Navegando por Autor "Souza, Paulo Roberto Eleutério de"

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Análise da prevalência do câncer de próstata em pacientes atendidos no Laboratório Municipal de Saúde Pública do Recife (2015 - 2022)
(2024-10-03) Costa, José Rodolfo Paixão da; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/3567527582772343
A Organização Mundial de Saúde (OMS) considera o câncer de próstata como um problema de saúde pública, e segundo dados do INCA somente em 2022, foram registrados 15.841 mil óbitos no Brasil. E segundo estimativas do INCA, o Nordeste ocupa o segundo lugar em ranking de casos, com 73,28 casos a cada 100 mil habitantes (INCA 2023). No estado de Pernambuco apenas no ano de 2023 estimou-se 2.930 novos casos por 100 mil habitantes, destes, 550 novos casos para a cidade do Recife (INCA, 2023). Contudo, as informações sobre prevalência a nível regional desta doença são escassas e precisam ser mais bem estudadas. O presente estudo teve como objetivo estimar a prevalência de câncer de próstata em homens atendidos no ambulatório do Laboratório Municipal de Saúde Pública da Secretaria de Saúde do Recife no período de 2015 a 2022. O estudo teve embasamento retrospectivo com apuração e levantamento de dados de prontuários de biopsiasrealizadas no Laboratório Municipal de Saúde Pública no período de 2015 a 2022. Foram analisados 15.506 prontuários, destes 1.808 foram relacionados com biópsias de próstata, correspondendo a 11,66% dos prontuários totais. Além disso, 821 prontuários foram diagnosticados como positivos, correspondendo a 45,4% dos prontuários. Em relação à variável epidemiológica idade, observou-se que a maior parte deles estava na faixa etária entre 60 e 79 anos. Além disso, verificamos uma maior recorrência na região terço média direita do adenocarcinoma na zona periférica do tecido prostático. Desta forma, o presente estudo verificou aprevalência de câncer de próstata no Laboratório Municipal de Saúde Pública, do Recife nos últimos sete anos, e os dados encontrados podem ajudar na adoção de medidas de intervenção mais eficazes para população masculina regional, assim como, ajudar no rastreio de câncer de próstata em determinadas zonas prostáticas auxiliando assim no tipo de tratamento para o paciente acometido.
Análise de colinearidade gênica do operon aprX-lipA em isolados de Pseudomonas fluorescens
(2023-09-15) Silva, Israel Santos da; Freitas, Nara Suzy Aguiar de; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/6891650997818766; http://lattes.cnpq.br/9803828236017805
Pseudomonas fluorescens are Gram-negative bacilli that are motile and found in terrestrial and aquatic ecosystems. Due to their psychrotrophic characteristics, these bacteria are often associated with the contamination of unpasteurized milk and its derivatives, such as cheese and butter. The proteases and lipases produced by P. fluorescens are the primary factors in the prevalence of dairy product contamination. These enzymes are encoded by the aprX and lipA genes, which are present in the aprX-lipA operon. In this regard, we evaluated the genomic components surrounding the aprX-lipA operon of P. fluorescens from different sources, aiming to detect genetic patterns inherent to these organisms and their correlation with proteolytic activity. We used the NCBI database, the String platform, and Interpro for comparative evaluation of the selected genomes. In the four isolates analyzed, the aprX-lipA operon is highly variable structurally, with unique configurations for each genome. The gene co-expression relationships of the genes surrounding the aprX protease also show qualitative and quantitative variations, both intra- and inter-species within the Pseudomonas genus. We detected components of the CRISPR type 1 system, previously unrelated to the operon, which can amplify, move, and modify genes related to the defense mechanism, pathogenic or not, of the genus. The patterns related to pathogenicity indicate that new biomarkers can be used for genomic surveillance.
Análise de incidência de câncer de vesícula biliar em pacientes atendidos no Laboratório Municipal de Saúde Pública do Recife (2014 - 2022)
(2024-02-05) Santos, Bruna Maria Correia dos; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/2311174575605049
Gallbladder cancer (GBC) is considered a rare type of cancer by the World Health Organization (WHO) and characterized by the absence of symptoms in the early stages, but in advanced stages manifests with abdominal pain. The WHO considers this type of tumor as the most common in the biliary tract, associating it with risk factors such as gallstones, age, female sex, and a higher propensity in white individuals. This study aimed to evaluate the prevalence of gallbladder cancer in patients undergoing elective cholecystectomies, assessing the occurrence of GBC in patients treated at the Municipal Public Health Laboratory of the Recife- Pernambuco from 2014 to 2022. An observational, retrospective, descriptive-analytical study was conducted using secondary sources of patients diagnosed with GBC from 2014 to 2022. A total of 17,970 medical records were analyzed during the study period. Among these, 10 were related to the gallbladder, and only 2 had a diagnosis of GBC. The female sex was present in 70% (7/10) of the cases and 50% were diagnosed with gallstone disease. The most performed operation was simple cholecystectomy. In the two patients with GBC, the diagnosis of gallbladder adenocarcinoma was confirmed. Tumor staging revealed 50% (1/2) at stage IIIb and 50% (1/2) at stage II among the examined cases. Thus, this study confirmed the prevalence of gallbladder adenocarcinoma in GBC and identified cholelithiasis as the main risk factor similar to previous studies.
