Navegando por Assunto "Bactérias"

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Ação antagonista de bactérias ácido láticas isoladas de queijos de coalho artesanal produzidos no agreste de Pernambuco
(2019-07-03) Silva, Juliana Bernardo da; Mendonça, Marcelo; http://lattes.cnpq.br/3517618342808435
Avaliação da cafeína no controle da infecção experimental de macrófago por Salmonella typhimurium
(2019) Almeida, Ingrydt de Alcântara; Lima Filho, José Vitor Moreira; Tavares, Lethicia Souza; http://lattes.cnpq.br/4128808335995892; http://lattes.cnpq.br/9476972124107533; http://lattes.cnpq.br/6015462685217823
Salmonellosis is an infectious disease transmitted mainly by foods contaminated with bacteria of the genus Salmonella, among them Salmonella enterica serotype Typhimurium, which can cause gastroenteritis until sepsis and septic shock in groups at risk. Organic compounds for pharmacological purposes are being increasingly tested. Among these compounds, caffeine (1,3,7-trimethylxanthine), one of the most used pharmacological and psychostimulant substances in the world. Severalstudies have demonstrated its role as a therapeutic adjunct, immunomodulator and adenosine receptor antagonist (ARs). In view of this, the objective was to evaluate the immunomodulatory potential of caffeine in peritoneal macrophages infected with Salmonella enterica Sor. Typhimurium. For this, in vitro tests were performed with cultures of macrophages exposed to caffeine concentrations to determine the degree of cytotoxicity of the compound; curative and preventive tests of cell viability, in order to evaluate cellular survival and quantification of intracellular bacteria, aiming to analyze the clearance of the pathogen from the intracellular medium. A direct antibacterial activity test was also carried out to evaluate the efficacy of caffeine in preventing the development of S. typhimurium. The results demonstrated that caffeine did not produce a toxic effect on the uninfected macrophages at the concentrations used, promoted a greater viability of the infected macrophages, but was not able to act as a direct bactericide. Caffeine contributed to the survival of macrophages infected with S. Typhimurium, having potential for the control of bacterial infections.
Avaliação das atividades antioxidantes de exopolissacarídeos (EPS) produzidos a partir de Enterococcus sp. isolado de queijo coalho artesanal
(2022-05-26) Santos, Sybelle Montenegro dos; Soares, Maria Taciana Cavalcanti Vieira; http://lattes.cnpq.br/3917225553030089; http://lattes.cnpq.br/2605906179220160
Exopolysaccharides (EPS) are macromolecules produced by different microorganisms, including lactic acid bacteria (LAB), as probiotic strains of Enterococcus. These have, in addition to bioactive properties, such as antioxidants, which neutralize oxidative stress in the host and reduce the use of artificial products harmful to health, proven therapeutic efficacy and high availability, are also indicated for several biotechnological applications. In this scenario, trends in healthy food consumption open new perspectives for the application of biopolymers such as EPS produced by BAL as additives and functional ingredients. Therefore, the objective was to produce, extract and evaluate the antioxidant activities of EPS produced by Enterococcus sp. isolated from artisanal coalho cheese from the State of Pernambuco, by scavenging the radicals: 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic (ABTS) and superoxide, through tests carried out in the laboratory, using EPS at the following concentrations: 0.2 0.5 1 1.5 and 2 mg/mL. The results obtained were applied in mathematical equations, according to each method used, and compared with ascorbic acid as a positive control. In addition, the mean and standard deviation were used for the ANOVA test with a significance of p < 0.05. Among the results found, it is remarkable the antioxidant activities developed by EPS 133v through its ability to eliminate free radicals. The DPPH radical reached a minimum of 16% (0.2 mg/mL) and in the maximum fraction (2 mg/mL) 27% of scavenging capacity, ABTS and superoxide presented a minimum of 56% and 42% and the maximum of 72% and 47%, respectively, however, the hydroxyl radical showed scavenging capacity only at the maximum concentration (2 mg/mL). Therefore, it is noticeable that the EPS 133v of Enterococcus sp. has high exploration potential for the development of functional food ingredients or additives with economic and health benefits.
