Bacharelado em Ciências Biológicas (UAST)
URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2931
Siglas das Coleções:
APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso
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4 resultados
Resultados da Pesquisa
Item Associação entre fungos e abelhas-sem-ferrão do semiárido de Pernambuco(2021-06-25) Silva, Aparecida Clébia da; Carvalho, Airton Torres; http://lattes.cnpq.br/5995654016063333; http://lattes.cnpq.br/3574387196330564Throughout the evolution of species, insects and fungi have developed important mutualistic interactions, in which both benefit. An interesting example is the bee of the genus Scaptotrigona spp. (Hymenoptera: Apidae, Meliponini), in which a species of fungus Zygosaccharomycessp. develop and produce important metabolites for the growth of immatures, being essential for the insect to complete its life cycle. In this context, the objective was to investigate the presence of filamentous fungi, associated with larval food, in brood cells with larvae at different stages of development in colonies of Scaptotrigona sp. tubiba group, in Caatinga areas of the state of Pernambuco. The collections were carried out in five colonies in 10 brood cells, being numbered from 1 to 10, in which 1 to 5 were collected in cells with developing larvae, from 6 to 7 only with larval food and egg and from 8 to 10 developed larvae. The samples were solubilized in sterile water and seeded on three culture media for isolation Sabouraud Agar (SAB), Potato Dextrose Agar (PDA) and Potato Dextrose Agar with an additional 15% of glucose (15% PDA). After five days of incubation at 28 ºC, 19 isolates were selected based on morphotypes. Amongst these, 3 were identified at the genus level, being a Fusarium, a Cladosporium and an Arcopilus, which was submitted to macro and micromorphological characterization for presenting characteristics similar to a species described in the literature. Subsequently, the growth capacity of Arcopilus sp. in four different conditions: pure larval feed, larval feed plus distilled water (1:1; v/v), Sabouraud broth and distilled water plus 1% glucose in wells of ELIZA plates. The greatest growth was observed in wells that were seeded with larval feed diluted in distilled water. The presence of Monascus sp. in larval food samples of Scaptotrigona sp. studied. The other isolates were stored for later identification of the species and it is expected that these fungi may actually be essential in the bee nests of this species.Item Avaliação da produção de amilase e protease por fungos filamentosos de solos no Semiárido pernambucano(2019) Freires, Ariane Susan Santos; Costa, Cynthia Maria Carneiro; http://lattes.cnpq.br/9859547718446795; http://lattes.cnpq.br/0685613862543810The fungal soil communities suffer both quantitative and diversity interference caused by biotic and abiotic factors, and in the case of semi-arid soils, they are subject to low water availability, high temperatures and high solar radiation influences directly on the soil. development of these microorganisms. Thus, the objective of this work was to isolate and evaluate the enzymatic activity (amylase and protease) of filamentous fungi of Pernambucano semiarid soils collected at different times in the Mata da Pimenteira State Park Conservation Unit, in the municipality of Serra Talhada - PE. Soil collections were performed in August 2018 and April 2019, at three random points. Isolation of the fungi was performed by serial dilution technique using the Potato Dextrose Agar (BDA) and Sabouraud Agar (SAB) culture media added with chloramphenicol (for the inhibition of bacterial growth) in Petri dishes and incubated at room temperature. environment for seven days. After this period, the fungal colonies were quantified and, based on their morphotype, some were selected for the enzymatic tests of protease and amylase activity. As a result, 43 fungal colonies were isolated and a (CFU / g) quantification of (repeat 1), (repeat 2), (repeat 3) in BDA and SAB culture media, respectively, in the dry season and and (repeat 1), and (repeat 2), (repeat 3) in the BDA and SAB media respectively in the rainy season. Regarding the enzymatic potential 18 isolates were good producers of amylase and protease with emphasis on D8 identified as Aspergillus sp. which obtained greater degradation halos for both amylase and protease, with averages of 3.5 and 3.93 cm, respectively; and I3 also identified as Aspergillus sp. had a larger degradation halo for protease with 5.6 cm, both obtained during the drought period. In the rainy season, I9 (Isolated 4) and D9 (Aspergillus sp.) Stood out, which showed degradation halos of 3.5 and 4.16 cm for amylase and protease, respectively. The isolated fungi in this work have biotechnological importance, emphasizing how necessary is the preservation of habitats for the maintenance of microbial species associated with them.Item Coccidioidomicose no Brasil - uma revisão de literatura(2019) Santos, Cíntia Caline Bezerra dos; Costa, Cynthia Maria Carneiro; http://lattes.cnpq.br/9859547718446795; http://lattes.cnpq.br/2020390255494913Coccidioidomycosis (CMD) is a disease caused by Coccidioides immitis or Coccidioides posadasii and is associated with agricultural and hunting activities. These fungi preferentially inhabit regions of desert climates, produce infectious arthoconidia in the soil that can be aerosolized in the air and, when inhaled by humans, can cause systemic infections. Thus, the present study presents a literature review on coccidioidomycosis, addressing important aspects of the disease such as: characterization of the pathogen, epidemiology, diagnosis and treatment, as well as describing the history of this pathology in Brazil. Its elaboration was made possible through the use of national and international publications of articles, scientific journals, dissertations and theses in bibliographic databases such as: SCIELO (Scientific Electronic Library Online), VHL (Virtual Health Library), Portal CAPES and Pubmed, published in the last 30 years, from 1989 to 2019. Coccidioidomycosis is considered a public health issue, as it has a difficult diagnosis and treatment that is often nonspecific and is often confused with other respiratory diseases, wich can lead the patient to death. In 2018, Pernambuco had its first case of CMD reported in scientific literature in the municipality of Serra Talhada. Until 2007, cases of CMD were registered only in four northeastern states (Ceará, Piauí, Bahia and Maranhão).Item Fungos potencialmente micotoxigênicos em rações para animais domésticos comercializadas em Serra Talhada - PE(2019) Silva, Ana Gabrielle da Rocha e; Siqueira, Virgínia Medeiros de; http://lattes.cnpq.br/5524459744893735; http://lattes.cnpq.br/4575949447833727Due to the great domestic animal breeding in the world, the market of products directed to these animals, such asrations, has also increased. These feeds, in turn, may be subject to microbial contamination, mainly by fungi, which are microorganisms that can cause food deterioration or contamination by toxic substances such as mycotoxins, leading to financial losses and, when ingested, causing health risks to the animal. Thus, the objective of this work was to isolate, quantify and identify the filamentous fungi present in feed for dogs and cats commercializedin their original packageand bulk containers in the municipality of Serra Talhada / PE. Four samples sold in their originalpackage (two for dogs and two for cats) and four samples sold in bulk (twofor dogs and twofor cats) were analyzed using two different methods: serial dilution and direct plating for determination of quantity of Colony Forming Units (CFU) per gram of feed and the contamination index, respectively. All samples showed fungi of the generaAspergillusand Penicillium, witha maximum quantification of 36,7 x 104CFU / g of fungi for feed marketed in their original packaging, as well as for one sample of cat food thecontaminationindex was 100%. In general, rations sold in sealed packages had a higher amount of fungal counts than those sold in bulk, but there was no significant difference between the samples, according to other studies. In Brazil,there is no legislation that determines the maximum limit of fungal colonies per gram of feed, whatmakes it difficult to classify the sample as safe or not. However, the presence of Aspergillus and Penicillium under conditions of low water activity may indicate a possible production of mycotoxins in feed. Thus, it is determined that the form of commercialization is not the only factor that influences the fungal contamination of foods, although it is emphasized that the packaging protects the foods conserving their original characteristics and making them less prone to microbial contaminations.