Bacharelado em Ciências Biológicas (UAST)

URI permanente desta comunidadehttps://arandu.ufrpe.br/handle/123456789/2931

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APP - Artigo Publicado em Periódico
TAE - Trabalho Apresentado em Evento
TCC - Trabalho de Conclusão de Curso

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Atividade antimicrobiana de probióticos comerciais frente à enterobactérias prejudiciais ao homem
(2018) Santos, Larissa da Conceição; Costa, Cynthia Maria Carneiro; http://lattes.cnpq.br/9859547718446795; http://lattes.cnpq.br/1738865984772199
Probiotics are supplements of living microorganisms that confer benefits to the host. As a result of some change in the intestinal microbiota, probiotics are prescribed in an attempt to reverse imbalances caused by possible opportunistic pathogenic bacteria. Thus, in order to know the potential of probiotics marketed in pharmacy, Saccharomyces cerevisiae (Floratil®) Saccharomyces boulardii(Florax®) Bacillus cereus(Biovicerin®) and Bacillus clausii(Enterogermina®) was quantified in solid medium and compare with the manufacturer, to test the antimicrobial activity of probiotics individually, as well as the association of these with Lactobacillussp. (Isis ® fermented milk) in solid and liquid media against enterobacteria. The colony forming units count (UFC / mL) was performed by serial dilution and sowing in Petri dishes with Nutrient Agar (for bacteria) and Sabouraud Agar (for yeasts). The Petri dishes were incubated at 30 ° C for 24 and 48 hours for the growth of bacteria and yeasts and then quantification of the plaques that presented between 20 and 200 CFU / mL. The antimicrobial activity was evaluated from the growth of probiotics individually and their association with Lactobacillussp. in solid medium by the agar diffusion technique, which consisted in inoculating 15 mm discs onthe surface of the previously seeded Mueller Hinton agar, Escherichia coli, Salmonellasp. Klebsiellasp. and Citrobacter freundii. Antimicrobial activity of probiotic growth in liquid medium was also performed, with each probiotic extract in Mueller Hinton agar with the previously seeded bacteria. In addition, probiotic activity was also increased by the association of these with Lactobacillus sp. in liquid medium, in which, after their growth, each partner underwent two treatment conditions by placing 5 mL of the probiotic in two tubes of Falcon (15 mL) where one received ethanol (polar solvent) and the other 5 mL of acetate (apolar solvent) submitted to three hours under stirring, then 20 μl of the supernatant were pipetted from the tubes and inoculated onto the discs which were on the surface of the Mueller Hinton agar with the previously seeded test bacteria. All tests were performed in riplicates, incubated at 37ºC, with results obtained after 24 hours by the formation or not of the inhibition halo. There was no antimicrobial activity against the enterobacteria tested. Several factors should be taken into account when dealing with the metabolic action of microorganisms, under the conditions, in vitro, tested in this work.
Avaliação da produção de amilase e protease por fungos filamentosos de solos no Semiárido pernambucano
(2019) Freires, Ariane Susan Santos; Costa, Cynthia Maria Carneiro; http://lattes.cnpq.br/9859547718446795; http://lattes.cnpq.br/0685613862543810
The fungal soil communities suffer both quantitative and diversity interference caused by biotic and abiotic factors, and in the case of semi-arid soils, they are subject to low water availability, high temperatures and high solar radiation influences directly on the soil. development of these microorganisms. Thus, the objective of this work was to isolate and evaluate the enzymatic activity (amylase and protease) of filamentous fungi of Pernambucano semiarid soils collected at different times in the Mata da Pimenteira State Park Conservation Unit, in the municipality of Serra Talhada - PE. Soil collections were performed in August 2018 and April 2019, at three random points. Isolation of the fungi was performed by serial dilution technique using the Potato Dextrose Agar (BDA) and Sabouraud Agar (SAB) culture media added with chloramphenicol (for the inhibition of bacterial growth) in Petri dishes and incubated at room temperature. environment for seven days. After this period, the fungal colonies were quantified and, based on their morphotype, some were selected for the enzymatic tests of protease and amylase activity. As a result, 43 fungal colonies were isolated and a (CFU / g) quantification of (repeat 1), (repeat 2), (repeat 3) in BDA and SAB culture media, respectively, in the dry season and and (repeat 1), and (repeat 2), (repeat 3) in the BDA and SAB media respectively in the rainy season. Regarding the enzymatic potential 18 isolates were good producers of amylase and protease with emphasis on D8 identified as Aspergillus sp. which obtained greater degradation halos for both amylase and protease, with averages of 3.5 and 3.93 cm, respectively; and I3 also identified as Aspergillus sp. had a larger degradation halo for protease with 5.6 cm, both obtained during the drought period. In the rainy season, I9 (Isolated 4) and D9 (Aspergillus sp.) Stood out, which showed degradation halos of 3.5 and 4.16 cm for amylase and protease, respectively. The isolated fungi in this work have biotechnological importance, emphasizing how necessary is the preservation of habitats for the maintenance of microbial species associated with them.