Coleção de culturas bacterianas de rizosfera de Brachypodium spp
(2022) Mesquita, Aline Pinheiro; Oliveira, Frederico Inácio Costa de; http://lattes.cnpq.br/0588796892433431; http://lattes.cnpq.br/1171448892125076
Diversidade de genes de resistência em bactérias de ambientes extremos
(2022-10-07) Silva, Erivelton Gomes da; Freitas, Nara Suzy Aguiar de; http://lattes.cnpq.br/6891650997818766; http://lattes.cnpq.br/9369370749452563
Bacteria from extreme environments are poorly understood and the evolutionary histories linked to resistance and virulence gene patterns are still hidden. Although they are usually associated with a single extreme condition, they are often described as multi-resistances, which we assume is due to their rich genetic arsenal. Studying the diversity of these genes can help us to understand how bacterial life adapts in the scenario of environmental changes resulting from human action. This work studied the diversity of resistance mechanisms in bacteria and their shared genes between representatives of the Terrabacteria and Proteobacteria taxa. 16 genomes from 12 genera was selected, including thermophilic, psychrophilic, halotolerant, radiotolerant, acidophilic and resistant to heavy metals bacteria, in addition to 44 resistance genes. A phylogenetic tree was constructed with the 16S rRNA sequences (MEGA software). The sequences of the genes of interest were aligned against the NCBI/BLAST database, and their relationships to Mobile Genetic Elements (MGEs) obtained (IslandViewer 4). Among the gene products, we highlight the Quorum Sensing molecules for biofilm formation, present among phylogenetically distant taxa, where homologous signalers and receptors can be used to understand multi-resistances in extreme environments. On the other hand, we also found genes that act together in the creation of resistance, such as the mutS/mutL DNA repair genes, or the resistance genes to several phaE/phaC stressors, but which in some taxa showed the absence of one of alleles, or significant variations in the percentage of alignment of the alignments, indicating a possible difference in functionality. Other genes were more restricted to certain taxa, such as the ddrD of the radiotolerant Deinococcus radiodurans, which acts within a specific scenario of radiation and nutritional scarcity, in which case the improvement of a single gene/product led to a multi-resistance mechanism. Another example of restrictiveness is the phaE gene of the multidrug-resistant Rubrobacter xylanophilus, which cooperates in robustness and resistance to stress in this species. We also observed three cases of correlation between MGEs and resistance genes: the first in the occurrence of the radiotolerance gene recA in Genomic Islands in Thermus sp; another in the relationship of MGEs and Genomic Islands with the ars and cad genes, for arsenic and cadmium resistance, respectively, in Geobacillus stearothermophilus; and finally, the relationship of the Acidiphilium sp gene kdpB with plasmids in several of the taxa studied. This evidence indicates that, at least for a small part of these mechanisms, there is a potential for sharing resistance genes through Horizontal Gene Transfer (HGT). This potential for mobility could be an excellent biotechnological tool in the genomic editing of bacteria used in the bioremediation of contaminated environments. We believe that further studies of patterns and variations, phylogenetic analyzes and correlation of these genes with MGEs and genomic islands, may be ways to understand more about the diversity of resistance genes in extremophile bacteria.
Estudo dos parâmetros bioquímicos em Canistrum aurantiacum submetidas ao co-cultivo com bactérias promotoras de crescimento
(2025-02-26) Monteiro, Lucas Matheus da Silva; Silva, Cláudia Ulisses de Carvalho; Oliveira, Henarmmany Cristina Alves de; http://lattes.cnpq.br/6859724202937192; http://lattes.cnpq.br/7161911278790052; http://lattes.cnpq.br/6998551497198155
Este estudo investigou a influência de bactérias promotoras de crescimento (BPCs) do gênero Bacillus no metabolismo primário de Canistrum aurantiacum, uma espécie ornamental nativa de Pernambuco e vulnerável. A pesquisa focou em parâmetros bioquímicos como pigmentos fotossintéticos e carotenoides, carboidratos, aminoácidos, proteínas e nitrato, com o objetivo de avaliar os efeitos das bactérias na planta. Os resultados indicaram que a inoculação com BPCs não apresentou variações significativas nos teores de pigmentos, carboidratos ou proteínas, tanto nas folhas quanto nas raízes. No entanto, observou-se uma modulação dos aminoácidos nas raízes, com os grupos tratados apresentando uma estabilidade maior em relação ao grupo controle. O tratamento com Bacillus megaterium mostrou um aumento significativo nos teores de nitrato nas raízes, sugerindo uma melhoria na absorção de nutrientes. Este estudo fornece insights iniciais importantes para o uso de BPCs em programas de cultivo de Canistrum aurantiacum.
