TCC - Engenharia Florestal (Sede)
URI permanente para esta coleçãohttps://arandu.ufrpe.br/handle/123456789/436
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Item CRISPR-Cas9 no setor florestal: uma abordagem cienciométrica(2021-12-09) Braz, Luísa Silva; Gallo, Ricardo; http://lattes.cnpq.br/5160912065817980; http://lattes.cnpq.br/2573015676942351CRISPR-Cas9 is a genetic editing technique that works like a pair of scissors, adding new information to the DNA after “cutting” it into a small, previously programmed part. This technological innovation brings great potential for the genetic improvement of plants, as it has a low cost and is simple to implement, when compared to other biotechnological techniques. Therefore, this work aimed to carry out a scientometric survey of research on CRISPR-Cas9 applied in the forest sector. Using the bibliometric method, the term “CRISPR-Cas9” combined with the following words was searched in the Web of Science and Scopus databases: "plant breeding", "tree", "forestry", "tree breeding"; “forest”. The articles obtained were analyzed on bibliometrix, generating data for scientometric analysis. A huge growth in the number of publications on the topic was observed, as of 2014. It was also found that 10 journals form the core of publications on the topic, according to Bradford's Law; and that the main publishers are: International Journal of Molecular Science, Frontiers in Plant Science and Plant Biotechnology Journal. The magazines with the greatest impact were: Plant Biotechnology Journal and Nature Communications. The most prominent authors were: Zhang Y, with 24 articles published, Li Y, with 19, Wang Y, with 17, Wang X, with 15 and Liu Y, with 11. In the analysis of Lotka's Law, we could see that 79 .3% of authors wrote only 1 article each on this topic, while few authors had a high number of publications. The survey also provided the researchers' affiliations. The University of California, South China Agricultural University, and University of Chinese Academy of Sciences are standind out with a high number of publications. The theme is being researched mainly in institutions in the United States and Asia, with research being carried out in Brazil. The words (Keywords Plus): "gene editing", "genetics", "plant breeding", "article" and "nonhuman" were the most cited in the articles, as for the keywords of the authors of the articles: tomato, potato, rice , Populus and Bombyx mori L., presented high frequency. Research involving CRISPR-Cas9 in forestry production is still in its beginning, noting that species of the genus Populus stood out in several studies, however it is possible to observe a considerable advance for agricultural crops, such as rice, tomatoes and potatoes. Forest species are still in the initial stages of using this technique, but they have great potential for improvement in clones and hybrids of species of commercial interest.Item Ciências “ômicas” aplicadas à análise de sementes(2020-11-05) Santana, Bárbara Jossany Gomes de; Santos, Marcone Moreira; Silva, Joselane Príscila Gomes da; http://lattes.cnpq.br/8028063388524508; http://lattes.cnpq.br/3284707164193715; http://lattes.cnpq.br/7128880355985474Seed analysis consists of the evaluation of seed quality through the use of different techniques for the most varied purposes. Many tools can be used, among them the “omics” sciences. This set of approaches can be considered a substantial technological advance for seed analysis. Within the techniques that are part of this set of science, the following stand out: transcriptomics (study of genetic transcripts, RNA‟s), proteomics (study of proteins), metabolomics (study of metabolites) and lipidomics (study of lipids). However, in relation to the forest seed analysis, these techniques are still not widespread. Thus, the objective of this work was to develop an exploratory study on the use of “omics” sciences for seed analysis, highlighting their objectives and technologies used. To this end, a database was created using Google academic as a search tool. Search terms were established in Portuguese and in English, which were: transcriptoma, proteoma, metaboloma, lipidoma para análise de sementes e para sementes florestais, transcriptomic, proteomic, metabolomic, lipidomic for seeds, for forest seeds analysis, for seeds analysis. The selected studies were grouped in tables according to the technique used: Transcriptomics, Proteomics, Metabolomics and Lipidomics. Species, purpose of the study, classification regarding use (Agricultural, Forestry, Medicinal and Others), origin of the studies (national or international) and year of publication were also identified. A total of 105 studies were analyzed, in which 64 corresponded to agricultural species, 18 forestry, 13 others and 10 were classified as medicinal. Between all the studies analyzed, 77% corresponded to international studies. Among the national studies, the most expressive techniques were: Transcriptomics and Proteomics with 38% and 28%, respectively. Regarding the year of publication, the oldest was Proteomics, in 2001. Regarding the classification of use for the species studied, the largest number of studies was developed with agricultural species, due to the greater development and investments of the sector, which has more resources for investment in advanced technologies. However, even if forest species are less studied, it is observed that the field of omics is a promising and developing area, and may have several applications in the future. Therefore, the importance of expanding investments in research in the field of forest seeds is emphasized, especially for native species, whose knowledge is incipient in view of the diversity and importance of species.