Desenvolvimento do aplicativo Endocanabinóide: a importância da divulgação científica da Cannabis medicinal em mídias digitais
(2021-12-10) Silva, Adrielly Ferreira das Neves; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/2123961681874042
Science and technology are essential for the socioeconomic development of a society, and for understanding the complexity of the world through scientific education to citizens so that they recognize science as an integral part of their culture. In this context, the internet and its digital platforms work as enhancers for the accessibility of scientific and technological information. Currently, a lot of information about the medicinal use of Cannabis sativa L. is being published in the most diverse media, but this information does not always have a scientific basis. Most of society is uninformed of the implications of this plant in the treatment of various pathologies, especially in the treatment of neurodegenerative diseases. The objective of this work was to create a mobile app, the Endocannabinoid, with scientific information, adopting communication strategies in simple and accessible language. The FabApp website was used to create the application, academic research platforms, such as Google, Scielo and NCBI, to reference and Canva to create teaching materials, such as images, visual content and logo. Thus, the development of this application made it possible to scientifically inform people about the medicinal use of Cannabis sativa L., and the Laws governing the cultivation and medicinal use of the plant, and it has contributed to improving the quality of life of people suffering from diseases which can be treated with the active principle of this plant.
Estudo de polimorfismos no gene receptor da vitamina D em pacientes com a doença de Alzheimer
(2021-12-09) Nascimento, Higor Vinicius Alves do; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/2776608155510414
Vitamin D (VD) is a neurosteroid whose function is related to cell regulation, ion homeostasis, growth regulation and protective effect against injuries. Its deficiency may be associated with neurological disorders such as Alzheimer's disease. The effect of VD is exercised by the interaction with VD receptor, the VDR. The presence of the VDR gene is considered highly expressive, especially in the encephalon, hypothalamus and nigra substance, indicating a significant participation in the functioning of the nervous system. The VDR gene is located on the chromosome 12q13.11 and presents 14 exons. Polymorphisms in this gene have been associated with changes in its function and may influence its effects. The polymorphisms FokI (rs2228570) and TaqI (rs731236) are located in exotic regions and the BsmI (rs1544410) is located in the intronic region. Thus, this study aimed to verify the prevalence of BsmI, TaqI and FokI polymorphisms in the VDR gene in individuals with Alzheimer's disease (AD) and healthy control individuals (CS), in a population of the state of Pernambuco. A total of 78 individuals were evaluated, 39 with Alzheimer’s disease and 39 healthy controls treated in the outpatient clinic of the Hospital das Clínicas of UFPE and the characterization of genotypes was performed using the PCR-RFLP technique. The frequencies of FokI genotypes were 48.9% CC, 46.1% CT, 5% TT for the AD group and 43.6% CC, 53.9% CT and 2.5% TT for the CS group. Regarding genotypes BsmI, 15.4% AA, 53.9 AG and 30.8% GG were observed for the group with AD and 10.3% GG, 56.4% AG and 33.3% AA for the CS group. In turn, the frequencies of the TaqI genotypes for the AD group were 51.3% CT and 48.7% CC while for the CS group they were 61.5% CT and 38.5% CC. No significant difference was found when statistical analyses were performed between the groups (AD x CS) [p >0.05 for each analysis performed]. Thus, we can conclude that none of these three polymorphisms in the VDR gene seems to be involved with the susceptibility to AD in the studied population. However, new studies, including a larger number of patients, may elucidate our observed results.