Estudo e caracterização de enterocinas obtidas por E. Faecium 137v e 141v para controle do desenvolvimento do câncer colorretal acompanhado por ultrassonografia
(2020-10-30) Aragão, Ana Beatriz Lins; Soares, Maria Taciana Cavalcanti Vieira; Calaça, Priscilla Régia de Andrade; http://lattes.cnpq.br/8351009381390103; http://lattes.cnpq.br/3917225553030089; http://lattes.cnpq.br/8961056711474591
Influência de fatores abióticos sobre o desenvolvimento de bactérias promotoras de crescimento vegetal dos gêneros Bacillus e Pantoea
(2019-02-05) Amorim, Alyson da Silva; Sobral, Júlia Kuklinsky; http://lattes.cnpq.br/8273377142633220; http://lattes.cnpq.br/9204041210789535
Incorrect handling during the application of most agricultural pesticides has been causing contamination in soils, groundwater, fauna and flora in Brazilian agroecosystems, in addition to increasing costs in agricultural production. The microbiota when subjected to dosages above the recommended level of certain pesticides suffers deleterious effects on their populations. And as a way to reduce input costs and the search for more sustainable agricultural production, the use of growth promoting bacteria and bioremediators is a biotechnological tool of great potential. Another factor that influences agricultural production is salinity. It is a term that qualifies a situation of excess soluble salts in the environment. This problem is one of the main causes of soil degradation in regions of arid and semi-arid climate, culminating in serious agricultural income. To contribute to the recovery of these degraded soils, plant growth promoting bacteria have been shown to be useful in the development of strategies to promote plant growth in saline soils. In view of the above, the present work had as objectives: i) to evaluate the tolerance to the methomyl pesticide and the potential biodegradation capacity of this pesticide by Bacillus plant growth promoting bacteria; and ii) to evaluate the production of exopolysaccharides under saline stress by plant growth promoting bacteria of the genus Pantoea. For the evaluation of pesticide tolerance, the Bacillus bacteria (UAGAT35 and UAGAT71). were inoculated in modified Minimal Mineral Medium (MMM), plus glucose as carbon source, followed by different concentrations of Methomyl. And to assess the potential for biodegradation, the carbon source has not been increased. In the experiment on salinity, five bacterial strains of the genus Pantoea were used (UAGC 906, UAGC 977, UAGC 858, UAGC 907 and UAGC 972), which were grown in solid medium with sucrose and added different concentrations of NaCl in each tr. For 10 days, the growth of the halo was measured with the aid of a pachymeter, being done five readings, in intervals of 48 hours. The two strains of the genus Bacillus demonstrated tolerance and biodegradation to methomyl insecticide, in their different concentrations. While the 5 bacterial strains of the genus Pantoea submitted to the different concentrations of NaCl presented production of Exopolysaccharides (EPS), showing their survival in environments under IX salt stress. Therefore, the evaluated bacteria presented potential for future studies aiming to promote growth in adverse environments.
Intoxicação alimentar por histamina associada ao consumo de peixe no Brasil: revisão de literatura
(2019-12-13) Matias, Gilmara do Nascimento; Franque, Marcos Pinheiro
Microbiota bacteriana associada a pele de Scinax x-signatus (Spix, 1824) (Anura: Hylidae) em Pernambuco, Brasil
(2020-02-03) Santos, Lara Valesca Mendonça da Costa; Santos, Alcina Gabriela Maria Medeiros da Fonsêca; Moura, Geraldo Jorge Barbosa de; http://lattes.cnpq.br/1348666346504103; http://lattes.cnpq.br/4938571253882757; http://lattes.cnpq.br/6095901652697979
The presence of a symbiotic microflora in extremely sensitive animals like amphibians is crucial for the development of an immune system capable of fighting the main opportunistic infectious agents in the environment. Scinax x- signatus is a anuro which has a general and synanthropic behavior occurring in forests and urban areas, but there are no identification studies of bacterial microbiota associated with skin of this animal. The objective of this study was to identify the skin microbiota of Scinax x-signatus in two areas in the Botanical Garden Reef (08 º 04’ S, 34 º 59’ W) and compare the resident and transient microorganisms. Rinses were performed with sterile distilled water to remove the animals from transient microflora and swabs wiped on the skin to collect the resident microflora. Samples were predictively you identify in selective media, as Chromogenic Agar, Cetremide, EMB Agar and MacConkey agar For the analyzes, we used Fisher Test and Cluster Analysis to verify the similarity between the collection areas. 24 sample were isolated from bacterial microflora and predictive methods were taxa 5: Pseudomonas spp. (43%) occurring in most samples, Escherichia coli (22%), Klebsiella pneumoniae (18%), Enterococcus faecalis (12%) and Staphylococcus aureus (4%), with the lower frequency. The microbiota from the anthropized area showed less similarity between the resident and transient samples. Cluster Analysis indicated that the microbiota from the control area was more similar among transients and residents samples, possibly due to the low degree of spatial heterogeneity. Scinax x-signatus specimes presented skin microorganisms of medical interest that are similar to other species of frogs. S. x-signatus has great ecological importance to ecosystems, and studies to research antimicrobial potential on the skin and in the microbiota associated with S. x-signatus are very important for the production of pharmaceuticals.
