species of cyanobacteria and dinoflagellates). The present study aimed to identify and isolate species from the phytoplankton community of reservoirs located in the semiarid Pernambucano and later to evaluate the biotechnological potential of microorganisms previously isolated from the center of cultureof the Laboratório de Produção de Alimento Vivo (LAPVI-UFRPE/Sede). For the collection of these organisms a network with a mesh of 20μm was used, being part of the collected material fixed in formaldehyde to 4% and the rest submitted to the culture. After the sorting of all collected material, the canonical correspondence analysis was performed to evaluate possible interactions between the abiotic parameters and the species found. The evaluation of biotechnological potential occurred by the bioremediation capacity test of Chlorella vulgarisin effluent from a biofloc system (BFT). Twenty-one taxa were recorded, most of them of the class Chlorophyceae. Among the species found, we highlight the presence of Ceratium furcoides, an exotic dinoflagellate that is found in a growing geographic expansion in Brazil. In the evaluation of bioremediation potential, the presence of Chlorella vulgaris promoted a reduction in the levels of nitrogenous and phosphate compounds of the BFT effluent of approximately, 79.2% and 48%, respectively. In addition to the removal capacity of these compounds, BFT effluent presented as potential medium for the cultivation of this microalgae.Item Microalgas do semiárido: florações nocivas, variabilidade sazonal e suas possíveis aplicações biotecnológicas(2018-09-23) Oliveira, Carlos Yure Barbosa de; Dantas, Danielli Matias de Macêdo; Olivera Gálvez, Alfredo; http://lattes.cnpq.br/7002327312102794; http://lattes.cnpq.br/3422902414863662; http://lattes.cnpq.br/3825860944561089Microalgae and cyanobacteria are eukaryotes and prokaryotes, respectively, photosynthetic and use atmospheric CO2, light and inorganic compounds for their growth. In addition, they produce nutritional, bioactive compounds and some species may produce toxins (like some species of cyanobacteria and dinoflagellates). The present study aimed to identify and isolate species from the phytoplankton community of reservoirs located in the semiarid Pernambucano and later to evaluate the biotechnological potential of microorganisms previously isolated from the center of culture of the Laboratório de Produção de Alimento Vivo (LAPVI-UFRPE/Sede). For the collection of these organisms a network with a mesh of 20μm was used, being part of the collected material fixed in formaldehyde to 4% and the rest submitted to the culture. After the sorting of all collected material, the canonical correspondence analysis was performed to evaluate possible interactions between the abiotic parameters and the species found. The evaluation of biotechnological potential occurred by the bioremediation capacity test of Chlorella vulgaris in effluent from a biofloc system (BFT). Twenty-one taxa were recorded, most of them of the class Chlorophyceae. Among the species found, we highlight the presence of Ceratium furcoides, an exotic dinoflagellate that is found in a growing geographic expansion in Brazil. In the evaluation of bioremediation potential, the presence of Chlorella vulgaris promoted a reduction in the levels of nitrogenous and phosphate compounds of the BFT effluent of approximately, 79.2% and 48%, respectively. In addition to the removal capacity of these compounds, BFT effluent presented as potential medium for the cultivation of this microalgae.