Genética geral para universitários
(EDUFRPE, 2015) Souza, Paulo Roberto Eleutério de; Silva, Hildson Dornelas Angelo da; Leite, Fernanda Cristina Bezerra; Maia, Maria de Mascena Diniz; Garcia, Ana Cristina Lauer; Montes, Martín Alejandro
Desde ano de 2008, vimos programando editar um livro de genética geral, para ser usado pelos alunos dos cursos de Agronomia, Engenharia Florestal, Veterinária, Zootecnia, Biologia (Licenciatura e Bacharelado) da UFRPE. A intenção foi descrever os temas do conteúdo da disciplina Genética Geral, em uma linguagem simples, lógica e sintética com o objetivo de facilitar o aluno na compreensão dos conceitos básicos de cada assunto abordado pertinente ao processo de aprendizagem da genética. Essa ideia nasceu da experiência como professores de genética sempre ouvindo dos alunos que essa disciplina é “muito difícil”, os “livros trazem uma linguagem complexa” os “assuntos envolvem matemática” os “capítulos são longos” entre outras dificuldades. Assim, em 2011, resolvemos elaborar um livro para ser publicado pela Editora da Universidade Federal Rural de Pernambuco. Desejamos, portanto, que os nossos alunos em contato com os assuntos que escrevemos sejam estimulados a aprender genética de uma forma pessoal relacionando os conteúdos a algo que eles já conhecem e se deparam no dia-a-dia associando os fatos do passado com o presente de modo a tornar os temas mais interessantes no que se refere à beleza sobre o panorama da vida.
PPARs no câncer de fígado: sintenia e interações aplicados a medicina evolutiva
(2024-02-26) Leôncio, Thays Maria; Freitas, Nara Suzy Aguiar de; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/6891650997818766; http://lattes.cnpq.br/3551659636270655
Liver cancer is the sixth most common diagnosis and the third cause of death. Peroxisome proliferator-activated nuclear receptors (PPARs) are a group of genes that act as transcription factors and have recently been indicated as potential therapeutics for liver cancer. Thus, we studied the PPARs genes associated with their interactions and synteny in an evolutionary medicine approach to liver cancer. The selection of the PPAR family and the primary analysis of their functions were obtained on the NCBI and UniProt platforms. The search for gene interactions was carried out using the protein association network in the STRING database, for the PPAR, PPAR and PPAR subtypes and the synteny analysis of this gene group was carried out. The return of information indicated that the set of genes and gene products evaluated have multiple functionalities of varying magnitude and complexity, being related to liver metabolism and its risk factors or not with liver cancer. In addition to being Mendelian units, they also mainly present positive and negative pleiotropic effects, directly or indirectly involved with liver diseases or healthy phenotypes, such as activities related to thyroid function, spermatogenesis, bone formation and differentiation, satiety control, circadian cycle, among others. These analyzes that are just beginning show promise for an early prognosis involving not only the molecular interactions of PPARs, but their positions in the genome. In this sense, genes are also modulated sometimes to develop their tumorigenic expression, sometimes not, suggesting that their study focused mainly on a healthy phenotype can facilitate preventive actions. This basic approach to evolutionary medicine identified new biomarker targets for hepatocellular carcinoma that could be effectively useful for future genetic evaluations, aiming to better prevent diseases and promote health.
Relação da expressão da chemerina placentária com biomarcadores de perfil metabólico em vacas no momento do parto
(2024-07-11) Dantas, João Victor José de Barros; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/7857759665969205
The calving period is a critical time for cows, characterized by high energy demands and negative energy balance, which increases susceptibility to metabolic disorders such as hyperketonemia and hypocalcemia, negatively affecting animal health and immunity. This study investigated the relationship between chemerin protein expression and various metabolic biomarkers at calving, considering the hypothesis that chemerin could serve as a substitute or auxiliary marker for existing biomarkers due to its role in metabolic and inflammatory processes. Blood and placental tissue samples were collected from 14 pregnant cows, subjected to clinical examinations and collection of blood and placental tissue. Biochemical and hormonal parameters were analyzed from blood samples, while chemerin gene expression was evaluated from placental tissue samples. The results showed a moderate negative correlation between chemerin expression and serum sodium concentration (r=-0.60; p=0.0241), suggesting a possible regulatory role of chemerin in electrolyte balance during calving. Additionally, significant positive correlations were found between the energy biomarkers BHB and NEFA, and between the protein profile biomarkers total protein and globulin, reflecting the metabolic state during the peripartum period. However, no significant direct correlations were observed between chemerin expression and most of the other metabolic biomarkers studied. It is concluded that chemerin expression in placental tissue may have a regulatory role in electrolyte balance, but its influence on other aspects of the metabolic profile is limited and requires further investigation. Future research should focus on elucidating the mechanisms by which chemerin may influence metabolism and homeostasis during calving.
O transplante de células tronco hematopoiéticas como tratamento da anemia falciforme: uma revisão de literatura
(2022-09-30) Oliveira, Luís Eduardo Rangel Batinga de; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/9122060791637391
Uso dos sistemas CRISPR no diagnóstico do câncer
(2024-10-03) Coura Filho, Fábio de Souza Esteves; Souza, Paulo Roberto Eleutério de; http://lattes.cnpq.br/1971832245117283; http://lattes.cnpq.br/9573756167234737