Coccidioidomicose no Brasil - uma revisão de literatura
(2019) Santos, Cíntia Caline Bezerra dos; Costa, Cynthia Maria Carneiro; http://lattes.cnpq.br/9859547718446795; http://lattes.cnpq.br/2020390255494913
Coccidioidomycosis (CMD) is a disease caused by Coccidioides immitis or Coccidioides posadasii and is associated with agricultural and hunting activities. These fungi preferentially inhabit regions of desert climates, produce infectious arthoconidia in the soil that can be aerosolized in the air and, when inhaled by humans, can cause systemic infections. Thus, the present study presents a literature review on coccidioidomycosis, addressing important aspects of the disease such as: characterization of the pathogen, epidemiology, diagnosis and treatment, as well as describing the history of this pathology in Brazil. Its elaboration was made possible through the use of national and international publications of articles, scientific journals, dissertations and theses in bibliographic databases such as: SCIELO (Scientific Electronic Library Online), VHL (Virtual Health Library), Portal CAPES and Pubmed, published in the last 30 years, from 1989 to 2019. Coccidioidomycosis is considered a public health issue, as it has a difficult diagnosis and treatment that is often nonspecific and is often confused with other respiratory diseases, wich can lead the patient to death. In 2018, Pernambuco had its first case of CMD reported in scientific literature in the municipality of Serra Talhada. Until 2007, cases of CMD were registered only in four northeastern states (Ceará, Piauí, Bahia and Maranhão).
Fungos endofíticos em Bauhinia cheilantha (Bong.) Steud. Leguminosae, no Semiárido pernambucano
(2019) Leite, Isnaelia Gonçalves; Costa, Cynthia Maria Carneiro; http://lattes.cnpq.br/9859547718446795; http://lattes.cnpq.br/7234066240200376
Endophytic fungi are microorganisms that are present in plants and can colonize inter or intracellular various organs, establishing a mutualist relationship with the host. They can be associated with different plant species, including medicinal ones, producing secondary metabolites of biotechnological interest such as antibiotics. Therefore, the objective of this work was to isolate and taxonomically identify the endophytic fungi present in leaves of Bauhinia cheilantha (cowpaw, mororó) collected in Pernambuco semiarid, as well as to evaluate compound with antimicrobial potential. After random leaf collection and disinfection, 140 fragments were removed to isolate the endophytes in 20 Petri dishes containing Sabouraud Agar medium. Of these, 10 plates were kept at room temperature and 10 were stored in a greenhouse at 30 ° C for five days. Subsequently, isolation peaks were performed in pure culture for seven days. Thirty isolates of endophytic fungi were obtained, of which 17 were submitted to the antimicrobial activity test in Müeller Hinton agar solid medium against potentially pathogenic bacteria. No antimicrobial activity was observed under the tested conditions, however, five genera were identified: Alternaria sp., Bipolaris sp., Colletotrichum sp., Nigrospora sp. and Drecheslera sp., which constitute the first report, so far, endophytic fungi present in B. cheilantha for Pernambuco semiarid.
Quantificação, identificaçãoe bioprospecção de fungos cultiváveis, de solo em recuperação, no semiárido Pernambucano
(2018) Silva, Maiara Adriano da; Siqueira, Virgínia Medeiros de; Costa, Cynthia Maria Carneiro; http://lattes.cnpq.br/9859547718446795; http://lattes.cnpq.br/5524459744893735; http://lattes.cnpq.br/6266469936903681
Oil microbial communities suffer interference from biotic and abiotic factors, and in the case of semi-arid, these communities are subject to low water availability, high temperatures andhigh incidence of solar radiation. These conditions require high adaptation of these organisms, which may represent an important source of metabolites of interest to biotechnology. In this way, this work aimed at the study of the dynamics of the fungal community of soil in recovery of the semi -arid Pernambucano collected in the Conservation Unit State Park Mata da Pimenteira, in the municipality of Serra Talhada -PE. In order to do so, the soils were collected in two seasonal periods (one in the rainy season and two in the dry season) and the isolation and quantification of total fungi and thermophilic, osmotic and halophilic fungi were carried out.Antimicrobial and enzymatic activity tests of these fungi were also carried out. In collection 1 (rainy season) the highest quantification was obtained with 2.3 x 10⁷ CFU / mL of mesophilic fungi; from the same collection it was possible to isolate all groups. In collections 2 and 3 (drought period), the highest quantification was 7.33 x 10⁶ and 5.0 x 10⁶ CFU / mL of osmotic and mesophilic fungi, respectively. There was no isolation of halophilic fungi in samples 2 and 3. Of the 22 isolates selected and submitted to the test of antimicrobial activity, the isolate 11 (Fusarium sp.) With a halo of 8.33 mm in diameter was distinguished from Bacillus subtilis. Eighteen of the twenty fungi tested showed enzymatic activity with degradation of at least one tested substrate. These results allow establishinga correlation between the characteristics of the soils and the areas where they were collected, with the microbial populations found, besides valuing the biotechnological diversity and potential of the isolated microorganisms.