Licenciatura em Química (Sede)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/26
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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Resultados da Pesquisa
Item Síntese, caracterização e estudo citotóxico de um metaloprotótipo à base de rutênio 3+) com potencial atividade antileucêmica(2024-07-29) Moura, Severino Vitor do Nascimento; Silva, Wagner Eduardo da; Belian, Mônica Freire; http://lattes.cnpq.br/2626644337183959; http://lattes.cnpq.br/8612340815622413; http://lattes.cnpq.br/0613129512420118Ruthenium complexes constitute a promising class of metalloprototypes due to their biological activities. These ruthenium-based complexes have peculiar characteristics such as low associated toxicity, effective lability rate and “selectivity” to cancer cells, promoting damage to nuclear nDNA and apoptosis. Using strategies in combination with the Ru3+ ion and ligands with bioactive activities, the complex formed can show enhanced biological responses. This study aimed to synthesize, characterize and study the cytotoxicity of a metalloprototype containing ruthenium and ascorbate as a ligand. The synthetic methodology used enabled the formation of sodium ascorbate from the deprotonation of ascorbic acid with sodium bicarbonate in water, guaranteeing a reaction yield of 83%. The synthesized sodium ascorbate was then reacted with ruthenium (3+) chloride in water for 12 hours, resulting in the formation of the compound with the minimum formula [Ru(Asc)(OH2)4]Cl2 in a yield of 85%. This form was characterized by spectroscopy at infrared wavelengths, with significant differences in the absorption coefficients, such as the C=O and C=C bonds, when compared to ascorbic acid. The [Ru(Asc)(OH2)4]Cl2 complex was characterized by infrared spectroscopy, suggesting its formation through the shifts observed in the absorption bands of the C=O, C=C and C-O bonds. The characterization of the complex by electronic absorption spectroscopy showed hypsochromic shifts of the transitions located in the ligand of the π→π* type and the presence, in the visible, of transitions of the d→d type. The 1H NMR spectrum of the complex showed three signals, the multiplicities of which were two doublets and one triplet, the 13C NMR spectrum showed six signals consistent with the structure of the ligand used and the HSQC NMR spectrum showed three C-H correlation signals, also consistent with the complex studied. The high-resolution mass spectrum of the [Ru(Asc)(OH2)4]Cl2 complex showed six signals for the ruthenium isotopes (98Ru, 99Ru, 100Ru, 101Ru, 102Ru and 104Ru) with the highest relative m/z of 278.75, referring to the presence of Ru3+ and the ascorbate ligand [C6H9O6102Ru]+. The results of the cytotoxicity tests of the [Ru(Asc)(OH2)4]Cl2 complex, carried out on RAW 264.7 (normal cell line), MCF-7 (breast cancer) and HL-60 (human leukemia) cells, showed cytotoxic activity for the HL-60 cell line, with an IC50 of 39.3 μmol.L-1. For the normal cell line, the ruthenium complex proved to be non-cytotoxic (IC50 60.0 μmol.L-1). Therefore, the synthetic strategies used were satisfactory, with yields above 80%. The characterization techniques used were consistent with the formation of the complex obtained. The in vitro toxicological test showed evidence of non-toxicity to healthy cells and proved to be active in human leukemia cells (HL-60), possibly acting as an anti-leukemia candidate.Item Uma breve revisão dos complexos transplatínicos na terapêutica oncológica(2023-03-24) Oliveira, Barbara Daminnicy Nogueira de; Belian, Mônica Freire; Lima, Lidiane Macêdo Alves de; http://lattes.cnpq.br/9825403406472930; http://lattes.cnpq.br/2626644337183959; http://lattes.cnpq.br/4821392524886127Drugs with potential antitumor activity are of great interest to the pharmaceutical industry, since cancer is one of the diseases that affect thousands of people around the world. Cisplatin is a platinum (2+) compound widely used in cancer chemotherapy, with excellent rates of tumor regression, however, the severe side effects and cross-resistance limit its use. Thus, cisplatin analogues compounds such as carboplatin, nedaplatin, and oxaliplatin have been developed and clinically approved. Despite this, patients who use such drugs for treatment may develop cross-resistance. In this context, the need arose to search for new complexes that make it possible to minimize side effects and cross-resistance. Among the new platinum (2+) compounds of interest with antitumor activity studied, there are complexes with trans isomerism, which, due to their different mechanisms of action when binding to nDNA nucleophiles present promising strategies to overcome the mentioned difficulties encountered by current drugs with cis isomerism. Therefore, research on antitumor activity in platinum (2+) complexes with trans isomerism is relevant, to which this work is intended. This study consists of a brief review of the literature, allowing to map examples of transplatin complexes that stand out for their cytotoxic properties, configuring them as promising antineoplastic agents.Item Desenvolvimento de um protótipo antitumoral à base de boro-10 a ser aplicado na terapia de captura de nêutrons(2021-05-26) Sousa, Victor Branco de; Silva, Wagner Eduardo da; http://lattes.cnpq.br/8612340815622413; http://lattes.cnpq.br/4926809415572921In order to develop new prototypes capable of acting as neutron captors, this work presents the synthesis of a system based on polyvinyl alcohol and boric acid for future applications in neutron capture therapy and their characterizations carried out by thermogravimetric analysis, differential scanning of calorimetry, infrared spectroscopy and nuclear magnetic resonance of 1H, 13C and 11B. Two compounds were obtained, reproducible with the proposed methodology, with distinct physical and chemical characteristics, with a pH between 6.6 - 6.8. Thermal analysis data (TGA and DSC) corroborate to confirm the connections made between Boron and the polymer matrix. Boron-11 nuclear magnetic resonance data confirm the formation of three different species of boron, already described in the literature. The performance of the acute toxicity test via the OECD-423 protocol classifies the compound in category 4, showing good agreement for its future application in therapy.Item Cisplatina: uma revisão de literatura(2021-12-23) Pinto, Eddie William Andrews; Belian, Mônica Freire; http://lattes.cnpq.br/2626644337183959; http://lattes.cnpq.br/2493612296858465Cisplatin has been the subject of several studies since its discovery in the 1960s and used as a standard compound in evaluations of the effectiveness of platinum-based chemotherapy treatments. Analyzing the mechanisms of intracellular action of cisplatin, as well as the resistance mechanisms associated with it, is of fundamental importance, since many analogous com-pounds were and are elaborated until today in order to find less adverse effects and resistance to treatment, as well as greater specificity to tumor cells and more effective intracellular toxicity. For this, it is necessary to have knowledge about cisplatin, since its mechanisms of action on DNA is a pattern observed in this compound and its analogues from later generations. With these points in mind, this monograph aimed to identify some of the important productions on cisplatin in the last 5 years through a literature review. The methodology applied for the review consists of a series of steps ranging from the choice of topic to the final writing of the review, being extremely important for a good organization, selection, analysis, interpretation, writing. In all, 46 productions were chosen for a critical analysis. Topics were separated for the interpretation of the results of the analysis of the texts on cisplatin, namely: mechanism of action, adverse effects and new technologies. The 48 productions were organized and separated into topics according to the similar perspectives between the authors and a critical summary and interpretation of these works was performed. As final considerations, the review highlighted the importance of gathering such texts on cisplatin for study and instructions on the directions and trends of new technologies, such as the use of nanoemulsions and nanocarriers for cisplatin with the objective of increasing the efficiency of chemotherapy based on this compound and reduce the side effects associated with it.