Navegando por Autor "Santos, Lara Valesca Mendonça da Costa"

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    Microbiota bacteriana associada a pele de Scinax x-signatus (Spix, 1824) (Anura: Hylidae) em Pernambuco, Brasil
    (2020-02-03) Santos, Lara Valesca Mendonça da Costa; Santos, Alcina Gabriela Maria Medeiros da Fonsêca; Moura, Geraldo Jorge Barbosa de; http://lattes.cnpq.br/1348666346504103; http://lattes.cnpq.br/4938571253882757; http://lattes.cnpq.br/6095901652697979
    The presence of a symbiotic microflora in extremely sensitive animals like amphibians is crucial for the development of an immune system capable of fighting the main opportunistic infectious agents in the environment. Scinax x- signatus is a anuro which has a general and synanthropic behavior occurring in forests and urban areas, but there are no identification studies of bacterial microbiota associated with skin of this animal. The objective of this study was to identify the skin microbiota of Scinax x-signatus in two areas in the Botanical Garden Reef (08 º 04’ S, 34 º 59’ W) and compare the resident and transient microorganisms. Rinses were performed with sterile distilled water to remove the animals from transient microflora and swabs wiped on the skin to collect the resident microflora. Samples were predictively you identify in selective media, as Chromogenic Agar, Cetremide, EMB Agar and MacConkey agar For the analyzes, we used Fisher Test and Cluster Analysis to verify the similarity between the collection areas. 24 sample were isolated from bacterial microflora and predictive methods were taxa 5: Pseudomonas spp. (43%) occurring in most samples, Escherichia coli (22%), Klebsiella pneumoniae (18%), Enterococcus faecalis (12%) and Staphylococcus aureus (4%), with the lower frequency. The microbiota from the anthropized area showed less similarity between the resident and transient samples. Cluster Analysis indicated that the microbiota from the control area was more similar among transients and residents samples, possibly due to the low degree of spatial heterogeneity. Scinax x-signatus specimes presented skin microorganisms of medical interest that are similar to other species of frogs. S. x-signatus has great ecological importance to ecosystems, and studies to research antimicrobial potential on the skin and in the microbiota associated with S. x-signatus are very important for the production of pharmaceuticals.