Produção, extração, purificação e caracterização de proteases fibrinolíticas produzidas por Streptomyces parvulus DPUA 1573
(2021-11-29) Nascimento, Maria Clara do; Bezerra, Raquel Pedrosa; Batista, Juanize Matias da Silva; http://lattes.cnpq.br/6699725036732885; http://lattes.cnpq.br/1466206759539320; http://lattes.cnpq.br/5929405825655717
Due to their fibrin degradation potential, fibrinolytic proteases are a promising alternative in the pharmaceutical industry for the treatment of cardiovascular diseases, especially thrombosis. There are several sources of fibrinolytic proteases, however, the microbial sources are the ones that stand out in terms of low cost and high production rates. From their production to application, enzymes need to go through several processes, which sounds negative, making the steps more costly and late. A method capable of overcoming these problems is the aqueous two-phase system (SDFA), a process capable of reducing downstream steps. The objective of this work was to produce, purify and biochemically characterize the fibrinolytic protease produced by Streptomyces parvulus DPUA 1573. The protease was produced by submerged fermentation using agro-industrial waste or co-products. The crude extract that showed the highest enzymatic activity (passion fruit peel flour) was subjected to extraction by SDFA consisting of polyethylene glycol (PEG) and phosphate salts (potassium and sodium), following a 24 plan. After extraction by SDFA, the protease was subjected to purification by gel filtration chromatography, and already purified had its biochemical characterization performed. The protease produced by S. parvulus DPUA 1573 showed fibrinolytic activity of 15.46 U/mL and was able to form a halo of 317.31 mm2 acting on fibrin degradation. In SDFA, the fibrinolytic protease partitioned preferentially to the PEG-rich phase. The best assay selected according to the combination of the highest specific activity index, purification factor and activity yield was 16, composed of PEG 8,000 gmol-1, 17.5 v/v PEG, pH 8.0 and 15 v/v of phosphate salts. The protease activity of the enzyme was highly stimulated in the presence of iron, reaching a 55% increase in activity, and drastically decreased in the presence of the protease inhibitor 2-mercaptoethanol (91%). The optimum temperature and pH for the enzymatic activity were 40ºC and pH 7.0, respectively, keeping the enzyme activity stable between 30ºC and 60ºC and in the pH range from 7.0 to 8.5. Based on the analyzed results, it was seen that S. parvulus DPUA 1573 proved to be a good producer of fibrinolytic proteases, and the PEG/Phosphate aqueous two-phase system proved to be a great alternative for the extraction and pre-purification of fibrinolytic proteases.
Relatório final de atividades do Estágio Supervisionado Obrigatório: seleção de bactérias com potencial de utilização em processo de biorremediação de solos contaminados por mercúrio
(2025) Oliveira, Jennifer Nicoli de Souza; Biondi, Caroline Miranda; http://lattes.cnpq.br/8326756664758702; http://lattes.cnpq.br/1940730909264239
Os manguezais desempenham um papel crucial na ecologia, manutenção da qualidade da água, biodiversidade e estabilidade dos solos. No entanto, a contaminação por metais pesados, como o mercúrio (Hg), tem se mostrado um problema decorrente das atividades industriais. Atualmente tem se buscado formas de recuperar essas áreas contaminadas por Hg, sendo a biorremediação microbiana uma alternativa promissora para recuperação dessas áreas, pois utiliza de bactérias indígenas, resistentes ao Hg capazes de volatilizá-lo. Por isso, objetivou-se neste trabalho avaliar a eficiência de bactérias indígenas de solo contaminado por Hg no estuário do Rio Botafogo, para uso em processos de biorremediação. Foram coletadas amostras de solo do estuário do Rio Botafogo, em Pernambuco, divididas em P1 e P2, e isoladas bactérias dessas amostras. Foram realizados testes como o de Concentração inibitória mínima (CIM) para avaliar a capacidade das bactérias em resistir ao mercúrio. Os resultados indicaram que os solos do estuário estão contaminados por Hg em concentrações de 4,28 a 10,42 mg kg-1 e que há a presença de isolados dos gêneros Enterococcus, Bacillus e Pseudomonas, que possuem resistência de 50 a 100 mg L-1 de Hg, de acordo com o teste CIM. Essas bactérias apresentam potencial para serem utilizadas em processos de biorremediação. Além disso, o solo do manguezal do estuário do Rio Botafogo apresentou pH próximo à neutralidade e potencial redox indicando ambiente anóxico.