Licenciatura em Química (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/26
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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2 resultados
Resultados da Pesquisa
Item Caracterização eletroquímica das interaçôes da lectina de sementes de Cratylia mollis (Cramoll) com glicose e eritrócitos de coelho em biossensor de MOF de [Cu3(BTC)2.(H2O)3]n(2022-05-31) Carvalho, Maryana Hermínio de; Coelho, Luana Cassandra Breitenbach Barroso; Freitas, Kátia Cristina Silva de; http://lattes.cnpq.br/5859266863241551; http://lattes.cnpq.br/2944428818449047; http://lattes.cnpq.br/0134945103585503Biosensors are small devices known for their selectivity, high specificity and sensitivity with the respective analyte even at low concentrations. Thus, we aimed to evaluate the charge transfer and electrochemical potential of the plant popularly known as camaratu bean, a forage native to the Caatinga biome, rich in shrub legumes, from which Cramoll lectin is extracted from Cratylia mollis Mart. electrochemical biosensor using the crystalline polymer MOF- [Cu3(BTC)2.(H2O)3]n.. to immobilize and characterize the physical and chemical properties of C. mollis (Cramoll) seed lectin and its interaction with free carbohydrates (glu-cose) and carbohydrates on the surface of rabbit erythrocytes. Lectin immobilization in MOF was effective for the construction of the electrochemical system, showing itself to be sensi-tive to conformational changes, being able to detect changes in the electrochemical poten-tials of Cramoll promoted in a buffered medium (200 mM potassium phosphate buffer, pH 7.0) with different glucose concentrations, highlighting the concentrations of 10 mM (96 - 121 mV), 15 mM (110 - 126 mV) and 20 mM (107 - 142 mV) in the time of 5 to 30 min. In the cyclic voltammetry, the interaction in the potential range from -0.2 to 0.0 V stood out; with the current reaching 0.0003 A and at peak 2 in the potential range of 0.4 to 0.5 V; with the current reaching 0.0007 A. The interaction of the carbohydrate present on the surface of the rabbit erythrocyte was also monitored by the potentiometric method, detecting changes in the Cramoll-glucose potentials, even in the presence of rabbit erythrocytes. Through SEM, it was possible to observe Cramoll immobilized on the MOF surface, proving the specificity of the ligand (glucose-lectin) through the morphological changes of this protein in this process. This electrochemical model, Cramoll/MOF biosensor, is effective for evalu-ating free lectin/carbohydrate or in the erythrocyte membrane.Item Síntese eletroquímica da estrutura metal-orgânica de [Cu3(BTC)2 .(H2O)3]n e sua aplicação em biossensores e meio ambiente(2018-08-27) Silva, Renata Pereira da; Freitas, Kátia Cristina Silva de; http://lattes.cnpq.br/9622769576689246; http://lattes.cnpq.br/9318114882701456The MOFs (Metal-Organic Frameworks) are a new class of materials known as porous coordination networks, form an extensive class of crystalline materials, featuring a central atom (metal ion) or sintered (metallic cluster) coordinated the organic molecules (ligands), in which this unit metal-ligand repeats itself forming a polymer metal complex network in one, two or three dimensions. One of the most important properties of this metal-organic framework is the high porosity and large surface area, properties that are exploited for the development of a textile dye adsorbent material in effluent and as crippling to the Lectin of Cratylia Mollis, a specific enzyme for glucose. The MOF [Cu3 (BTC) 2 ( H2O) 3] n was obtained by electrochemical synthesis and by current limiter using an acid solution 1, 3, 5-benzenotricarboxilico, sodium nitrate and dimethylformamide in water Mllipore a ratio of 1:1. The precipitate was filtered, washed and dried in an oven at 120° C, and then was characterized by absorption spectroscopy in the infrared region using a Fourier transform with spectrophotometer (FTIR) and by cyclic voltammetry. After this MOF mix with a solution that simulated a textile effluent, it was found that it can be used in your treatment as it was retrieved from a colorless effluent and the stabilization of the dye in your structure. The developed biosensors have been drawn up with the MOF with the lectin, and graphite paste in Platinum and gold electrodes. The cyclic voltammetry of these systems in solution of glucose and fructose. Indicating that the lectin interacts with low-concentration glucose solutions with the gold record (10:15, 5 mM due to the increase of the cathode and Anodic peaks), while that with the Platinum card she interacts with high concentrations of glucose (20, 40 and 60 mM due to the increase of cathode and Anodic peaks). Interaction not observed of the lectin with fructose, as expected. This system can be used for the identification of glucans in effluents, and identification of glucose in real samples, not requiring a large amount of sample for identification, making the low-cost and efficient method.